Midline 2 (MID2)
[Edit]MID1; FXY2; RNF60; TRIM1; Midline-2; RING finger protein 60; Tripartite motif-containing protein 1; Probable E3 ubiquitin-protein ligase MID2; Midline defect 2
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Midline-2 is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to microtubular structures in the cytoplasm. Its function has not been identified. Alternate splicing of this gene results in two transcript variants encoding different isoforms.
MID1 and MID2 display 84% similarity and 77% identity at the protein level, and 70% identity at the nucleotide level. Like MID1, MID2 displays a RING finger, 2 B-boxes, a coiled-coil, and a conserved RFP-like C-terminal domain. The mouse Mid2 protein shows 99.3% identity with human MID2. MID2 appears to be part of a linkage group conserved between mouse and man.
Organism species: Homo sapiens (Human)
| CATALOG NO. | PRODUCT NAME | APPLICATIONS | |
| Proteins | RPF592Hu01 | Recombinant Midline 2 (MID2) | Positive Control; Immunogen; SDS-PAGE; WB. |
| Antibodies | n/a | Monoclonal Antibody to Midline 2 (MID2) | Monoclonal Antibody Customized Service Offer |
| n/a | Polyclonal Antibody to Midline 2 (MID2) | Polyclonal Antibody Customized Service Offer | |
| Assay Kits | n/a | CLIA Kit for Midline 2 (MID2) | CLIA Kit Customized Service Offer |
| n/a | ELISA Kit for Midline 2 (MID2) | ELISA Kit Customized Service Offer |
Organism species: Mus musculus (Mouse)
| CATALOG NO. | PRODUCT NAME | APPLICATIONS | |
| Proteins | n/a | Recombinant Midline 2 (MID2) | Recombinant Protein Customized Service Offer |
| Antibodies | n/a | Monoclonal Antibody to Midline 2 (MID2) | Monoclonal Antibody Customized Service Offer |
| n/a | Polyclonal Antibody to Midline 2 (MID2) | Polyclonal Antibody Customized Service Offer | |
| Assay Kits | n/a | CLIA Kit for Midline 2 (MID2) | CLIA Kit Customized Service Offer |
| n/a | ELISA Kit for Midline 2 (MID2) | ELISA Kit Customized Service Offer |
Organism species: Rattus norvegicus (Rat)
| CATALOG NO. | PRODUCT NAME | APPLICATIONS | |
| Proteins | n/a | Recombinant Midline 2 (MID2) | Recombinant Protein Customized Service Offer |
| Antibodies | n/a | Monoclonal Antibody to Midline 2 (MID2) | Monoclonal Antibody Customized Service Offer |
| n/a | Polyclonal Antibody to Midline 2 (MID2) | Polyclonal Antibody Customized Service Offer | |
| Assay Kits | n/a | CLIA Kit for Midline 2 (MID2) | CLIA Kit Customized Service Offer |
| n/a | ELISA Kit for Midline 2 (MID2) | ELISA Kit Customized Service Offer |
- "FXY2/MID2, a gene related to the X-linked Opitz syndrome gene FXY/MID1, maps to Xq22 and encodes a FNIII domain-containing protein that associates with microtubules."Genomics 62:385-394(1999) [PubMed] [Europe PMC] [Abstract]
- "MID2, a homologue of the Opitz syndrome gene MID1: similarities in a sub-cellular localization and differences in expression during development."Hum. Mol. Genet. 8:1397-1407(1999) [PubMed] [Europe PMC] [Abstract]
- "The DNA sequence of the human X chromosome." Nature 434:325-337(2005) [PubMed] [Europe PMC] [Abstract]
- "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
- "MID1 and MID2 homo- and heterodimerise to tether the rapamycin-sensitive PP2A regulatory subunit, Alpha 4, to microtubules: implications for the clinical variability of X-linked Opitz GBBB syndrome and other developmental disorders."BMC Cell Biol. 3:1-1(2002) [PubMed] [Europe PMC] [Abstract]
- "Targeted deep resequencing identifies MID2 mutation for X-linked intellectual disability with varied disease severity in a large kindred from India."Hum. Mutat. 35:41-44(2014) [PubMed] [Europe PMC] [Abstract]
- "The solution structure of the FN3 domain of human midline 2 protein."Submitted (OCT-2006) to the PDB data bank