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Ubiquitin Specific Peptidase 5 (USP5)

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ISOT; Isopeptidase T; Ubiquitin Carboxyl-Terminal Hydrolase 5; Ubiquitin Specific Protease 5; Deubiquitinating enzyme 5; Isopeptidase T; Ubiquitin thioesterase 5

Ubiquitin Specific Peptidase 5 (USP5)
CCC F145
HGNC 12628
MGI 1347343
PubMed 19098288
RGD 1308438
UniProt P45974
Wiki USP5
Ubiquitin dependent proteolysis is a complex pathway of protein metabolism implicated in such diverse cellular functions as maintenance of chromatin structure, receptor function, and degradation of abnormal proteins. A late step of the process involves disassembly of the polyubiquitin chains on degraded proteins into ubiquitin monomers.
USP5 disassembles branched polyubiquitin chains by a sequential exo mechanism, starting at the proximal end of the chain . A total of 117 kb of genomic sequence and approximately 11 kb of cDNA sequence was obtained. They identified in this sequence 8 genes, including CD4 and ISOT. Using a battery of strategies, the exon/intron boundaries, splice variants, and tissue expression patterns of the genes were determined.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Ubiquitin Specific Peptidase 5 (USP5) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Ubiquitin Specific Peptidase 5 (USP5) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Ubiquitin Specific Peptidase 5 (USP5) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Ubiquitin Specific Peptidase 5 (USP5) CLIA Kit Customized Service Offer
n/a ELISA Kit for Ubiquitin Specific Peptidase 5 (USP5) ELISA Kit Customized Service Offer

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Ubiquitin Specific Peptidase 5 (USP5) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Ubiquitin Specific Peptidase 5 (USP5) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Ubiquitin Specific Peptidase 5 (USP5) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Ubiquitin Specific Peptidase 5 (USP5) CLIA Kit Customized Service Offer
n/a ELISA Kit for Ubiquitin Specific Peptidase 5 (USP5) ELISA Kit Customized Service Offer

Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Ubiquitin Specific Peptidase 5 (USP5) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Ubiquitin Specific Peptidase 5 (USP5) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Ubiquitin Specific Peptidase 5 (USP5) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Ubiquitin Specific Peptidase 5 (USP5) CLIA Kit Customized Service Offer
n/a ELISA Kit for Ubiquitin Specific Peptidase 5 (USP5) ELISA Kit Customized Service Offer
  1. "cDNA cloning of a human 100 kDa de-ubiquitinating enzyme: the 100 kDa human de-ubiquitinase belongs to the ubiquitin C-terminal hydrolase family 2 (UCH2)."FEBS Lett. 376:233-237(1995) [PubMed] [Europe PMC] [Abstract]
  2. "A gene-rich cluster between the CD4 and triosephosphate isomerase genes at human chromosome 12p13."Genome Res. 6:314-326(1996) [PubMed] [Europe PMC] [Abstract]
  3. "Large-scale sequencing in human chromosome 12p13: experimental and computational gene structure determination."Genome Res. 7:268-280(1997) [PubMed] [Europe PMC] [Abstract]
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
  5. "A human de-ubiquitinating enzyme with both isopeptidase and peptidase activities in vitro."FEBS Lett. 359:73-77(1995) [PubMed] [Europe PMC] [Abstract]
  6. "A probability-based approach for high-throughput protein phosphorylation analysis and site localization."Nat. Biotechnol. 24:1285-1292(2006) [PubMed] [Europe PMC] [Abstract]
  7. "Recognition of polyubiquitin isoforms by the multiple ubiquitin binding modules of isopeptidase T."J. Biol. Chem. 283:19581-19592(2008) [PubMed] [Europe PMC] [Abstract]
  8. "A quantitative atlas of mitotic phosphorylation."Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
  9. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
  10. "Suppression of the deubiquitinating enzyme USP5 causes the accumulation of unanchored polyubiquitin and the activation of p53."J. Biol. Chem. 284:5030-5041(2009) [PubMed] [Europe PMC] [Abstract]
  11. "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
  12. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
  13. "Initial characterization of the human central proteome."BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
  14. "Domain analysis reveals that a deubiquitinating enzyme USP13 performs non-activating catalysis for Lys63-linked polyubiquitin."PLoS ONE 6:E29362-E29362(2011) [PubMed] [Europe PMC] [Abstract]
  15. "Comparative large-scale characterisation of plant vs. mammal proteins reveals similar and idiosyncratic N-alpha acetylation features."Mol. Cell. Proteomics 11:M111.015131-M111.015131(2012) [PubMed] [Europe PMC] [Abstract]
  16. "N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]
  17. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]
  18. "The ubiquitin binding domain ZnF UBP recognizes the C-terminal diglycine motif of unanchored ubiquitin."Cell 124:1197-1208(2006) [PubMed] [Europe PMC] [Abstract]
  19. "Solution structure of the first and second UBA domains in the human ubiquitin specific protease 5 (isopeptidase 5)."Submitted (JUN-2006) to the PDB data bank
  20. "Covalent ubiquitin-USP5 complex."Submitted (DEC-2009) to the PDB data bank