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Eukaryotic Translation Initiation Factor 3B (EIF3B)

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EIF3-ETA; EIF3-P110; EIF3-P116; PRT1; eIF3b; EIF3S9; Eukaryotic translation initiation factor 3 subunit 9

Eukaryotic Translation Initiation Factor 3B (EIF3B)
CCC E998
HGNC 3280
MGI 106478
PubMed 23575475
RGD 1309018
UniProt P55884
Wiki EIF3B
Eukaryotic initiation factor-3 (eIF3) is the largest of the eIFs and consists of at least 10 nonidentical subunits in mammals. See p66 (EIF3S7). The S. cerevisiae Prt1, or p90, protein is an integral subunit of eIF3. Mutations in Prt1 impair translation initiation at 37 degrees Celsius. The predicted 873-amino acid human PRT1 protein is 31% identical to yeast Prt1. PRT1 is an acidic protein, with a predicted pI of 4.8, and contains an RNA recognition motif (RRM). Immunologic experiments demonstrated that PRT1 corresponds to the 115-kD polypeptide of eIF3 and is part of the eIF3 complex. PRT1 interacts directly with the p170 subunit of eIF3, and the region required for this association is located within the RRM. Northern blot analysis revealed that PRT1 is expressed as a 3.1-kb mRNA in HeLa cells.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Eukaryotic Translation Initiation Factor 3B (EIF3B) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Eukaryotic Translation Initiation Factor 3B (EIF3B) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Eukaryotic Translation Initiation Factor 3B (EIF3B) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Eukaryotic Translation Initiation Factor 3B (EIF3B) CLIA Kit Customized Service Offer
n/a ELISA Kit for Eukaryotic Translation Initiation Factor 3B (EIF3B) ELISA Kit Customized Service Offer

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Eukaryotic Translation Initiation Factor 3B (EIF3B) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Eukaryotic Translation Initiation Factor 3B (EIF3B) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Eukaryotic Translation Initiation Factor 3B (EIF3B) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Eukaryotic Translation Initiation Factor 3B (EIF3B) CLIA Kit Customized Service Offer
n/a ELISA Kit for Eukaryotic Translation Initiation Factor 3B (EIF3B) ELISA Kit Customized Service Offer

Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Eukaryotic Translation Initiation Factor 3B (EIF3B) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Eukaryotic Translation Initiation Factor 3B (EIF3B) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Eukaryotic Translation Initiation Factor 3B (EIF3B) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Eukaryotic Translation Initiation Factor 3B (EIF3B) CLIA Kit Customized Service Offer
n/a ELISA Kit for Eukaryotic Translation Initiation Factor 3B (EIF3B) ELISA Kit Customized Service Offer
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  2. "The human homologue of the yeast Prt1 protein is an integral part of the eukaryotic initiation factor 3 complex and interacts with p170."J. Biol. Chem. 272:1110-1116(1997) [PubMed] [Europe PMC] [Abstract]
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  7. "Molecular interaction between human tumor marker protein p150, the largest subunit of eIF3, and intermediate filament protein K7."J. Cell. Biochem. 80:483-490(2001) [PubMed] [Europe PMC] [Abstract]
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  10. "mTOR and S6K1 mediate assembly of the translation preinitiation complex through dynamic protein interchange and ordered phosphorylation events."Cell 123:569-580(2005) [PubMed] [Europe PMC] [Abstract]
  11. "Binding of eukaryotic initiation factor 3 to ribosomal 40S subunits and its role in ribosomal dissociation and anti-association."RNA 11:470-486(2005) [PubMed] [Europe PMC] [Abstract]
  12. "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract]
  13. "Translation initiation factor eIF4G-1 binds to eIF3 through the eIF3e subunit."J. Biol. Chem. 281:22917-22932(2006) [PubMed] [Europe PMC] [Abstract]
  14. "Reconstitution reveals the functional core of mammalian eIF3."EMBO J. 26:3373-3383(2007) [PubMed] [Europe PMC] [Abstract]
  15. "Human INT6/eIF3e is required for nonsense-mediated mRNA decay."EMBO Rep. 8:596-602(2007) [PubMed] [Europe PMC] [Abstract]
  16. "Structural characterization of the human eukaryotic initiation factor 3 protein complex by mass spectrometry."Mol. Cell. Proteomics 6:1135-1146(2007) [PubMed] [Europe PMC] [Abstract]
  17. "Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis."J. Proteome Res. 7:1346-1351(2008) [PubMed] [Europe PMC] [Abstract]
  18. "A quantitative atlas of mitotic phosphorylation."Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
  19. "Mass spectrometry reveals modularity and a complete subunit interaction map of the eukaryotic translation factor eIF3."Proc. Natl. Acad. Sci. U.S.A. 105:18139-18144(2008) [PubMed] [Europe PMC] [Abstract]
  20. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
  21. "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
  22. "Lysine acetylation targets protein complexes and co-regulates major cellular functions."Science 325:834-840(2009) [PubMed] [Europe PMC] [Abstract]
  23. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
  24. "Initial characterization of the human central proteome."BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
  25. "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
  26. "Comparative large-scale characterisation of plant vs. mammal proteins reveals similar and idiosyncratic N-alpha acetylation features."Mol. Cell. Proteomics 11:M111.015131-M111.015131(2012) [PubMed] [Europe PMC] [Abstract]
  27. "N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]
  28. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]
  29. "AUF1 contributes to Cryptochrome1 mRNA degradation and rhythmic translation."Nucleic Acids Res. 42:3590-3606(2014) [PubMed] [Europe PMC] [Abstract]
  30. "Structural roles for human translation factor eIF3 in initiation of protein synthesis."Science 310:1513-1515(2005) [PubMed] [Europe PMC] [Abstract]
  31. "Structure of eIF3b RNA recognition motif and its interaction with eIF3j: structural insights into the recruitment of eIF3b to the 40 S ribosomal subunit."J. Biol. Chem. 282:8165-8174(2007) [PubMed] [Europe PMC] [Abstract]