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Sirtuin 1 (SIRT1)

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SIR2L1; Silent Mating Type Information Regulation 2 Homolog 1; NAD-Dependent Deacetylase Sirtuin-1; SIR2-like protein 1; SirtT1 75 kDa fragment

Sirtuin 1 (SIRT1)
CCC E912
HGNC 14929
MGI 2135607
PubMed 10381378
RGD 1308542
UniProt Q96EB6
Wiki Sirtuin_1
Sirtuin 1 is a member of the sirtuin family of proteins, homologs of the Sir2 gene in S. cerevisiae. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class I of the sirtuin family.
Sirtuin 1 is downregulated in cells that have high insulin resistance and inducing its expression increases insulin sensitivity, suggesting the molecule is associated with improving insulin sensitivity.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPE912Hu01 Recombinant Sirtuin 1 (SIRT1) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAE912Hu01 Polyclonal Antibody to Sirtuin 1 (SIRT1) WB; IHC; ICC; IP.
MAE912Hu22 Monoclonal Antibody to Sirtuin 1 (SIRT1) WB; IHC; ICC; IP.
LAE912Hu71 Biotin-Linked Polyclonal Antibody to Sirtuin 1 (SIRT1) WB; IHC; ICC.
LAE912Hu81 FITC-Linked Polyclonal Antibody to Sirtuin 1 (SIRT1) WB; IHC; ICC; IF.
MAE912Hu21 Monoclonal Antibody to Sirtuin 1 (SIRT1) WB; IHC; ICC; IP.
MAE912Hu23 Monoclonal Antibody to Sirtuin 1 (SIRT1) WB; IHC; ICC; IP.
Assay Kits SEE912Hu ELISA Kit for Sirtuin 1 (SIRT1) Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPE912Mu01 Recombinant Sirtuin 1 (SIRT1) Positive Control; Immunogen; SDS-PAGE; WB.
RPE912Mu02 Recombinant Sirtuin 1 (SIRT1) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAE912Mu01 Polyclonal Antibody to Sirtuin 1 (SIRT1) WB; IHC; ICC; IP.
LAE912Mu71 Biotin-Linked Polyclonal Antibody to Sirtuin 1 (SIRT1) WB; IHC; ICC.
PAE912Mu02 Polyclonal Antibody to Sirtuin 1 (SIRT1) WB; IHC; ICC; IP.
Assay Kits SEE912Mu ELISA Kit for Sirtuin 1 (SIRT1) Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPE912Ra01 Recombinant Sirtuin 1 (SIRT1) Positive Control; Immunogen; SDS-PAGE; WB.
RPE912Ra02 Recombinant Sirtuin 1 (SIRT1) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAE912Ra01 Polyclonal Antibody to Sirtuin 1 (SIRT1) WB; IHC; ICC; IP.
MAE912Ra21 Monoclonal Antibody to Sirtuin 1 (SIRT1) WB; IHC; ICC; IP.
PAE912Ra02 Polyclonal Antibody to Sirtuin 1 (SIRT1) WB; IHC; ICC; IP.
Assay Kits SEE912Ra ELISA Kit for Sirtuin 1 (SIRT1) Enzyme-linked immunosorbent assay for Antigen Detection.
SCE912Ra CLIA Kit for Sirtuin 1 (SIRT1) Chemiluminescent immunoassay for Antigen Detection.
  1. "Characterization of five human cDNAs with homology to the yeast SIR2 gene: Sir2-like proteins (sirtuins) metabolize NAD and may have protein ADP-ribosyltransferase activity."Biochem. Biophys. Res. Commun. 260:273-279(1999) [PubMed] [Europe PMC] [Abstract]
  2. "Human Sir2-related protein SIRT1 associates with the bHLH repressors HES1 and HEY2 and is involved in HES1- and HEY2-mediated transcriptional repression."Biochem. Biophys. Res. Commun. 301:250-257(2003) [PubMed] [Europe PMC] [Abstract]
  3. "The DNA sequence and comparative analysis of human chromosome 10." Nature 429:375-381(2004) [PubMed] [Europe PMC] [Abstract]
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
  5. "hSIR2(SIRT1) functions as an NAD-dependent p53 deacetylase."Cell 107:149-159(2001) [PubMed] [Europe PMC] [Abstract]
  6. "Human SIR2 deacetylates p53 and antagonizes PML/p53-induced cellular senescence."EMBO J. 21:2383-2396(2002) [PubMed] [Europe PMC] [Abstract]
  7. "Inhibition of silencing and accelerated aging by nicotinamide, a putative negative regulator of yeast sir2 and human SIRT1."J. Biol. Chem. 277:45099-45107(2002) [PubMed] [Europe PMC] [Abstract]
  8. "Small molecule activators of sirtuins extend Saccharomyces cerevisiae lifespan."Nature 425:191-196(2003) [PubMed] [Europe PMC] [Abstract]
  9. "Modulation of NF-kappaB-dependent transcription and cell survival by the SIRT1 deacetylase."EMBO J. 23:2369-2380(2004) [PubMed] [Europe PMC] [Abstract]
  10. "Mammalian SIRT1 represses forkhead transcription factors."Cell 116:551-563(2004) [PubMed] [Europe PMC] [Abstract]
  11. "FOXO4 is acetylated upon peroxide stress and deacetylated by the longevity protein hSir2(SIRT1)."J. Biol. Chem. 279:28873-28879(2004) [PubMed] [Europe PMC] [Abstract]
  12. "Human SirT1 interacts with histone H1 and promotes formation of facultative heterochromatin."Mol. Cell 16:93-105(2004) [PubMed] [Europe PMC] [Abstract]
  13. "Stress-dependent regulation of FOXO transcription factors by the SIRT1 deacetylase."Science 303:2011-2015(2004) [PubMed] [Europe PMC] [Abstract]
  14. "Calorie restriction promotes mammalian cell survival by inducing the SIRT1 deacetylase."Science 305:390-392(2004) [PubMed] [Europe PMC] [Abstract]
  15. "Suppression of FOXO1 activity by FHL2 through SIRT1-mediated deacetylation."EMBO J. 24:1021-1032(2005) [PubMed] [Europe PMC] [Abstract]
  16. "Evolutionarily conserved and nonconserved cellular localizations and functions of human SIRT proteins."Mol. Biol. Cell 16:4623-4635(2005) [PubMed] [Europe PMC] [Abstract]
  17. "Regulation of MEF2 by histone deacetylase 4- and SIRT1 deacetylase-mediated lysine modifications."Mol. Cell. Biol. 25:8456-8464(2005) [PubMed] [Europe PMC] [Abstract]
  18. "SIRT1 regulates HIV transcription via Tat deacetylation."PLoS Biol. 3:210-220(2005) [PubMed] [Europe PMC] [Abstract]
  19. "Composition and histone substrates of polycomb repressive group complexes change during cellular differentiation."Proc. Natl. Acad. Sci. U.S.A. 102:1859-1864(2005) [PubMed] [Europe PMC] [Abstract]
  20. "A probability-based approach for high-throughput protein phosphorylation analysis and site localization."Nat. Biotechnol. 24:1285-1292(2006) [PubMed] [Europe PMC] [Abstract]
  21. "Interactions between E2F1 and SirT1 regulate apoptotic response to DNA damage."Nat. Cell Biol. 8:1025-1031(2006) [PubMed] [Europe PMC] [Abstract]
  22. "Deacetylation of the retinoblastoma tumour suppressor protein by SIRT1."Biochem. J. 407:451-460(2007) [PubMed] [Europe PMC] [Abstract]
  23. "Sirt1 interacts with transducin-like enhancer of split-1 to inhibit nuclear factor kappaB-mediated transcription."Biochem. J. 408:105-111(2007) [PubMed] [Europe PMC] [Abstract]
  24. "Distinct C/EBPalpha motifs regulate lipogenic and gluconeogenic gene expression in vivo."EMBO J. 26:1081-1093(2007) [PubMed] [Europe PMC] [Abstract]
  25. "SIRT1 promotes DNA repair activity and deacetylation of Ku70."Exp. Mol. Med. 39:8-13(2007) [PubMed] [Europe PMC] [Abstract]
  26. "SIRT1 interacts with p73 and suppresses p73-dependent transcriptional activity."J. Cell. Physiol. 210:161-166(2007) [PubMed] [Europe PMC] [Abstract]
  27. "Sirtuin 1 is required for antagonist-induced transcriptional repression of androgen-responsive genes by the androgen receptor."Mol. Endocrinol. 21:1807-1821(2007) [PubMed] [Europe PMC] [Abstract]
  28. "Active regulator of SIRT1 cooperates with SIRT1 and facilitates suppression of p53 activity."Mol. Cell 28:277-290(2007) [PubMed] [Europe PMC] [Abstract]
  29. ErratumMol. Cell 28:513-513(2007)
  30. "SIRT1 deacetylates and positively regulates the nuclear receptor LXR."Mol. Cell 28:91-106(2007) [PubMed] [Europe PMC] [Abstract]
  31. "SIRT1 regulates the function of the Nijmegen breakage syndrome protein."Mol. Cell 27:149-162(2007) [PubMed] [Europe PMC] [Abstract]
  32. "An acetylation/deacetylation-SUMOylation switch through a phylogenetically conserved psiKXEP motif in the tumor suppressor HIC1 regulates transcriptional repression activity."Mol. Cell. Biol. 27:2661-2675(2007) [PubMed] [Europe PMC] [Abstract]
  33. "SIRT1 regulates the histone methyl-transferase SUV39H1 during heterochromatin formation."Nature 450:440-444(2007) [PubMed] [Europe PMC] [Abstract]
  34. "SIRT1 regulates circadian clock gene expression through PER2 deacetylation."Cell 134:317-328(2008) [PubMed] [Europe PMC] [Abstract]
  35. "Epigenetic control of rDNA loci in response to intracellular energy status."Cell 133:627-639(2008) [PubMed] [Europe PMC] [Abstract]
  36. "JNK2-dependent regulation of SIRT1 protein stability."Cell Cycle 7:3091-3097(2008) [PubMed] [Europe PMC] [Abstract]
  37. "Human immunodeficiency virus type 1 Tat protein inhibits the SIRT1 deacetylase and induces T cell hyperactivation."Cell Host Microbe 3:158-167(2008) [PubMed] [Europe PMC] [Abstract]
  38. "Regulation of WRN protein cellular localization and enzymatic activities by SIRT1-mediated deacetylation."J. Biol. Chem. 283:7590-7598(2008) [PubMed] [Europe PMC] [Abstract]
  39. "SIRT1 modulation of the acetylation status, cytosolic localization, and activity of LKB1. Possible role in AMP-activated protein kinase activation."J. Biol. Chem. 283:27628-27635(2008) [PubMed] [Europe PMC] [Abstract]
  40. "DBC1 is a negative regulator of SIRT1."Nature 451:583-586(2008) [PubMed] [Europe PMC] [Abstract]
  41. "Negative regulation of the deacetylase SIRT1 by DBC1."Nature 451:587-590(2008) [PubMed] [Europe PMC] [Abstract]
  42. "Phosphorylation regulates SIRT1 function."PLoS ONE 3:E4020-E4020(2008) [PubMed] [Europe PMC] [Abstract]
  43. "A quantitative atlas of mitotic phosphorylation."Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
  44. "A role for the NAD-dependent deacetylase Sirt1 in the regulation of autophagy."Proc. Natl. Acad. Sci. U.S.A. 105:3374-3379(2008) [PubMed] [Europe PMC] [Abstract]
  45. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
  46. "Carboxy-terminal phosphorylation of SIRT1 by protein kinase CK2."Biochem. Biophys. Res. Commun. 381:372-377(2009) [PubMed] [Europe PMC] [Abstract]
  47. "Investigating the ADP-ribosyltransferase activity of sirtuins with NAD analogues and 32P-NAD."Biochemistry 48:2878-2890(2009) [PubMed] [Europe PMC] [Abstract]
  48. "Hepatocyte-specific deletion of SIRT1 alters fatty acid metabolism and results in hepatic steatosis and inflammation."Cell Metab. 9:327-338(2009) [PubMed] [Europe PMC] [Abstract]
  49. "Transcriptional corepressor SMILE recruits SIRT1 to inhibit nuclear receptor estrogen receptor-related receptor gamma transactivation."J. Biol. Chem. 284:28762-28774(2009) [PubMed] [Europe PMC] [Abstract]
  50. "A c-Myc-SIRT1 feedback loop regulates cell growth and transformation."J. Cell Biol. 185:203-211(2009) [PubMed] [Europe PMC] [Abstract]
  51. "hSirT1-dependent regulation of the PCAF-E2F1-p73 apoptotic pathway in response to DNA damage."Mol. Cell. Biol. 29:1989-1998(2009) [PubMed] [Europe PMC] [Abstract]
  52. "JNK1 phosphorylates SIRT1 and promotes its enzymatic activity."PLoS ONE 4:E8414-E8414(2009) [PubMed] [Europe PMC] [Abstract]
  53. "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
  54. "SIRT1 promotes proliferation and prevents senescence through targeting LKB1 in primary porcine aortic endothelial cells."Circ. Res. 106:1384-1393(2010) [PubMed] [Europe PMC] [Abstract]
  55. "Role of SIRT1 in homologous recombination."DNA Repair 9:383-393(2010) [PubMed] [Europe PMC] [Abstract]
  56. "SIRT1 suppresses activator protein-1 transcriptional activity and cyclooxygenase-2 expression in macrophages."J. Biol. Chem. 285:7097-7110(2010) [PubMed] [Europe PMC] [Abstract]
  57. "SIRT1 regulates autoacetylation and histone acetyltransferase activity of TIP60."J. Biol. Chem. 285:11458-11464(2010) [PubMed] [Europe PMC] [Abstract]
  58. "SIRT1 deacetylates and inhibits SREBP-1C activity in regulation of hepatic lipid metabolism."J. Biol. Chem. 285:33959-33970(2010) [PubMed] [Europe PMC] [Abstract]
  59. "Sirtuin 1 modulates cellular responses to hypoxia by deacetylating hypoxia-inducible factor 1alpha."Mol. Cell 38:864-878(2010) [PubMed] [Europe PMC] [Abstract]
  60. "SIRT1 regulates UV-induced DNA repair through deacetylating XPA."Mol. Cell 39:247-258(2010) [PubMed] [Europe PMC] [Abstract]
  61. "SIRT1 deacetylates APE1 and regulates cellular base excision repair."Nucleic Acids Res. 38:832-845(2010) [PubMed] [Europe PMC] [Abstract]
  62. "Transcriptional corepressor SHP recruits SIRT1 histone deacetylase to inhibit LRH-1 transactivation."Nucleic Acids Res. 38:4607-4619(2010) [PubMed] [Europe PMC] [Abstract]
  63. "SIRT1 negatively regulates the mammalian target of rapamycin."PLoS ONE 5:E9199-E9199(2010) [PubMed] [Europe PMC] [Abstract]
  64. "SIRT1 undergoes alternative splicing in a novel auto-regulatory loop with p53."PLoS ONE 5:E13502-E13502(2010) [PubMed] [Europe PMC] [Abstract]
  65. "Regulation of global genome nucleotide excision repair by SIRT1 through xeroderma pigmentosum C."Proc. Natl. Acad. Sci. U.S.A. 107:22623-22628(2010) [PubMed] [Europe PMC] [Abstract]
  66. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
  67. "SIRT1 and SIRT3 deacetylate homologous substrates: AceCS1,2 and HMGCS1,2."Aging (Albany NY) 3:635-642(2011) [PubMed] [Europe PMC] [Abstract]
  68. "Tumor necrosis factor alpha-mediated cleavage and inactivation of SirT1 in human osteoarthritic chondrocytes."Arthritis Rheum. 63:2363-2373(2011) [PubMed] [Europe PMC] [Abstract]
  69. "Regulation of unfolded protein response modulator XBP1s by acetylation and deacetylation."Biochem. J. 433:245-252(2011) [PubMed] [Europe PMC] [Abstract]
  70. "Initial characterization of the human central proteome."BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
  71. "EVI1 up-regulates the stress responsive gene SIRT1 which triggers deacetylation and degradation of EVI1."Biochim. Biophys. Acta 1809:269-275(2011) [PubMed] [Europe PMC] [Abstract]
  72. "Energy sensing factors PGC-1alpha and SIRT1 modulate PXR expression and function."Biochem. Pharmacol. 82:2008-2015(2011) [PubMed] [Europe PMC] [Abstract]
  73. "Sirt1 deacetylates c-Myc and promotes c-Myc/Max association."Int. J. Biochem. Cell Biol. 43:1573-1581(2011) [PubMed] [Europe PMC] [Abstract]
  74. "MST1 promotes apoptosis through regulating Sirt1-dependent p53 deacetylation."J. Biol. Chem. 286:6940-6945(2011) [PubMed] [Europe PMC] [Abstract]
  75. "Cancer cell survival following DNA damage-mediated premature senescence is regulated by mammalian target of rapamycin (mTOR)-dependent Inhibition of sirtuin 1."J. Biol. Chem. 286:19100-19108(2011) [PubMed] [Europe PMC] [Abstract]
  76. "Stabilization of Suv39H1 by SirT1 is part of oxidative stress response and ensures genome protection."Mol. Cell 42:210-223(2011) [PubMed] [Europe PMC] [Abstract]
  77. "SIRT1 deacetylates the DNA methyltransferase 1 (DNMT1) protein and alters its activities."Mol. Cell. Biol. 31:4720-4734(2011) [PubMed] [Europe PMC] [Abstract]
  78. "SIRT1 promotes N-Myc oncogenesis through a positive feedback loop involving the effects of MKP3 and ERK on N-Myc protein stability."PLoS Genet. 7:E1002135-E1002135(2011) [PubMed] [Europe PMC] [Abstract]
  79. "The evolutionarily conserved longevity determinants HCF-1 and SIR-2.1/SIRT1 collaborate to regulate DAF-16/FOXO."PLoS Genet. 7:E1002235-E1002235(2011) [PubMed] [Europe PMC] [Abstract]
  80. "Methyltransferase Set7/9 regulates p53 activity by interacting with Sirtuin 1 (SIRT1)."Proc. Natl. Acad. Sci. U.S.A. 108:1925-1930(2011) [PubMed] [Europe PMC] [Abstract]
  81. "The deacetylase SIRT1 promotes membrane localization and activation of Akt and PDK1 during tumorigenesis and cardiac hypertrophy."Sci. Signal. 4:RA46-RA46(2011) [PubMed] [Europe PMC] [Abstract]
  82. "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
  83. "75kDa SirT1 blocks TNFalpha-mediated apoptosis in human osteoarthritic chondrocytes."Arthritis Rheum. 64:718-728(2012) [PubMed] [Europe PMC] [Abstract]
  84. "SIRT1 links CIITA deacetylation to MHC II activation."Nucleic Acids Res. 39:9549-9558(2011) [PubMed] [Europe PMC] [Abstract]
  85. "PML regulates PER2 nuclear localization and circadian function."EMBO J. 31:1427-1439(2012) [PubMed] [Europe PMC] [Abstract]
  86. "Comparative large-scale characterisation of plant vs. mammal proteins reveals similar and idiosyncratic N-alpha acetylation features."Mol. Cell. Proteomics 11:M111.015131-M111.015131(2012) [PubMed] [Europe PMC] [Abstract]
  87. "Deacetylation of FOXO3 by SIRT1 or SIRT2 leads to Skp2-mediated FOXO3 ubiquitination and degradation."Oncogene 31:1546-1557(2012) [PubMed] [Europe PMC] [Abstract]
  88. "N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]
  89. "Deleted in breast cancer 1 (DBC1) deficiency results in apoptosis of breast cancer cells through impaired responses to UV-induced DNA damage."Cancer Lett. 333:180-186(2013) [PubMed] [Europe PMC] [Abstract]
  90. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]