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ERM Binding Protein 50 (EBP50)

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SLC9A3R1; NHERF; Ezrin-radixin-moesin-binding phosphoprotein 50; Solute Carrier Family 9 Member 3 Regulator 1,Sodium/Hydrogen Exchanger; Na+/H+ Exchanger Regulatory

ERM Binding Protein 50 (EBP50)
CCC E339
HGNC 11075
MGI 1349482
PubMed 12830000
RGD 708538
UniProt O14745
SLC9A3R1 encodes a sodium/hydrogen exchanger regulatory cofactor. The protein interacts with and regulates various proteins including the cystic fibrosis transmembrane conductance regulator and G-protein coupled receptors such as the beta2-adrenergic receptor and the parathyroid hormone 1 receptor. The protein also interacts with proteins that function as linkers between integral membrane and cytoskeletal proteins. The protein localizes to actin-rich structures including membrane ruffles, microvilli, and filopodia. Mutations in this gene result in hypophosphatemic nephrolithiasis/osteoporosis type 2, and loss of heterozygosity of this gene is implicated in breast cancer. Detected in liver, kidney, pancreas, prostate, spleen, small intestine and placenta, in particular in the syncytiotrophoblast.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant ERM Binding Protein 50 (EBP50) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to ERM Binding Protein 50 (EBP50) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to ERM Binding Protein 50 (EBP50) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for ERM Binding Protein 50 (EBP50) CLIA Kit Customized Service Offer
n/a ELISA Kit for ERM Binding Protein 50 (EBP50) ELISA Kit Customized Service Offer

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant ERM Binding Protein 50 (EBP50) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to ERM Binding Protein 50 (EBP50) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to ERM Binding Protein 50 (EBP50) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for ERM Binding Protein 50 (EBP50) CLIA Kit Customized Service Offer
n/a ELISA Kit for ERM Binding Protein 50 (EBP50) ELISA Kit Customized Service Offer

Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant ERM Binding Protein 50 (EBP50) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to ERM Binding Protein 50 (EBP50) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to ERM Binding Protein 50 (EBP50) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for ERM Binding Protein 50 (EBP50) CLIA Kit Customized Service Offer
n/a ELISA Kit for ERM Binding Protein 50 (EBP50) ELISA Kit Customized Service Offer
  1. "Identification of EBP50: a PDZ-containing phosphoprotein that associates with members of the ezrin-radixin-moesin family."J. Cell Biol. 139:169-179(1997) [PubMed] [Europe PMC] [Abstract]
  2. "NHE-RF, a regulatory cofactor for Na(+)-H+ exchange, is a common interactor for merlin and ERM (MERM) proteins."J. Biol. Chem. 273:1273-1276(1998) [PubMed] [Europe PMC] [Abstract]
  3. "Complete sequencing and characterization of 21,243 full-length human cDNAs." Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
  4. "DNA sequence of human chromosome 17 and analysis of rearrangement in the human lineage." Nature 440:1045-1049(2006) [PubMed] [Europe PMC] [Abstract]
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
  6. "Exploring proteomes and analyzing protein processing by mass spectrometric identification of sorted N-terminal peptides."Nat. Biotechnol. 21:566-569(2003) [PubMed] [Europe PMC] [Abstract]
  7. , Submitted (MAY-2006) to UniProtKB
  8. "cAMP-mediated inhibition of the epithelial brush border Na+/H+ exchanger, NHE3, requires an associated regulatory protein."Proc. Natl. Acad. Sci. U.S.A. 94:3010-3015(1997) [PubMed] [Europe PMC] [Abstract]
  9. "A kinase-regulated PDZ-domain interaction controls endocytic sorting of the beta2-adrenergic receptor."Nature 401:286-290(1999) [PubMed] [Europe PMC] [Abstract]
  10. "Interaction between two adapter proteins, PAG and EBP50: a possible link between membrane rafts and actin cytoskeleton."FEBS Lett. 507:133-136(2001) [PubMed] [Europe PMC] [Abstract]
  11. "Identification of EPI64, a TBC/rabGAP domain-containing microvillar protein that binds to the first PDZ domain of EBP50 and E3KARP."J. Cell Biol. 153:191-206(2001) [PubMed] [Europe PMC] [Abstract]
  12. "Protein kinase C epsilon-dependent regulation of cystic fibrosis transmembrane regulator involves binding to a receptor for activated C kinase (RACK1) and RACK1 binding to Na+/H+ exchange regulatory factor."J. Biol. Chem. 277:22925-22933(2002) [PubMed] [Europe PMC] [Abstract]
  13. "Ezrin-radixin-moesin-binding phosphoprotein-50/Na+/H+ exchanger regulatory factor (EBP50/NHERF) blocks U50,488H-induced down-regulation of the human kappa opioid receptor by enhancing its recycling rate."J. Biol. Chem. 277:27545-27552(2002) [PubMed] [Europe PMC] [Abstract]
  14. "Epithelial inducible nitric-oxide synthase is an apical EBP50-binding protein that directs vectorial nitric oxide output."J. Biol. Chem. 277:33132-33138(2002) [PubMed] [Europe PMC] [Abstract]
  15. "Regulation of GTP-binding protein alpha q (Galpha q) signaling by the ezrin-radixin-moesin-binding phosphoprotein-50 (EBP50)."J. Biol. Chem. 277:40751-40759(2002) [PubMed] [Europe PMC] [Abstract]
  16. "The cystic fibrosis transmembrane conductance regulator interacts with and regulates the activity of the HCO3- salvage transporter human Na+-HCO3-cotransport isoform 3."J. Biol. Chem. 277:50503-50509(2002) [PubMed] [Europe PMC] [Abstract]
  17. "The PDZ-interacting domain of TRPC4 controls its localization and surface expression in HEK293 cells."J. Cell Sci. 115:3497-3508(2002) [PubMed] [Europe PMC] [Abstract]
  18. "Estrogen receptor inducibility of the human Na+/H+ exchanger regulatory factor/ezrin-radixin-moesin binding protein 50 (NHE-RF/EBP50) gene involving multiple half-estrogen response elements."Mol. Endocrinol. 16:1828-1839(2002) [PubMed] [Europe PMC] [Abstract]
  19. "The COOH termini of NBC3 and the 56-kDa H+-ATPase subunit are PDZ motifs involved in their interaction."Am. J. Physiol. 284:C667-C673(2003) [PubMed] [Europe PMC] [Abstract]
  20. "Isoforms of SLC26A6 mediate anion transport and have functional PDZ interaction domains."Am. J. Physiol. 284:C769-C779(2003) [PubMed] [Europe PMC] [Abstract]
  21. "EBP50, a beta-catenin-associating protein, enhances Wnt signaling and is over-expressed in hepatocellular carcinoma."Hepatology 38:178-186(2003) [PubMed] [Europe PMC] [Abstract]
  22. "The PDZ-binding chloride channel ClC-3B localizes to the Golgi and associates with cystic fibrosis transmembrane conductance regulator-interacting PDZ proteins."J. Biol. Chem. 278:6440-6449(2003) [PubMed] [Europe PMC] [Abstract]
  23. "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract]
  24. "Improved titanium dioxide enrichment of phosphopeptides from HeLa cells and high confident phosphopeptide identification by cross-validation of MS/MS and MS/MS/MS spectra."J. Proteome Res. 6:4150-4162(2007) [PubMed] [Europe PMC] [Abstract]
  25. "Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis."J. Proteome Res. 7:1346-1351(2008) [PubMed] [Europe PMC] [Abstract]
  26. "Phosphorylation analysis of primary human T lymphocytes using sequential IMAC and titanium oxide enrichment."J. Proteome Res. 7:5167-5176(2008) [PubMed] [Europe PMC] [Abstract]
  27. "Phosphoproteome of resting human platelets."J. Proteome Res. 7:526-534(2008) [PubMed] [Europe PMC] [Abstract]
  28. "A quantitative atlas of mitotic phosphorylation."Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
  29. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
  30. "MCC, a new interacting protein for Scrib, is required for cell migration in epithelial cells."FEBS Lett. 583:2326-2332(2009) [PubMed] [Europe PMC] [Abstract]
  31. "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
  32. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
  33. "Initial characterization of the human central proteome."BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
  34. "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
  35. "Comparative large-scale characterisation of plant vs. mammal proteins reveals similar and idiosyncratic N-alpha acetylation features."Mol. Cell. Proteomics 11:M111.015131-M111.015131(2012) [PubMed] [Europe PMC] [Abstract]
  36. "A new human NHERF1 mutation decreases renal phosphate transporter NPT2a expression by a PTH-independent mechanism."PLoS ONE 7:E34764-E34764(2012) [PubMed] [Europe PMC] [Abstract]
  37. "N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]
  38. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]
  39. "Structural basis of the Na+/H+ exchanger regulatory factor PDZ1 interaction with the carboxyl-terminal region of the cystic fibrosis transmembrane conductance regulator."J. Biol. Chem. 276:19683-19686(2001) [PubMed] [Europe PMC] [Abstract]
  40. "Crystal structure of the PDZ1 domain of human Na(+)/H(+) exchanger regulatory factor provides insights into the mechanism of carboxyl-terminal leucine recognition by class I PDZ domains."J. Mol. Biol. 308:963-973(2001) [PubMed] [Europe PMC] [Abstract]
  41. "Structural determinants of the Na+/H+ exchanger regulatory factor interaction with the beta 2 adrenergic and platelet-derived growth factor receptors."J. Biol. Chem. 277:18973-18978(2002) [PubMed] [Europe PMC] [Abstract]
  42. "The EBP50-moesin interaction involves a binding site regulated by direct masking on the FERM domain."J. Cell Sci. 117:1547-1552(2004) [PubMed] [Europe PMC] [Abstract]
  43. "Structural basis for NHERF recognition by ERM proteins."Structure 14:777-789(2006) [PubMed] [Europe PMC] [Abstract]
  44. "The crystal structure of the 2nd PDZ domain of the human NHERF-1 (SLC9A3R1)."Submitted (MAR-2007) to the PDB data bank
  45. "NHERF1 mutations and responsiveness of renal parathyroid hormone."N. Engl. J. Med. 359:1128-1135(2008) [PubMed] [Europe PMC] [Abstract]