≡

Ring Finger Protein 8 (RNF8)

[Edit]

E3 ubiquitin-protein ligase RNF8

Ring Finger Protein 8 (RNF8)
CCC E957
HGNC 10071
MGI 1929069
PubMed 21558560
RGD 1308035
UniProt O76064
Wiki RNF8
E3 ubiquitin-protein ligase RNF8 contains a RING finger motif and an FHA domain. This protein has been shown to interact with several class II ubiquitin-conjugating enzymes (E2), including UBE2E1/UBCH6, UBE2E2, and UBE2E3, and may act as a ubiquitin ligase (E3) in the ubiquitination of certain nuclear proteins. Alternatively spliced transcript variants encoding distinct isoforms have been reported. The deduced 485-amino acid protein contains a C3HC4-type zinc finger signature, suggesting that it is involved in nucleic acid management. RT-PCR analysis detected moderate RNF8 expression in brain, testis, and ovary, with little to no expression in all other tissues examined. In vitro translation resulted in a protein with an apparent molecular mass of 62 kD by SDS-PAGE.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Ring Finger Protein 8 (RNF8) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Ring Finger Protein 8 (RNF8) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Ring Finger Protein 8 (RNF8) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Ring Finger Protein 8 (RNF8) CLIA Kit Customized Service Offer
n/a ELISA Kit for Ring Finger Protein 8 (RNF8) ELISA Kit Customized Service Offer

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Ring Finger Protein 8 (RNF8) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Ring Finger Protein 8 (RNF8) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Ring Finger Protein 8 (RNF8) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Ring Finger Protein 8 (RNF8) CLIA Kit Customized Service Offer
n/a ELISA Kit for Ring Finger Protein 8 (RNF8) ELISA Kit Customized Service Offer

Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Ring Finger Protein 8 (RNF8) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Ring Finger Protein 8 (RNF8) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Ring Finger Protein 8 (RNF8) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Ring Finger Protein 8 (RNF8) CLIA Kit Customized Service Offer
n/a ELISA Kit for Ring Finger Protein 8 (RNF8) ELISA Kit Customized Service Offer
  1. "Isolation, tissue expression, and chromosomal assignment of a novel human gene which encodes a protein with RING finger motif."J. Hum. Genet. 43:272-274(1998) [PubMed] [Europe PMC] [Abstract]
  2. "The RING finger protein RNF8 recruits UBC13 for lysine 63-based self polyubiquitylation."J. Cell. Biochem. 97:572-582(2006) [PubMed] [Europe PMC] [Abstract]
  3. "Prediction of the coding sequences of unidentified human genes. X. The complete sequences of 100 new cDNA clones from brain which can code for large proteins in vitro."DNA Res. 5:169-176(1998) [PubMed] [Europe PMC] [Abstract]
  4. "Complete sequencing and characterization of 21,243 full-length human cDNAs." Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
  5. "The DNA sequence and analysis of human chromosome 6." Nature 425:805-811(2003) [PubMed] [Europe PMC] [Abstract]
  6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
  7. "N-terminally extended human ubiquitin-conjugating enzymes (E2s) mediate the ubiquitination of RING-finger proteins, ARA54 and RNF8."Eur. J. Biochem. 268:2725-2732(2001) [PubMed] [Europe PMC] [Abstract]
  8. "The RING finger protein, RNF8, interacts with retinoid X receptor alpha and enhances its transcription-stimulating activity."J. Biol. Chem. 279:18926-18934(2004) [PubMed] [Europe PMC] [Abstract]
  9. "RNF8 ubiquitylates histones at DNA double-strand breaks and promotes assembly of repair proteins."Cell 131:887-900(2007) [PubMed] [Europe PMC] [Abstract]
  10. "Ubc13/Rnf8 ubiquitin ligases control foci formation of the Rap80/Abraxas/Brca1/Brcc36 complex in response to DNA damage."Proc. Natl. Acad. Sci. U.S.A. 104:20759-20763(2007) [PubMed] [Europe PMC] [Abstract]
  11. "Orchestration of the DNA-damage response by the RNF8 ubiquitin ligase."Science 318:1637-1640(2007) [PubMed] [Europe PMC] [Abstract]
  12. "Regulation of mitotic exit by the RNF8 ubiquitin ligase."Oncogene 27:1355-1365(2008) [PubMed] [Europe PMC] [Abstract]
  13. "PCNA is ubiquitinated by RNF8."Cell Cycle 7:3399-3404(2008) [PubMed] [Europe PMC] [Abstract]
  14. "RNF8-dependent and RNF8-independent regulation of 53BP1 in response to DNA damage."J. Biol. Chem. 283:13549-13555(2008) [PubMed] [Europe PMC] [Abstract]
  15. "Noncanonical E2 variant-independent function of UBC13 in promoting checkpoint protein assembly."Mol. Cell. Biol. 28:6104-6112(2008) [PubMed] [Europe PMC] [Abstract]
  16. "A quantitative atlas of mitotic phosphorylation."Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
  17. "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
  18. "HERC2 coordinates ubiquitin-dependent assembly of DNA repair factors on damaged chromosomes."Nat. Cell Biol. 12:80-86(2010) [PubMed] [Europe PMC] [Abstract]
  19. "The RIDDLE syndrome protein mediates a ubiquitin-dependent signaling cascade at sites of DNA damage."Cell 136:420-434(2009) [PubMed] [Europe PMC] [Abstract]
  20. "RNF168 binds and amplifies ubiquitin conjugates on damaged chromosomes to allow accumulation of repair proteins."Cell 136:435-446(2009) [PubMed] [Europe PMC] [Abstract]
  21. "Accumulation of Pax2 transactivation domain interaction protein (PTIP) at sites of DNA breaks via RNF8-dependent pathway is required for cell survival after DNA damage."J. Biol. Chem. 284:7284-7293(2009) [PubMed] [Europe PMC] [Abstract]
  22. "Histone ubiquitination associates with BRCA1-dependent DNA damage response."Mol. Cell. Biol. 29:849-860(2009) [PubMed] [Europe PMC] [Abstract]
  23. "The Rap80-BRCC36 de-ubiquitinating enzyme complex antagonizes RNF8-Ubc13-dependent ubiquitination events at DNA double strand breaks."Proc. Natl. Acad. Sci. U.S.A. 106:3166-3171(2009) [PubMed] [Europe PMC] [Abstract]
  24. "ATM-dependent chromatin changes silence transcription in cis to DNA double-strand breaks."Cell 141:970-981(2010) [PubMed] [Europe PMC] [Abstract]
  25. "Critical roles of ring finger protein RNF8 in replication stress responses."J. Biol. Chem. 286:22355-22361(2011) [PubMed] [Europe PMC] [Abstract]
  26. "DNA-damage response and repair activities at uncapped telomeres depend on RNF8."Nat. Cell Biol. 13:1139-1145(2011) [PubMed] [Europe PMC] [Abstract]
  27. "RNF8 regulates assembly of RAD51 at DNA double-strand breaks in the absence of BRCA1 and 53BP1."Cancer Res. 72:4974-4983(2012) [PubMed] [Europe PMC] [Abstract]
  28. "RNF8- and RNF168-dependent degradation of KDM4A/JMJD2A triggers 53BP1 recruitment to DNA damage sites."EMBO J. 31:1865-1878(2012) [PubMed] [Europe PMC] [Abstract]
  29. "A new non-catalytic role for ubiquitin ligase RNF8 in unfolding higher-order chromatin structure."EMBO J. 31:2511-2527(2012) [PubMed] [Europe PMC] [Abstract]
  30. "Viral E3 ubiquitin ligase-mediated degradation of a cellular E3: viral mimicry of a cellular phosphorylation mark targets the RNF8 FHA domain."Mol. Cell 46:79-90(2012) [PubMed] [Europe PMC] [Abstract]
  31. "A ubiquitin-binding protein, FAAP20, links RNF8-mediated ubiquitination to the Fanconi anemia DNA repair network."Mol. Cell 47:61-75(2012) [PubMed] [Europe PMC] [Abstract]
  32. "The E3 ligase RNF8 regulates KU80 removal and NHEJ repair."Nat. Struct. Mol. Biol. 19:201-206(2012) [PubMed] [Europe PMC] [Abstract]
  33. "Differential regulation of RNF8-mediated Lys48- and Lys63-based poly-ubiquitylation."Nucleic Acids Res. 40:196-205(2012) [PubMed] [Europe PMC] [Abstract]
  34. "Solution structure of the FHA domain of human ubiquitin ligase protein RNF8."Submitted (NOV-2005) to the PDB data bank
  35. "RNF8 transduces the DNA-damage signal via histone ubiquitylation and checkpoint protein assembly."Cell 131:901-914(2007) [PubMed] [Europe PMC] [Abstract]
  36. "RNF168 ubiquitinates K13-15 on H2A/H2AX to drive DNA Damage signaling."Cell 150:1182-1195(2012) [PubMed] [Europe PMC] [Abstract]
  37. "Molecular insights into the function of RING Finger (RNF)-containing proteins hRNF8 and hRNF168 in Ubc13/Mms2-dependent ubiquitylation."J. Biol. Chem. 287:23900-23910(2012) [PubMed] [Europe PMC] [Abstract]