Heat Shock 70kDa Binding Protein 1 (HSPBP1)
[Edit]FES1; Hsp70 Interacting Protein; Hsp70 Binding Protein,Cytoplasmic Cochaperone 1; Heat shock protein-binding protein 1; Hsp70-interacting protein 1
Using the ATPase domain of human HSP70 as bait in a yeast 2-hybrid screen of a human heart cDNA library, Raynes and Guerriero (1998) cloned HSPBP1. The deduced 459-amino acid protein has a calculated molecular mass of 39.3 kD and contains 6 consecutive glycines near its N terminus. Northern blot analysis detected a 1.7-kb HSPBP1 transcript in all tissues examined, with highest expression in heart and skeletal muscle.
Raynes and Guerriero (1998) also identified an HSPBP1 variant that they called HSPBP2, which has 2 polyadenylation signals rather than the single polyadenylation signal of HSPBP1. The deduced HSPBP2 protein is identical to HSPBP1 except that it has 9 consecutive glycines near the N terminus.
Organism species: Homo sapiens (Human)
CATALOG NO. | PRODUCT NAME | APPLICATIONS | |
Proteins | RPD506Hu01 | Recombinant Heat Shock 70kDa Binding Protein 1 (HSPBP1) | Positive Control; Immunogen; SDS-PAGE; WB. |
Antibodies | PAD506Hu01 | Polyclonal Antibody to Heat Shock 70kDa Binding Protein 1 (HSPBP1) | WB; IHC; ICC; IP. |
LAD506Hu71 | Biotin-Linked Polyclonal Antibody to Heat Shock 70kDa Binding Protein 1 (HSPBP1) | WB; IHC; ICC. | |
MAD506Hu21 | Monoclonal Antibody to Heat Shock 70kDa Binding Protein 1 (HSPBP1) | WB; IHC; ICC; IP. | |
Assay Kits | SED506Hu | ELISA Kit for Heat Shock 70kDa Binding Protein 1 (HSPBP1) | Enzyme-linked immunosorbent assay for Antigen Detection. |
Organism species: Mus musculus (Mouse)
CATALOG NO. | PRODUCT NAME | APPLICATIONS | |
Proteins | RPD506Mu01 | Recombinant Heat Shock 70kDa Binding Protein 1 (HSPBP1) | Positive Control; Immunogen; SDS-PAGE; WB. |
Antibodies | PAD506Mu01 | Polyclonal Antibody to Heat Shock 70kDa Binding Protein 1 (HSPBP1) | WB,IHC |
LAD506Mu71 | Biotin-Linked Polyclonal Antibody to Heat Shock 70kDa Binding Protein 1 (HSPBP1) | WB; IHC; ICC. | |
Assay Kits | n/a | CLIA Kit for Heat Shock 70kDa Binding Protein 1 (HSPBP1) | CLIA Kit Customized Service Offer |
n/a | ELISA Kit for Heat Shock 70kDa Binding Protein 1 (HSPBP1) | ELISA Kit Customized Service Offer |
Organism species: Rattus norvegicus (Rat)
CATALOG NO. | PRODUCT NAME | APPLICATIONS | |
Proteins | RPD506Ra01 | Recombinant Heat Shock 70kDa Binding Protein 1 (HSPBP1) | Positive Control; Immunogen; SDS-PAGE; WB. |
Antibodies | PAD506Ra01 | Polyclonal Antibody to Heat Shock 70kDa Binding Protein 1 (HSPBP1) | WB; IHC; ICC; IP. |
LAD506Ra71 | Biotin-Linked Polyclonal Antibody to Heat Shock 70kDa Binding Protein 1 (HSPBP1) | WB; IHC; ICC. | |
CAD506Mi01 | Anti-Heat Shock 70kDa Binding Protein 1 (HSPBP1) Polyclonal Antibody | Loading Control of WB. | |
MAD506Ra23 | Monoclonal Antibody to Heat Shock 70kDa Binding Protein 1 (HSPBP1) | WB; IHC; ICC; IP. | |
MAD506Ra21 | Monoclonal Antibody to Heat Shock 70kDa Binding Protein 1 (HSPBP1) | WB; IHC; ICC; IP. | |
MAD506Ra22 | Monoclonal Antibody to Heat Shock 70kDa Binding Protein 1 (HSPBP1) | WB; IHC; ICC; IP. | |
Assay Kits | n/a | CLIA Kit for Heat Shock 70kDa Binding Protein 1 (HSPBP1) | CLIA Kit Customized Service Offer |
n/a | ELISA Kit for Heat Shock 70kDa Binding Protein 1 (HSPBP1) | ELISA Kit Customized Service Offer |
- "Inhibition of Hsp70 ATPase activity and protein renaturation by a novel Hsp70-binding protein."J. Biol. Chem. 273:32883-32888(1998) [PubMed] [Europe PMC] [Abstract]
- "Isolation and characterization of isoforms of HspBP1, inhibitors of Hsp70."Biochim. Biophys. Acta 1490:203-207(2000) [PubMed] [Europe PMC] [Abstract]
- "Large-scale cDNA transfection screening for genes related to cancer development and progression." Proc. Natl. Acad. Sci. U.S.A. 101:15724-15729(2004) [PubMed] [Europe PMC] [Abstract]
- "Complete sequencing and characterization of 21,243 full-length human cDNAs." Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
- "Signal sequence and keyword trap in silico for selection of full-length human cDNAs encoding secretion or membrane proteins from oligo-capped cDNA libraries." DNA Res. 12:117-126(2005) [PubMed] [Europe PMC] [Abstract]
- "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
- "HspBP1, an Hsp70 cochaperone, has two structural domains and is capable of altering the conformation of the Hsp70 ATPase domain."J. Biol. Chem. 278:19017-19022(2003) [PubMed] [Europe PMC] [Abstract]
- "The cochaperone HspBP1 inhibits the CHIP ubiquitin ligase and stimulates the maturation of the cystic fibrosis transmembrane conductance regulator."Mol. Biol. Cell 15:4003-4010(2004) [PubMed] [Europe PMC] [Abstract]
- "A quantitative atlas of mitotic phosphorylation."Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
- "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
- "Initial characterization of the human central proteome."BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
- "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
- "Regulation of Hsp70 function by HspBP1: structural analysis reveals an alternate mechanism for Hsp70 nucleotide exchange."Mol. Cell 17:367-379(2005) [PubMed] [Europe PMC] [Abstract]