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Mediator Complex Subunit 6 (MED6)

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NY-REN-28; ARC33; Activator-recruited cofactor 33 kDa component; Renal carcinoma antigen NY-REN-28; Mediator of RNA polymerase II transcription subunit 6

Mediator Complex Subunit 6 (MED6)
CCC F096
HGNC 19970
MGI 1917042
PubMed 9234719
RGD 1310296
UniProt O75586
Wiki MED6
One type of intermediary is the mediator complex, which consists of approximately 20 polypeptides. It is physically associated with RNA polymerase II and is essential for transcriptional activation. Lee et al. (1997) found that the S. cerevisiae Med6 protein, a component of the mediator complex, was absolutely required for transcriptional activation of most of the RNA polymerase II-transcribed genes tested; it had no effect on uninduced or repressed transcription, transcription of constitutively expressed genes, or transcription by RNA polymerases I or III. By searching sequence databases with the yeast Med6 protein, Lee et al. (1997) identified an expressed sequence tag containing the complete human MED6 coding sequence. The predicted 157-amino acid human MED6 protein is 30% similar to yeast Med6.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPF096Hu01 Recombinant Mediator Complex Subunit 6 (MED6) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies n/a Monoclonal Antibody to Mediator Complex Subunit 6 (MED6) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Mediator Complex Subunit 6 (MED6) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Mediator Complex Subunit 6 (MED6) CLIA Kit Customized Service Offer
n/a ELISA Kit for Mediator Complex Subunit 6 (MED6) ELISA Kit Customized Service Offer

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Mediator Complex Subunit 6 (MED6) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Mediator Complex Subunit 6 (MED6) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Mediator Complex Subunit 6 (MED6) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Mediator Complex Subunit 6 (MED6) CLIA Kit Customized Service Offer
n/a ELISA Kit for Mediator Complex Subunit 6 (MED6) ELISA Kit Customized Service Offer

Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Mediator Complex Subunit 6 (MED6) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Mediator Complex Subunit 6 (MED6) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Mediator Complex Subunit 6 (MED6) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Mediator Complex Subunit 6 (MED6) CLIA Kit Customized Service Offer
n/a ELISA Kit for Mediator Complex Subunit 6 (MED6) ELISA Kit Customized Service Offer
  1. "NAT, a human complex containing Srb polypeptides that functions as a negative regulator of activated transcription."Mol. Cell 2:213-222(1998) [PubMed] [Europe PMC] [Abstract]
  2. "Complete sequencing and characterization of 21,243 full-length human cDNAs." Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
  3. "The DNA sequence and analysis of human chromosome 14." Nature 421:601-607(2003) [PubMed] [Europe PMC] [Abstract]
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
  5. "Antigens recognized by autologous antibody in patients with renal-cell carcinoma."Int. J. Cancer 83:456-464(1999) [PubMed] [Europe PMC] [Abstract]
  6. "Composite co-activator ARC mediates chromatin-directed transcriptional activation."Nature 398:828-832(1999) [PubMed] [Europe PMC] [Abstract]
  7. "A novel human SRB/MED-containing cofactor complex, SMCC, involved in transcription regulation."Mol. Cell 3:97-108(1999) [PubMed] [Europe PMC] [Abstract]
  8. ErratumMol. Cell 3:541-541(1999)
  9. "TFIIH is negatively regulated by cdk8-containing mediator complexes."Nature 407:102-106(2000) [PubMed] [Europe PMC] [Abstract]
  10. "The TRAP/Mediator coactivator complex interacts directly with estrogen receptors alpha and beta through the TRAP220 subunit and directly enhances estrogen receptor function in vitro."Proc. Natl. Acad. Sci. U.S.A. 99:2642-2647(2002) [PubMed] [Europe PMC] [Abstract]
  11. "A set of consensus mammalian mediator subunits identified by multidimensional protein identification technology."Mol. Cell 14:685-691(2004) [PubMed] [Europe PMC] [Abstract]
  12. "MED1/TRAP220 exists predominantly in a TRAP/Mediator subpopulation enriched in RNA polymerase II and is required for ER-mediated transcription."Mol. Cell 19:89-100(2005) [PubMed] [Europe PMC] [Abstract]
  13. "Regulation of Aurora-A kinase gene expression via GABP recruitment of TRAP220/MED1."J. Biol. Chem. 281:14691-14699(2006) [PubMed] [Europe PMC] [Abstract]
  14. "Human Mediator enhances basal transcription by facilitating recruitment of transcription factor IIB during preinitiation complex assembly."J. Biol. Chem. 281:15172-15181(2006) [PubMed] [Europe PMC] [Abstract]
  15. "Mediator modulates Gli3-dependent Sonic hedgehog signaling."Mol. Cell. Biol. 26:8667-8682(2006) [PubMed] [Europe PMC] [Abstract]
  16. "Lysine acetylation targets protein complexes and co-regulates major cellular functions."Science 325:834-840(2009) [PubMed] [Europe PMC] [Abstract]
  17. "Initial characterization of the human central proteome."BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]