≡

Eukaryotic Translation Initiation Factor 4E1 (EIF4E1)

[Edit]

CBP; EIF4F; EIF4E; EIF4EL1; eIF-4F 25 kDa subunit; mRNA cap-binding protein

Eukaryotic Translation Initiation Factor 4E1 (EIF4E1)
CCC F013
HGNC 3287
MGI 95305
PubMed 23667251
RGD 69647
UniProt P06730
Wiki EIF4E
All eukaryotic cellular mRNAs are blocked at their 5-prime ends with the 7-methyl-guanosine cap structure, m7GpppX. This structure is involved in several cellular processes including enhanced translational efficiency, splicing, mRNA stability, and RNA nuclear export. EIF4E is a eukaryotic translation initiation factor involved in directing ribosomes to the cap structure of mRNAs. It is a 24-kD polypeptide that exists as both a free form and as part of a multiprotein complex termed EIF4F. The EIF4E polypeptide is the rate-limiting component of the eukaryotic translation apparatus and is involved in the mRNA-ribosome binding step of eukaryotic protein synthesis. The other subunits of EIF4F are a 50-kD polypeptide, termed EIF4A, that possesses ATPase and RNA helicase activities, and a 220-kD polypeptide, EIF4G.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Eukaryotic Translation Initiation Factor 4E1 (EIF4E1) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Eukaryotic Translation Initiation Factor 4E1 (EIF4E1) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Eukaryotic Translation Initiation Factor 4E1 (EIF4E1) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Eukaryotic Translation Initiation Factor 4E1 (EIF4E1) CLIA Kit Customized Service Offer
n/a ELISA Kit for Eukaryotic Translation Initiation Factor 4E1 (EIF4E1) ELISA Kit Customized Service Offer

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Eukaryotic Translation Initiation Factor 4E1 (EIF4E1) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Eukaryotic Translation Initiation Factor 4E1 (EIF4E1) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Eukaryotic Translation Initiation Factor 4E1 (EIF4E1) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Eukaryotic Translation Initiation Factor 4E1 (EIF4E1) CLIA Kit Customized Service Offer
n/a ELISA Kit for Eukaryotic Translation Initiation Factor 4E1 (EIF4E1) ELISA Kit Customized Service Offer

Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Eukaryotic Translation Initiation Factor 4E1 (EIF4E1) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Eukaryotic Translation Initiation Factor 4E1 (EIF4E1) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Eukaryotic Translation Initiation Factor 4E1 (EIF4E1) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Eukaryotic Translation Initiation Factor 4E1 (EIF4E1) CLIA Kit Customized Service Offer
n/a ELISA Kit for Eukaryotic Translation Initiation Factor 4E1 (EIF4E1) ELISA Kit Customized Service Offer
  1. "Amino acid sequence of the mRNA cap-binding protein from human tissues."Proc. Natl. Acad. Sci. U.S.A. 84:945-949(1987) [PubMed] [Europe PMC] [Abstract]
  2. ErratumProc. Natl. Acad. Sci. U.S.A. 89:1148-1148(1992) [PubMed] [Europe PMC] [Abstract]
  3. "Complete sequencing and characterization of 21,243 full-length human cDNAs." Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
  4. "Generation and annotation of the DNA sequences of human chromosomes 2 and 4." Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract]
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
  6. "Transcriptome analysis of human gastric cancer."Mamm. Genome 16:942-954(2005) [PubMed] [Europe PMC] [Abstract]
  7. "Phosphorylation of the proto-oncogene product eukaryotic initiation factor 4E is a common cellular response to tumor necrosis factor."J. Biol. Chem. 266:2685-2688(1991) [PubMed] [Europe PMC] [Abstract]
  8. "Combination of Trp and Glu residues for recognition of mRNA cap structure. Analysis of m7G base recognition site of human cap binding protein (IF-4E) by site-directed mutagenesis."FEBS Lett. 280:207-210(1991) [PubMed] [Europe PMC] [Abstract]
  9. "Phosphorylation site of eukaryotic initiation factor 4E."J. Biol. Chem. 262:10434-10437(1987) [PubMed] [Europe PMC] [Abstract]
  10. "Characterization of wild-type and Ser53 mutant eukaryotic initiation factor 4E overexpression in mammalian cells."J. Biol. Chem. 268:11902-11909(1993) [PubMed] [Europe PMC] [Abstract]
  11. "Role of Ser-53 phosphorylation in the activity of human translation initiation factor eIF-4E in mammalian and yeast cells."Gene 163:283-288(1995) [PubMed] [Europe PMC] [Abstract]
  12. "Phosphorylation of eukaryotic protein synthesis initiation factor 4E at Ser-209."J. Biol. Chem. 270:14597-14603(1995) [PubMed] [Europe PMC] [Abstract]
  13. "Serine 209, not serine 53, is the major site of phosphorylation in initiation factor eIF-4E in serum-treated Chinese hamster ovary cells."J. Biol. Chem. 270:21684-21688(1995) [PubMed] [Europe PMC] [Abstract]
  14. "Repression of cap-dependent translation by 4E-binding protein 1: competition with p220 for binding to eukaryotic initiation factor-4E."EMBO J. 14:5701-5709(1995) [PubMed] [Europe PMC] [Abstract]
  15. "A novel shuttling protein, 4E-T, mediates the nuclear import of the mRNA 5' cap-binding protein, eIF4E."EMBO J. 19:3142-3156(2000) [PubMed] [Europe PMC] [Abstract]
  16. "Human eukaryotic translation initiation factor 4G (eIF4G) recruits mnk1 to phosphorylate eIF4E."EMBO J. 18:270-279(1999) [PubMed] [Europe PMC] [Abstract]
  17. "Eukaryotic initiation factors 4A (eIF4A) and 4G (eIF4G) mutually interact in a 1:1 ratio in vivo."J. Biol. Chem. 276:29111-29115(2001) [PubMed] [Europe PMC] [Abstract]
  18. "The mitogen-activated protein kinase signal-integrating kinase Mnk2 is a eukaryotic initiation factor 4E kinase with high levels of basal activity in mammalian cells."Mol. Cell. Biol. 21:743-754(2001) [PubMed] [Europe PMC] [Abstract]
  19. "The N and C termini of the splice variants of the human mitogen-activated protein kinase-interacting kinase Mnk2 determine activity and localization."Mol. Cell. Biol. 23:5692-5705(2003) [PubMed] [Europe PMC] [Abstract]
  20. "Human retroviral host restriction factors APOBEC3G and APOBEC3F localize to mRNA processing bodies."PLoS Pathog. 2:E41-E41(2006) [PubMed] [Europe PMC] [Abstract]
  21. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
  22. "Deregulation of EIF4E: a novel mechanism for autism."J. Med. Genet. 46:759-765(2009) [PubMed] [Europe PMC] [Abstract]
  23. "The RNA binding protein Larp1 regulates cell division, apoptosis and cell migration."Nucleic Acids Res. 38:5542-5553(2010) [PubMed] [Europe PMC] [Abstract]
  24. "LIM-domain proteins, LIMD1, Ajuba, and WTIP are required for microRNA-mediated gene silencing."Proc. Natl. Acad. Sci. U.S.A. 107:12499-12504(2010) [PubMed] [Europe PMC] [Abstract]
  25. "Initial characterization of the human central proteome."BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
  26. "Translational homeostasis via the mRNA cap-binding protein, eIF4E."Mol. Cell 46:847-858(2012) [PubMed] [Europe PMC] [Abstract]
  27. "Interaction of the eukaryotic initiation factor 4E with 4E-BP2 at a dynamic bipartite interface."Structure 21:2186-2196(2013) [PubMed] [Europe PMC] [Abstract]
  28. "Folding of an intrinsically disordered protein by phosphorylation as a regulatory switch."Nature 519:106-109(2015) [PubMed] [Europe PMC] [Abstract]
  29. "Crystal structures of 7-methylguanosine 5'-triphosphate (m(7)GTP)- and P(1)-7-methylguanosine-P(3)-adenosine-5',5'-triphosphate (m(7)GpppA)-bound human full-length eukaryotic initiation factor 4E: biological importance of the C-terminal flexible region."Biochem. J. 362:539-544(2002) [PubMed] [Europe PMC] [Abstract]
  30. "Backbone resonance assignment of human eukaryotic translation initiation factor 4E (eIF4E) in complex with 7-methylguanosine diphosphate (m7GDP) and a 17-amino acid peptide derived from human eIF4GII."J. Biomol. NMR 27:279-280(2003) [PubMed] [Europe PMC] [Abstract]
  31. "Structural basis for mRNA cap-binding regulation of eukaryotic initiation factor 4E by 4E-binding protein, studied by spectroscopic, X-ray crystal structural, and molecular dynamics simulation methods."Biochim. Biophys. Acta 1753:191-208(2005) [PubMed] [Europe PMC] [Abstract]
  32. "Cap-free structure of eIF4E suggests a basis for conformational regulation by its ligands."EMBO J. 25:5138-5149(2006) [PubMed] [Europe PMC] [Abstract]
  33. "Crystallographic and mass spectrometric characterisation of eIF4E with N7-alkylated cap derivatives."J. Mol. Biol. 372:7-15(2007) [PubMed] [Europe PMC] [Abstract]
  34. "Structural scaffold for eIF4E binding selectivity of 4E-BP isoforms: crystal structure of eIF4E binding region of 4E-BP2 and its comparison with that of 4E-BP1."J. Pept. Sci. 17:650-657(2011) [PubMed] [Europe PMC] [Abstract]