Heat Shock 105kDa/110kDa Protein 1 (HSPH1)

HSPH1, The deduced 858-amino acid protein contains an HSP70 -like motif and shares 93.4% identity with mouse Hsph1-alpha. Northern blot analysis detected highest expression in brain, lung, and testis. Expression was lower in heart, skeletal muscle, kidney, pancreas, spleen, ovary, small intestine, colon, and peripheral blood leukocytes, and weak in liver. No expression was detected in thymus and prostate.The HSP105-alpha and HSP105-beta proteins contain 858 and 814 amino acids, respectively, and both have an N-terminal ATP-binding domain. Northern blot analysis detected a 4-kb HSP105 transcript in HeLa cells. Immunohistochemical analysis localized HSP105 mainly in the cytoplasm. Database analysis indicated that both HSP105 isoforms are highly conserved during evolution.