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Cyclin Dependent Kinase Inhibitor 1A (CDKN1A)

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WAF1; p21; CIP1; p21WAF1; CAP20; CDKN1; MDA-6; SDI1; p21CIP1; CDK-Interacting Protein 1; Melanoma differentiation-associated protein 6

Cyclin Dependent Kinase Inhibitor 1A (CDKN1A)
CCC E624
HGNC 1784
MGI 104556
PubMed 22634755
RGD 69328
UniProt P38936
Wiki P21
The p21 (WAF1) protein binds to and inhibits the activity of cyclin-CDK2 or -CDK4 complexes, and thus functions as a regulator of cell cycle progression at G1. The expression of this gene is tightly controlled by the tumor suppressor protein p53, through which this protein mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli.
In addition to growth arrest, p21 can mediate cellular senescence and one of the ways it was discovered was as a senescent cell-derived inhibitor. The p21(WAF1) protein can also interact with proliferating cell nuclear antigen (PCNA), a DNA polymerase accessory factor, and plays a regulatory role in S phase DNA replication and DNA damage repair.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPE624Hu01 Recombinant Cyclin Dependent Kinase Inhibitor 1A (CDKN1A) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAE624Hu01 Polyclonal Antibody to Cyclin Dependent Kinase Inhibitor 1A (CDKN1A) WB; IHC; ICC; IP.
Assay Kits SEE624Hu ELISA Kit for Cyclin Dependent Kinase Inhibitor 1A (CDKN1A) Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPE624Mu01 Recombinant Cyclin Dependent Kinase Inhibitor 1A (CDKN1A) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAE624Mu01 Polyclonal Antibody to Cyclin Dependent Kinase Inhibitor 1A (CDKN1A) WB; IHC; ICC; IP.
Assay Kits n/a CLIA Kit for Cyclin Dependent Kinase Inhibitor 1A (CDKN1A) CLIA Kit Customized Service Offer
n/a ELISA Kit for Cyclin Dependent Kinase Inhibitor 1A (CDKN1A) ELISA Kit Customized Service Offer

Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPE624Ra01 Recombinant Cyclin Dependent Kinase Inhibitor 1A (CDKN1A) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAE624Ra01 Polyclonal Antibody to Cyclin Dependent Kinase Inhibitor 1A (CDKN1A) WB; IHC; ICC; IP.
MAE624Ra21 Monoclonal Antibody to Cyclin Dependent Kinase Inhibitor 1A (CDKN1A) WB; IHC; ICC; IP.
Assay Kits SEE624Ra ELISA Kit for Cyclin Dependent Kinase Inhibitor 1A (CDKN1A) Enzyme-linked immunosorbent assay for Antigen Detection.
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  2. "WAF1, a potential mediator of p53 tumor suppression."Cell 75:817-825(1993) [PubMed] [Europe PMC] [Abstract]
  3. "p21 is a universal inhibitor of cyclin kinases."Nature 366:701-704(1993) [PubMed] [Europe PMC] [Abstract]
  4. "Use of a sensitive and efficient subtraction hybridization protocol for the identification of genes differentially regulated during the induction of differentiation in human melanoma cells."Mol. Cell. Differ. 1:285-299(1993)
  5. "The melanoma differentiation-associated gene mda-6, which encodes the cyclin-dependent kinase inhibitor p21, is differentially expressed during growth, differentiation and progression in human melanoma cells."Oncogene 10:1855-1864(1995) [PubMed] [Europe PMC] [Abstract]
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  7. "Two variants of the CIP1/WAF1 gene occur together and are associated with human cancer."Hum. Mol. Genet. 4:1089-1092(1995) [PubMed] [Europe PMC] [Abstract]
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  12. "Reversible phosphorylation at the C-terminal regulatory domain of p21(Waf1/Cip1) modulates proliferating cell nuclear antigen binding."J. Biol. Chem. 275:11529-11537(2000) [PubMed] [Europe PMC] [Abstract]
  13. "New functional activities for the p21 family of CDK inhibitors."Genes Dev. 11:847-862(1997) [PubMed] [Europe PMC] [Abstract]
  14. "A degradation signal located in the C-terminus of p21WAF1/CIP1 is a binding site for the C8 alpha-subunit of the 20S proteasome."EMBO J. 20:2367-2375(2001) [PubMed] [Europe PMC] [Abstract]
  15. "Akt-dependent phosphorylation of p21(Cip1) regulates PCNA binding and proliferation of endothelial cells."Mol. Cell. Biol. 21:5644-5657(2001) [PubMed] [Europe PMC] [Abstract]
  16. "Phosphorylation of the cell cycle inhibitor p21Cip1/WAF1 by Pim-1 kinase."Biochim. Biophys. Acta 1593:45-55(2002) [PubMed] [Europe PMC] [Abstract]
  17. "N-Terminal ubiquitination of extracellular signal-regulated kinase 3 and p21 directs their degradation by the proteasome."Mol. Cell. Biol. 24:6140-6150(2004) [PubMed] [Europe PMC] [Abstract]
  18. "Only Akt1 is required for proliferation, while Akt2 promotes cell cycle exit through p21 binding."Mol. Cell. Biol. 26:8267-8280(2006) [PubMed] [Europe PMC] [Abstract]
  19. "A probability-based approach for high-throughput protein phosphorylation analysis and site localization."Nat. Biotechnol. 24:1285-1292(2006) [PubMed] [Europe PMC] [Abstract]
  20. "PCNA-dependent regulation of p21 ubiquitylation and degradation via the CRL4Cdt2 ubiquitin ligase complex."Genes Dev. 22:2496-2506(2008) [PubMed] [Europe PMC] [Abstract]
  21. "The CRL4Cdt2 ubiquitin ligase targets the degradation of p21Cip1 to control replication licensing."Genes Dev. 22:2507-2519(2008) [PubMed] [Europe PMC] [Abstract]
  22. "CDK inhibitor p21 is degraded by a proliferating cell nuclear antigen-coupled Cul4-DDB1Cdt2 pathway during S phase and after UV irradiation."J. Biol. Chem. 283:29045-29052(2008) [PubMed] [Europe PMC] [Abstract]
  23. "A quantitative atlas of mitotic phosphorylation."Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
  24. "A dual role of Cdk2 in DNA damage response."Cell Div. 4:9-9(2009) [PubMed] [Europe PMC] [Abstract]
  25. "Differential regulation of p53 and p21 by MKRN1 E3 ligase controls cell cycle arrest and apoptosis."EMBO J. 28:2100-2113(2009) [PubMed] [Europe PMC] [Abstract]
  26. "Ionizing radiation induces ATM-independent degradation of p21Cip1 in transformed cells."J. Biol. Chem. 284:15061-15070(2009) [PubMed] [Europe PMC] [Abstract]
  27. "Pim-2 phosphorylation of p21(Cip1/WAF1) enhances its stability and inhibits cell proliferation in HCT116 cells."Int. J. Biochem. Cell Biol. 42:1030-1038(2010) [PubMed] [Europe PMC] [Abstract]
  28. "N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]
  29. "ZNF313 is a novel cell cycle activator with an E3 ligase activity inhibiting cellular senescence by destabilizing p21(WAF1.)."Cell Death Differ. 20:1055-1067(2013) [PubMed] [Europe PMC] [Abstract]
  30. "A mouse model uncovers LKB1 as an UVB-induced DNA damage sensor mediating CDKN1A (p21WAF1/CIP1) degradation."PLoS Genet. 10:E1004721-E1004721(2014) [PubMed] [Europe PMC] [Abstract]
  31. "Structure of the C-terminal region of p21(WAF1/CIP1) complexed with human PCNA."Cell 87:297-306(1996) [PubMed] [Europe PMC] [Abstract]