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Phosphodiesterase 8A (PDE8A)

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High affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8A

Phosphodiesterase 8A (PDE8A)
CCC F653
HGNC 8793
MGI 1277116
PubMed 20353794
RGD 735125
UniProt O60658
Wiki PDE8A
PDE8A gene contains 23 exons and spans over 80 kb. The 5-prime region of the PDE8A is GC-rich, and it contains a TATA box and numerous transcription factor binding sites, including some involved in cAMP-responsive transcriptional regulation and in expression of genes in T cells.
PDE8A is expressed as an approximately 4.5-kb mRNA in a wide variety of tissues. A recombinant protein containing the 545 C-terminal amino acids of PDE8A displayed very high affinity cAMP-specific PDE activity. Unlike PDE4 and PDE7, the other high affinity cAMP-specific PDEs, PDE8A was not inhibited by IBMX (3-isobutyl-1-methyl-xanthine).

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Phosphodiesterase 8A (PDE8A) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Phosphodiesterase 8A (PDE8A) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Phosphodiesterase 8A (PDE8A) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Phosphodiesterase 8A (PDE8A) CLIA Kit Customized Service Offer
n/a ELISA Kit for Phosphodiesterase 8A (PDE8A) ELISA Kit Customized Service Offer

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Phosphodiesterase 8A (PDE8A) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Phosphodiesterase 8A (PDE8A) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Phosphodiesterase 8A (PDE8A) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Phosphodiesterase 8A (PDE8A) CLIA Kit Customized Service Offer
n/a ELISA Kit for Phosphodiesterase 8A (PDE8A) ELISA Kit Customized Service Offer

Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Phosphodiesterase 8A (PDE8A) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Phosphodiesterase 8A (PDE8A) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Phosphodiesterase 8A (PDE8A) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Phosphodiesterase 8A (PDE8A) CLIA Kit Customized Service Offer
n/a ELISA Kit for Phosphodiesterase 8A (PDE8A) ELISA Kit Customized Service Offer
  1. "Human phosphodiesterase 8A splice variants: cloning, gene organization, and tissue distribution."Gene 280:183-194(2001) [PubMed] [Europe PMC] [Abstract]
  2. "T cell activation up-regulates cyclic nucleotide phosphodiesterases 8A1 and 7A3."Proc. Natl. Acad. Sci. U.S.A. 98:6319-6324(2001) [PubMed] [Europe PMC] [Abstract]
  3. "Complete sequencing and characterization of 21,243 full-length human cDNAs." Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
  4. "Analysis of the DNA sequence and duplication history of human chromosome 15." Nature 440:671-675(2006) [PubMed] [Europe PMC] [Abstract]
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
  6. "Isolation and characterization of PDE8A, a novel human cAMP-specific phosphodiesterase."Biochem. Biophys. Res. Commun. 246:570-577(1998) [PubMed] [Europe PMC] [Abstract]
  7. "Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle."Mol. Cell 31:438-448(2008) [PubMed] [Europe PMC] [Abstract]
  8. "A quantitative atlas of mitotic phosphorylation."Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
  9. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
  10. "Cyclic AMP-specific phosphodiesterase, PDE8A1, is activated by protein kinase A-mediated phosphorylation."FEBS Lett. 586:1631-1637(2012) [PubMed] [Europe PMC] [Abstract]
  11. "Kinetic and structural studies of phosphodiesterase-8A and implication on the inhibitor selectivity."Biochemistry 47:12760-12768(2008) [PubMed] [Europe PMC] [Abstract]