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Cullin 3 (CUL3)

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Cullin 3 (CUL3)
CCC F363
HGNC 2553
MGI 1347360
PubMed 23349464
RGD 1308190
UniProt Q13618
Wiki CUL3
CUL3 is a component of a ubiquitin E3 ligase that is essential for mitotic division. The CUL3/KLHL9/KLHL13 complex was the minimum unit required for correct chromosome alignment in metaphase, proper midzone and midbody formation, and completion of cytokinesis. CUL3/KLHL9/KLHL13 acted as an E3 ligase and regulated dynamic localization of the chromosomal passenger protein Aurora B (AURKB) on mitotic chromosomes and accumulation of Aurora B on the central spindle after anaphase onset. Aurora B directly bound the substrate-recognition domains of KLHL9 and KLHL13 in vitro and coimmunoprecipitated with the CUL3/KLHL9/KLHL13 complex during mitosis. Moreover, Aurora B was ubiquitylated in a CUL3-dependent manner in vivo and by reconstituted CUL3/KLHL9/KLHL13 in vitro.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Cullin 3 (CUL3) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Cullin 3 (CUL3) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Cullin 3 (CUL3) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Cullin 3 (CUL3) CLIA Kit Customized Service Offer
n/a ELISA Kit for Cullin 3 (CUL3) ELISA Kit Customized Service Offer

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Cullin 3 (CUL3) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Cullin 3 (CUL3) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Cullin 3 (CUL3) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Cullin 3 (CUL3) CLIA Kit Customized Service Offer
n/a ELISA Kit for Cullin 3 (CUL3) ELISA Kit Customized Service Offer

Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Cullin 3 (CUL3) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Cullin 3 (CUL3) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Cullin 3 (CUL3) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Cullin 3 (CUL3) CLIA Kit Customized Service Offer
n/a ELISA Kit for Cullin 3 (CUL3) ELISA Kit Customized Service Offer
  1. "Cloning and expression analysis of a novel salicylate suppressible gene, Hs-CUL-3, a member of cullin/Cdc53 family."J. Biol. Chem. 273:24289-24292(1998) [PubMed] [Europe PMC] [Abstract]
  2. "Prediction of the coding sequences of unidentified human genes. X. The complete sequences of 100 new cDNA clones from brain which can code for large proteins in vitro."DNA Res. 5:169-176(1998) [PubMed] [Europe PMC] [Abstract]
  3. "Human CUL-1, but not other cullin family members, selectively interacts with SKP1 to form a complex with SKP2 and cyclin A."Cell Growth Differ. 9:435-449(1998) [PubMed] [Europe PMC] [Abstract]
  4. "Complete sequencing and characterization of 21,243 full-length human cDNAs." Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
  5. "Generation and annotation of the DNA sequences of human chromosomes 2 and 4." Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract]
  6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
  7. "cul-1 is required for cell cycle exit in C. elegans and identifies a novel gene family."Cell 85:829-839(1996) [PubMed] [Europe PMC] [Abstract]
  8. "Cullin-3 targets cyclin E for ubiquitination and controls S phase in mammalian cells."Genes Dev. 13:2375-2387(1999) [PubMed] [Europe PMC] [Abstract]
  9. "In vitro ubiquitination of cyclin D1 by ROC1-CUL1 and ROC1-CUL3."FEBS Lett. 494:181-185(2001) [PubMed] [Europe PMC] [Abstract]
  10. "Covalent modification of all members of human cullin family proteins by NEDD8."Oncogene 18:6829-6834(1999) [PubMed] [Europe PMC] [Abstract]
  11. "ROC1, a homolog of APC11, represents a family of cullin partners with an associated ubiquitin ligase activity."Mol. Cell 3:535-541(1999) [PubMed] [Europe PMC] [Abstract]
  12. "TIP120A associates with cullins and modulates ubiquitin ligase activity."J. Biol. Chem. 278:15905-15910(2003) [PubMed] [Europe PMC] [Abstract]
  13. "Targeting of protein ubiquitination by BTB-Cullin 3-Roc1 ubiquitin ligases."Nat. Cell Biol. 5:1001-1007(2003) [PubMed] [Europe PMC] [Abstract]
  14. "Ubiquitination of Keap1, a BTB-Kelch substrate adaptor protein for Cul3, targets Keap1 for degradation by a proteasome-independent pathway."J. Biol. Chem. 280:30091-30099(2005) [PubMed] [Europe PMC] [Abstract]
  15. "Stable X chromosome inactivation involves the PRC1 Polycomb complex and requires histone MACROH2A1 and the CULLIN3/SPOP ubiquitin E3 ligase."Proc. Natl. Acad. Sci. U.S.A. 102:7635-7640(2005) [PubMed] [Europe PMC] [Abstract]
  16. "BTB domain-containing speckle-type POZ protein (SPOP) serves as an adaptor of Daxx for ubiquitination by Cul3-based ubiquitin ligase."J. Biol. Chem. 281:12664-12672(2006) [PubMed] [Europe PMC] [Abstract]
  17. "A Cul3-based E3 ligase removes Aurora B from mitotic chromosomes, regulating mitotic progression and completion of cytokinesis in human cells."Dev. Cell 12:887-900(2007) [PubMed] [Europe PMC] [Abstract]
  18. "Characterization of cullin-based E3 ubiquitin ligases in intact mammalian cells -- evidence for cullin dimerization."Cell. Signal. 19:1071-1080(2007) [PubMed] [Europe PMC] [Abstract]
  19. "The Cullin3 ubiquitin ligase functions as a Nedd8-bound heterodimer."Mol. Biol. Cell 18:899-909(2007) [PubMed] [Europe PMC] [Abstract]
  20. "KCTD5, a putative substrate adaptor for cullin3 ubiquitin ligases."FEBS J. 275:3900-3910(2008) [PubMed] [Europe PMC] [Abstract]
  21. "Regulation of TIP60 by ATF2 modulates ATM activation."J. Biol. Chem. 283:17605-17614(2008) [PubMed] [Europe PMC] [Abstract]
  22. "The Cul3/Klhdc5 E3 ligase regulates p60/katanin and is required for normal mitosis in mammalian cells."J. Biol. Chem. 284:11663-11675(2009) [PubMed] [Europe PMC] [Abstract]
  23. "The Cul3-KLHL21 E3 ubiquitin ligase targets aurora B to midzone microtubules in anaphase and is required for cytokinesis."J. Cell Biol. 187:791-800(2009) [PubMed] [Europe PMC] [Abstract]
  24. "The Cullin 3 substrate adaptor KLHL20 mediates DAPK ubiquitination to control interferon responses."EMBO J. 29:1748-1761(2010) [PubMed] [Europe PMC] [Abstract]
  25. "Initial characterization of the human central proteome."BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
  26. "Breast cancer metastasis suppressor 1 (BRMS1) is destabilized by the Cul3-SPOP E3 ubiquitin ligase complex."Biochem. Biophys. Res. Commun. 415:720-726(2011) [PubMed] [Europe PMC] [Abstract]
  27. "A Cullin3-KLHL20 Ubiquitin ligase-dependent pathway targets PML to potentiate HIF-1 signaling and prostate cancer progression."Cancer Cell 20:214-228(2011) [PubMed] [Europe PMC] [Abstract]
  28. "PDZ-RhoGEF ubiquitination by Cullin3-KLHL20 controls neurotrophin-induced neurite outgrowth."J. Cell Biol. 193:985-994(2011) [PubMed] [Europe PMC] [Abstract]
  29. "A homozygous mutation in KCTD7 links neuronal ceroid lipofuscinosis to the ubiquitin-proteasome system."Am. J. Hum. Genet. 91:202-208(2012) [PubMed] [Europe PMC] [Abstract]
  30. "Translational homeostasis via the mRNA cap-binding protein, eIF4E."Mol. Cell 46:847-858(2012) [PubMed] [Europe PMC] [Abstract]
  31. "Ubiquitin-dependent regulation of COPII coat size and function."Nature 482:495-500(2012) [PubMed] [Europe PMC] [Abstract]
  32. "N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]
  33. "The CUL3-KLHL3 E3 ligase complex mutated in Gordon's hypertension syndrome interacts with and ubiquitylates WNK isoforms: disease-causing mutations in KLHL3 and WNK4 disrupt interaction."Biochem. J. 451:111-122(2013) [PubMed] [Europe PMC] [Abstract]
  34. "Impaired KLHL3-mediated ubiquitination of WNK4 causes human hypertension."Cell Rep. 3:858-868(2013) [PubMed] [Europe PMC] [Abstract]
  35. "Ubiquitylation-dependent localization of PLK1 in mitosis."Nat. Cell Biol. 15:430-439(2013) [PubMed] [Europe PMC] [Abstract]
  36. "Kelch-like 3 and Cullin 3 regulate electrolyte homeostasis via ubiquitination and degradation of WNK4."Proc. Natl. Acad. Sci. U.S.A. 110:7838-7843(2013) [PubMed] [Europe PMC] [Abstract]
  37. "SCCRO3 (DCUN1D3) antagonizes the neddylation and oncogenic activity of SCCRO (DCUN1D1)."J. Biol. Chem. 289:34728-34742(2014) [PubMed] [Europe PMC] [Abstract]
  38. "K33-linked polyubiquitination of coronin 7 by Cul3-KLHL20 ubiquitin E3 ligase regulates protein trafficking."Mol. Cell 54:586-600(2014) [PubMed] [Europe PMC] [Abstract]
  39. "Adaptor protein self-assembly drives the control of a cullin-RING ubiquitin ligase."Structure 20:1141-1153(2012) [PubMed] [Europe PMC] [Abstract]
  40. "Crystal structure of KLHL3 in complex with Cullin3."PLoS ONE 8:E60445-E60445(2013) [PubMed] [Europe PMC] [Abstract]
  41. "Structural basis for Cul3 assembly with the BTB-Kelch family of E3 ubiquitin ligases."J. Biol. Chem. 288:7803-7814(2013) [PubMed] [Europe PMC] [Abstract]
  42. "Mutations in kelch-like 3 and cullin 3 cause hypertension and electrolyte abnormalities." Nature 482:98-102(2012) [PubMed] [Europe PMC] [Abstract]