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Mediator Complex Subunit 30 (MED30)

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TRAP25; THRAP6; Thyroid Hormone Receptor Associated Protein 6; Thyroid hormone receptor-associated protein complex 25 kDa component; TRAP/Mediator complex TRAP25

Mediator Complex Subunit 30 (MED30)
CCC F082
HGNC 23032
MGI 1917040
PubMed 22106289
RGD 1309369
UniProt Q96HR3
Wiki MED30
The multiprotein TRAP/Mediator complex facilitates gene expression through a wide variety of transcriptional activators. THRAP6 is a component of this complex that appears to be metazoan specific.
The deduced protein contains 178 amino acids. Northern blot analysis detected ubiquitous expression of a 1-kb TRAP25 mRNA, with elevated levels in heart, placenta, and skeletal muscle.TRAP25 is a component of the TRAP/Mediator complex. Immunodepletion of TRAP25 from nuclear extracts simultaneously depleted other TRAP/Mediator subunits, and depletion of Mediator severely compromised its ability to support high levels of transcriptional activation.The International Radiation Hybrid Mapping Consortium mapped the THRAP6 gene to chromosome 9.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Mediator Complex Subunit 30 (MED30) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Mediator Complex Subunit 30 (MED30) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Mediator Complex Subunit 30 (MED30) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Mediator Complex Subunit 30 (MED30) CLIA Kit Customized Service Offer
n/a ELISA Kit for Mediator Complex Subunit 30 (MED30) ELISA Kit Customized Service Offer

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Mediator Complex Subunit 30 (MED30) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Mediator Complex Subunit 30 (MED30) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Mediator Complex Subunit 30 (MED30) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Mediator Complex Subunit 30 (MED30) CLIA Kit Customized Service Offer
n/a ELISA Kit for Mediator Complex Subunit 30 (MED30) ELISA Kit Customized Service Offer

Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Mediator Complex Subunit 30 (MED30) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Mediator Complex Subunit 30 (MED30) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Mediator Complex Subunit 30 (MED30) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Mediator Complex Subunit 30 (MED30) CLIA Kit Customized Service Offer
n/a ELISA Kit for Mediator Complex Subunit 30 (MED30) ELISA Kit Customized Service Offer
  1. "Requirement of TRAP/mediator for both activator-independent and activator-dependent transcription in conjunction with TFIID-associated TAF(II)s."Mol. Cell. Biol. 22:2842-2852(2002) [PubMed] [Europe PMC] [Abstract]
  2. "DNA sequence and analysis of human chromosome 8." Nature 439:331-335(2006) [PubMed] [Europe PMC] [Abstract]
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
  4. "Identification of mammalian Mediator subunits with similarities to yeast Mediator subunits Srb5, Srb6, Med11, and Rox3."J. Biol. Chem. 278:15123-15127(2003) [PubMed] [Europe PMC] [Abstract]
  5. "A set of consensus mammalian mediator subunits identified by multidimensional protein identification technology."Mol. Cell 14:685-691(2004) [PubMed] [Europe PMC] [Abstract]
  6. "MED1/TRAP220 exists predominantly in a TRAP/Mediator subpopulation enriched in RNA polymerase II and is required for ER-mediated transcription."Mol. Cell 19:89-100(2005) [PubMed] [Europe PMC] [Abstract]
  7. "Human Mediator enhances basal transcription by facilitating recruitment of transcription factor IIB during preinitiation complex assembly."J. Biol. Chem. 281:15172-15181(2006) [PubMed] [Europe PMC] [Abstract]
  8. "Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle."Mol. Cell 31:438-448(2008) [PubMed] [Europe PMC] [Abstract]
  9. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
  10. "Large-scale proteomics analysis of the human kinome."Mol. Cell. Proteomics 8:1751-1764(2009) [PubMed] [Europe PMC] [Abstract]
  11. "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
  12. "N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]