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Ubiquitin Conjugating Enzyme E2T (UBE2T)

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HSPC150; Cell proliferation-inducing gene 50 protein; Ubiquitin carrier protein T; Ubiquitin-protein ligase T

Ubiquitin Conjugating Enzyme E2T (UBE2T)
CCC E662
HGNC 25009
MGI 1914446
PubMed 16916645
RGD 1310816
UniProt Q9NPD8
The covalent conjugation of ubiquitin to proteins regulates diverse cellular pathways and proteins. Ubiquitin is transferred to a target protein through a concerted action of a ubiquitin-activating enzyme (E1), a ubiquitin-conjugating enzyme (E2), such as UBE2T, and a ubiquitin ligase (E3). This monoubiquitination was stimulated by the presence of FANCL and inactivated UBE2T. UBE2T activity was dependent on the conserved cys86, where a ubiquitin molecule is transferred from E1, and UBE2T underwent automonoubiquitination at lys91 in vivo. The monoubiquitination of UBE2T occurred even in the absence of an E3 in vitro.The deduced 197-amino acid protein contains a ubiquitin-conjugating enzyme active site. EST database analysis indicated widespread UBE2T expression.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Ubiquitin Conjugating Enzyme E2T (UBE2T) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Ubiquitin Conjugating Enzyme E2T (UBE2T) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Ubiquitin Conjugating Enzyme E2T (UBE2T) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Ubiquitin Conjugating Enzyme E2T (UBE2T) CLIA Kit Customized Service Offer
n/a ELISA Kit for Ubiquitin Conjugating Enzyme E2T (UBE2T) ELISA Kit Customized Service Offer

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Ubiquitin Conjugating Enzyme E2T (UBE2T) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Ubiquitin Conjugating Enzyme E2T (UBE2T) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Ubiquitin Conjugating Enzyme E2T (UBE2T) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Ubiquitin Conjugating Enzyme E2T (UBE2T) CLIA Kit Customized Service Offer
n/a ELISA Kit for Ubiquitin Conjugating Enzyme E2T (UBE2T) ELISA Kit Customized Service Offer

Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Ubiquitin Conjugating Enzyme E2T (UBE2T) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Ubiquitin Conjugating Enzyme E2T (UBE2T) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Ubiquitin Conjugating Enzyme E2T (UBE2T) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Ubiquitin Conjugating Enzyme E2T (UBE2T) CLIA Kit Customized Service Offer
n/a ELISA Kit for Ubiquitin Conjugating Enzyme E2T (UBE2T) ELISA Kit Customized Service Offer
  1. "Gene expression profiling in the human hypothalamus-pituitary-adrenal axis and full-length cDNA cloning." Proc. Natl. Acad. Sci. U.S.A. 97:9543-9548(2000) [PubMed] [Europe PMC] [Abstract]
  2. "Cloning and functional analysis of cDNAs with open reading frames for 300 previously undefined genes expressed in CD34+ hematopoietic stem/progenitor cells." Genome Res. 10:1546-1560(2000) [PubMed] [Europe PMC] [Abstract]
  3. "Complete sequencing and characterization of 21,243 full-length human cDNAs." Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
  4. "The DNA sequence and biological annotation of human chromosome 1." Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract]
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
  6. "UBE2T is the E2 in the Fanconi anemia pathway and undergoes negative autoregulation."Mol. Cell 23:589-596(2006) [PubMed] [Europe PMC] [Abstract]
  7. "UBE2T, the Fanconi anemia core complex, and FANCD2 are recruited independently to chromatin: a basis for the regulation of FANCD2 monoubiquitination."Mol. Cell. Biol. 27:8421-8430(2007) [PubMed] [Europe PMC] [Abstract]
  8. "Mechanistic insight into site-restricted monoubiquitination of FANCD2 by Ube2t, FANCL, and FANCI."Mol. Cell 32:767-777(2008) [PubMed] [Europe PMC] [Abstract]
  9. "A quantitative atlas of mitotic phosphorylation."Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
  10. "Ubiquitination and downregulation of BRCA1 by ubiquitin-conjugating enzyme E2T overexpression in human breast cancer cells."Cancer Res. 69:8752-8760(2009) [PubMed] [Europe PMC] [Abstract]
  11. "FANCI binds branched DNA and is monoubiquitinated by UBE2T-FANCL."J. Biol. Chem. 284:23182-23186(2009) [PubMed] [Europe PMC] [Abstract]
  12. "The E2 ubiquitin-conjugating enzymes direct polyubiquitination to preferred lysines."J. Biol. Chem. 285:8595-8604(2010) [PubMed] [Europe PMC] [Abstract]
  13. "Initial characterization of the human central proteome."BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
  14. "Structural analysis of human FANCL, the E3 ligase in the Fanconi anemia pathway."J. Biol. Chem. 286:32628-32637(2011) [PubMed] [Europe PMC] [Abstract]
  15. "Hypoxia disrupts the Fanconi anemia pathway and sensitizes cells to chemotherapy through regulation of UBE2T."Radiother. Oncol. 101:190-197(2011) [PubMed] [Europe PMC] [Abstract]
  16. "Ubiquitin-conjugating enzyme HSPC150."Submitted (FEB-2005) to the PDB data bank