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4-Hydroxyphenylpyruvate Dioxygenase (HPD)

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PPD; 4-HPPD; 4HPPD; GLOD3; Glyoxalase Domain Containing 3; 4-hydroxyphenylpyruvic acid oxidase

4-Hydroxyphenylpyruvate Dioxygenase (HPD)
CCC E251
HGNC 5147
MGI 96213
PubMed 15581571
RGD 61974
UniProt P32754
Wiki HPD
4-Hydroxyphenylpyruvate dioxygenase HPPD is an Fe-containing enzyme, that catalyzes the second reaction in the catabolism of tyrosine - the conversion of 4-hydroxyphenylpyruvate to homogentisate.The mechanism of action of 4-hydroxyphenylpyruvate dioxygenase involves a decarboxylation step and an oxidation step, followed by a rearrangement step to form homogentisic acid. The HPPD reaction occurs through a NIH shift and involves the oxidative decarboxylation of an α-oxo acid as well as aromatic ring hydroxylation. The NIH-shift, which has been demonstrated through isotope-labeling studies, involves migration of an alkyl group to form a more stable carbocation. The shift, accounts for the observation that C3 is bonded to C4 in 4-hydroxyphenylpyruvate but to C5 in homogentisate.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPE251Hu01 Recombinant 4-Hydroxyphenylpyruvate Dioxygenase (HPD) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAE251Hu01 Polyclonal Antibody to 4-Hydroxyphenylpyruvate Dioxygenase (HPD) WB; IHC; ICC; IP.
MAE251Hu21 Monoclonal Antibody to 4-Hydroxyphenylpyruvate Dioxygenase (HPD) WB; IHC; ICC/IF
MAE251Hu22 Monoclonal Antibody to 4-Hydroxyphenylpyruvate Dioxygenase (HPD) WB; IHC
MAE251Hu25 Monoclonal Antibody to 4-Hydroxyphenylpyruvate Dioxygenase (HPD) WB; IHC; ICC; IP.
MAE251Hu23 Monoclonal Antibody to 4-Hydroxyphenylpyruvate Dioxygenase (HPD) WB; IHC
MAE251Hu24 Monoclonal Antibody to 4-Hydroxyphenylpyruvate Dioxygenase (HPD) WB; IHC; ICC; IP.
MAE251Hu27 Monoclonal Antibody to 4-Hydroxyphenylpyruvate Dioxygenase (HPD) WB; IHC; ICC; IP.
MAE251Hu29 Monoclonal Antibody to 4-Hydroxyphenylpyruvate Dioxygenase (HPD) WB; IHC; ICC; IP.
Assay Kits SEE251Hu ELISA Kit for 4-Hydroxyphenylpyruvate Dioxygenase (HPD) Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant 4-Hydroxyphenylpyruvate Dioxygenase (HPD) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to 4-Hydroxyphenylpyruvate Dioxygenase (HPD) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to 4-Hydroxyphenylpyruvate Dioxygenase (HPD) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for 4-Hydroxyphenylpyruvate Dioxygenase (HPD) CLIA Kit Customized Service Offer
n/a ELISA Kit for 4-Hydroxyphenylpyruvate Dioxygenase (HPD) ELISA Kit Customized Service Offer

Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant 4-Hydroxyphenylpyruvate Dioxygenase (HPD) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to 4-Hydroxyphenylpyruvate Dioxygenase (HPD) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to 4-Hydroxyphenylpyruvate Dioxygenase (HPD) Polyclonal Antibody Customized Service Offer
Assay Kits SEE251Ra ELISA Kit for 4-Hydroxyphenylpyruvate Dioxygenase (HPD) Enzyme-linked immunosorbent assay for Antigen Detection.
  1. "Structure of the human 4-hydroxyphenylpyruvic acid dioxygenase gene (HPD)."Genomics 23:534-539(1994) [PubMed] [Europe PMC] [Abstract]
  2. "Regional assignment of the human 4-hydroxyphenylpyruvate dioxygenase gene (HPD) to 12q24-->qter by fluorescence in situ hybridization."Cytogenet. Cell Genet. 71:374-376(1995) [PubMed] [Europe PMC] [Abstract]
  3. "Human 4-hydroxyphenylpyruvate dioxygenase gene (HPD)."Genomics 44:292-299(1997) [PubMed] [Europe PMC] [Abstract]
  4. "Complete sequencing and characterization of 21,243 full-length human cDNAs." Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
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  9. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]
  10. "Crystal structure of human 4-hydroxyphenylpyruvate dioxygenase."Submitted (SEP-2009) to the PDB data bank
  11. "Mutations in the 4-hydroxyphenylpyruvate dioxygenase gene (HPD) in patients with tyrosinemia type III."Hum. Genet. 106:654-662(2000) [PubMed] [Europe PMC] [Abstract]
  12. "Mutations in the 4-hydroxyphenylpyruvic acid dioxygenase gene are responsible for tyrosinemia type III and hawkinsinuria."Mol. Genet. Metab. 71:506-510(2000) [PubMed] [Europe PMC] [Abstract]