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Splicing Factor 1 (SF1)

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ZFM1; ZNF162; BBP; Zinc Finger Protein 162; mBBP; Mammalian branch point-binding protein; Zinc finger gene in MEN1 locus; Transcription factor ZFM1

Splicing Factor 1 (SF1)
CCC F111
HGNC 12950
MGI 1095403
PubMed 21146534
UniProt Q15637
Wiki SF1
The predicted 623-amino acid protein contains a nuclear transport domain, a metal-binding or zinc finger motif, and glutamine- and proline-rich regions. It shows some sequence similarity to WT1 and EGR2. RT-PCR was used to show expression in the thyroid gland, pancreas, adrenal gland, and ovary.
All of the ZNF162 isoforms contain a KH domain, a sequence motif present in proteins playing a major role in regulating cellular RNA metabolism. The gene contains 14 exons and spans approximately 15 kb. Analysis of the ZNF162 genomic cDNA sequences predicted the use of duplicated 5-prime and 3-prime splice sites as well as exon skipping and intron inclusion to generate 6 ZNF162 mRNAs by alternative splicing events.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPF111Hu01 Recombinant Splicing Factor 1 (SF1) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAF111Hu01 Polyclonal Antibody to Splicing Factor 1 (SF1) WB; IHC; ICC; IP.
Assay Kits n/a CLIA Kit for Splicing Factor 1 (SF1) CLIA Kit Customized Service Offer
n/a ELISA Kit for Splicing Factor 1 (SF1) ELISA Kit Customized Service Offer

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Splicing Factor 1 (SF1) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Splicing Factor 1 (SF1) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Splicing Factor 1 (SF1) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Splicing Factor 1 (SF1) CLIA Kit Customized Service Offer
n/a ELISA Kit for Splicing Factor 1 (SF1) ELISA Kit Customized Service Offer
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  2. "Identification of two novel isoforms of the ZNF162 gene: a growing family of signal transduction and activator of RNA proteins."Genomics 42:268-277(1997) [PubMed] [Europe PMC] [Abstract]
  3. "Isolation and characterization of a novel gene encoding nuclear protein at a locus (D11S636) tightly linked to multiple endocrine neoplasia type 1 (MEN1)."Hum. Mol. Genet. 3:465-470(1994) [PubMed] [Europe PMC] [Abstract]
  4. "Complete sequencing and characterization of 21,243 full-length human cDNAs." Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
  5. "Human chromosome 11 DNA sequence and analysis including novel gene identification." Nature 440:497-500(2006) [PubMed] [Europe PMC] [Abstract]
  6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
  7. "Diverse modes of alternative splicing of human splicing factor SF1 deduced from the exon-intron structure of the gene."Gene 211:29-37(1998) [PubMed] [Europe PMC] [Abstract]
  8. , Submitted (MAY-2006) to UniProtKB
  9. "Phosphorylation of splicing factor SF1 on Ser20 by cGMP-dependent protein kinase regulates spliceosome assembly."EMBO J. 18:4549-4559(1999) [PubMed] [Europe PMC] [Abstract]
  10. "Large-scale proteomic analysis of the human spliceosome."Genome Res. 12:1231-1245(2002) [PubMed] [Europe PMC] [Abstract]
  11. "The transcriptional repressor ZFM1 interacts with and modulates the ability of EWS to activate transcription."J. Biol. Chem. 273:18086-18091(1998) [PubMed] [Europe PMC] [Abstract]
  12. "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract]
  13. "A probability-based approach for high-throughput protein phosphorylation analysis and site localization."Nat. Biotechnol. 24:1285-1292(2006) [PubMed] [Europe PMC] [Abstract]
  14. "U2AF-homology motif interactions are required for alternative splicing regulation by SPF45."Nat. Struct. Mol. Biol. 14:620-629(2007) [PubMed] [Europe PMC] [Abstract]
  15. "Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis."J. Proteome Res. 7:1346-1351(2008) [PubMed] [Europe PMC] [Abstract]
  16. "A quantitative atlas of mitotic phosphorylation."Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
  17. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
  18. "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
  19. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
  20. "Initial characterization of the human central proteome."BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
  21. "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
  22. "Comparative large-scale characterisation of plant vs. mammal proteins reveals similar and idiosyncratic N-alpha acetylation features."Mol. Cell. Proteomics 11:M111.015131-M111.015131(2012) [PubMed] [Europe PMC] [Abstract]
  23. "N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]
  24. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]
  25. "Structural basis for recognition of the intron branch site RNA by splicing factor 1."Science 294:1098-1102(2001) [PubMed] [Europe PMC] [Abstract]
  26. "Structural basis for the molecular recognition between human splicing factors U2AF65 and SF1/mBBP."Mol. Cell 11:965-976(2003) [PubMed] [Europe PMC] [Abstract]