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Cullin 4A (CUL4A)

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Cullin 4A (CUL4A)
CCC F364
HGNC 2554
MGI 1914487
PubMed 22422151
RGD 1563853
UniProt Q13619
Wiki CUL4A
CUL4A is the ubiquitin ligase component of a multimeric complex involved in the degradation of DNA damage-response proteins. Human CUL4A and CUL4B are orthologs of nematode Cul4. The partial C-terminal amino acid sequences of CUL4A and CUL4B share 88% identity. Inactivation of CUL4 causes massive DNA rereplication, producing cells with up to 100C DNA content. The C. elegans ortholog of the replication-licensing factor Cdt1 is required for DNA replication. C. elegans CDT1 is present in G1-phase nuclei but disappears as cells enter S phase. In cells lacking CUL4, CDT1 levels failed to decrease during S phase and instead remained constant in the rereplicating cells. Removal of 1 genomic copy of CDT1 suppressed the CUL4 rereplication phenotype.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Cullin 4A (CUL4A) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Cullin 4A (CUL4A) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Cullin 4A (CUL4A) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Cullin 4A (CUL4A) CLIA Kit Customized Service Offer
n/a ELISA Kit for Cullin 4A (CUL4A) ELISA Kit Customized Service Offer

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Cullin 4A (CUL4A) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Cullin 4A (CUL4A) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Cullin 4A (CUL4A) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Cullin 4A (CUL4A) CLIA Kit Customized Service Offer
n/a ELISA Kit for Cullin 4A (CUL4A) ELISA Kit Customized Service Offer

Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Cullin 4A (CUL4A) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Cullin 4A (CUL4A) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Cullin 4A (CUL4A) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Cullin 4A (CUL4A) CLIA Kit Customized Service Offer
n/a ELISA Kit for Cullin 4A (CUL4A) ELISA Kit Customized Service Offer
  1. "The human homologue for the Caenorhabditis elegans cul-4 gene is amplified and overexpressed in primary breast cancers."Cancer Res. 58:3677-3683(1998) [PubMed] [Europe PMC] [Abstract]
  2. "Radiation-mediated proteolysis of CDT1 by CUL4-ROC1 and CSN complexes constitutes a new checkpoint."Nat. Cell Biol. 5:1008-1015(2003) [PubMed] [Europe PMC] [Abstract]
  3. "DDB2, the xeroderma pigmentosum group E gene product, is directly ubiquitylated by Cullin 4A-based ubiquitin ligase complex."DNA Repair 4:537-545(2005) [PubMed] [Europe PMC] [Abstract]
  4. "The DNA sequence and analysis of human chromosome 13." Nature 428:522-528(2004) [PubMed] [Europe PMC] [Abstract]
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
  6. "A new NEDD8-ligating system for cullin-4A."Genes Dev. 12:2263-2268(1998) [PubMed] [Europe PMC] [Abstract]
  7. "cul-1 is required for cell cycle exit in C. elegans and identifies a novel gene family."Cell 85:829-839(1996) [PubMed] [Europe PMC] [Abstract]
  8. "Covalent modification of all members of human cullin family proteins by NEDD8."Oncogene 18:6829-6834(1999) [PubMed] [Europe PMC] [Abstract]
  9. "ROC1, a homolog of APC11, represents a family of cullin partners with an associated ubiquitin ligase activity."Mol. Cell 3:535-541(1999) [PubMed] [Europe PMC] [Abstract]
  10. "The ubiquitin ligase activity in the DDB2 and CSA complexes is differentially regulated by the COP9 signalosome in response to DNA damage."Cell 113:357-367(2003) [PubMed] [Europe PMC] [Abstract]
  11. "CUL-4A stimulates ubiquitylation and degradation of the HOXA9 homeodomain protein."EMBO J. 22:6057-6067(2003) [PubMed] [Europe PMC] [Abstract]
  12. "TIP120A associates with cullins and modulates ubiquitin ligase activity."J. Biol. Chem. 278:15905-15910(2003) [PubMed] [Europe PMC] [Abstract]
  13. "Cul4A physically associates with MDM2 and participates in the proteolysis of p53."Cancer Res. 64:8152-8155(2004) [PubMed] [Europe PMC] [Abstract]
  14. "Targeted ubiquitination of CDT1 by the DDB1-CUL4A-ROC1 ligase in response to DNA damage."Nat. Cell Biol. 6:1003-1009(2004) [PubMed] [Europe PMC] [Abstract]
  15. "Human De-etiolated-1 regulates c-Jun by assembling a CUL4A ubiquitin ligase."Science 303:1371-1374(2004) [PubMed] [Europe PMC] [Abstract]
  16. "Two E3 ubiquitin ligases, SCF-Skp2 and DDB1-Cul4, target human Cdt1 for proteolysis."EMBO J. 25:1126-1136(2006) [PubMed] [Europe PMC] [Abstract]
  17. "DDB1 functions as a linker to recruit receptor WD40 proteins to CUL4-ROC1 ubiquitin ligases."Genes Dev. 20:2949-2954(2006) [PubMed] [Europe PMC] [Abstract]
  18. "Histone H3 and H4 ubiquitylation by the CUL4-DDB-ROC1 ubiquitin ligase facilitates cellular response to DNA damage."Mol. Cell 22:383-394(2006) [PubMed] [Europe PMC] [Abstract]
  19. "A family of diverse Cul4-Ddb1-interacting proteins includes Cdt2, which is required for S phase destruction of the replication factor Cdt1."Mol. Cell 23:709-721(2006) [PubMed] [Europe PMC] [Abstract]
  20. "Cul4A and DDB1 associate with Skp2 to target p27Kip1 for proteolysis involving the COP9 signalosome."Mol. Cell. Biol. 26:2531-2539(2006) [PubMed] [Europe PMC] [Abstract]
  21. "CUL4-DDB1 ubiquitin ligase interacts with multiple WD40-repeat proteins and regulates histone methylation."Nat. Cell Biol. 8:1277-1283(2006) [PubMed] [Europe PMC] [Abstract]
  22. "Characterization of cullin-based E3 ubiquitin ligases in intact mammalian cells -- evidence for cullin dimerization."Cell. Signal. 19:1071-1080(2007) [PubMed] [Europe PMC] [Abstract]
  23. "Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle."Mol. Cell 31:438-448(2008) [PubMed] [Europe PMC] [Abstract]
  24. "A quantitative atlas of mitotic phosphorylation."Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
  25. "A deneddylase encoded by Epstein-Barr virus promotes viral DNA replication by regulating the activity of cullin-RING ligases."Nat. Cell Biol. 12:351-361(2010) [PubMed] [Europe PMC] [Abstract]
  26. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
  27. "Initial characterization of the human central proteome."BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
  28. "Orc2 protects ORCA from ubiquitin-mediated degradation."Cell Cycle 11:3578-3589(2012) [PubMed] [Europe PMC] [Abstract]
  29. "A Cul4 E3 ubiquitin ligase regulates histone hand-off during nucleosome assembly."Cell 155:817-829(2013) [PubMed] [Europe PMC] [Abstract]
  30. "CRL1-FBXO11 promotes Cdt2 ubiquitylation and degradation and regulates Pr-Set7/Set8-mediated cellular migration."Mol. Cell 49:1147-1158(2013) [PubMed] [Europe PMC] [Abstract]
  31. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]
  32. "Molecular architecture and assembly of the DDB1-CUL4A ubiquitin ligase machinery."Nature 443:590-593(2006) [PubMed] [Europe PMC] [Abstract]
  33. "The molecular basis of CRL4DDB2/CSA ubiquitin ligase architecture, targeting, and activation."Cell 147:1024-1039(2011) [PubMed] [Europe PMC] [Abstract]