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Polymerase DNA Directed Lambda (POLl)

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BETA-N; DNA polymerase beta-2; DNA polymerase kappa

Polymerase DNA Directed Lambda (POLl)
CCC F727
HGNC 9184
MGI 1889000
PubMed 20348107
RGD 1308053
UniProt Q9UGP5
Wiki POLL
Pol λ is a member of the X family of DNA polymerases. It is thought to resynthesize missing nucleotides during non-homologous end joining, a pathway of DNA double strand break repair. The crystal structure of pol λ shows that, unlike the DNA polymerases that catalyze DNA replication, pol λ makes extensive contacts with the 5' phosphate of the downstream DNA strand. This allows the polymerase to stabilize the two ends of a double-strand break and explains how pol λ is uniquely suited for a role in non-homologous end joining. In addition to NHEJ, pol λ can also participate in base excision repair, where it provides backup activity in the absence of Pol β.Besides its catalytic polymerase domain, pol λ has an 8 kDa domain and a BRCT domain.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Polymerase DNA Directed Lambda (POLl) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Polymerase DNA Directed Lambda (POLl) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Polymerase DNA Directed Lambda (POLl) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Polymerase DNA Directed Lambda (POLl) CLIA Kit Customized Service Offer
n/a ELISA Kit for Polymerase DNA Directed Lambda (POLl) ELISA Kit Customized Service Offer

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Polymerase DNA Directed Lambda (POLl) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Polymerase DNA Directed Lambda (POLl) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Polymerase DNA Directed Lambda (POLl) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Polymerase DNA Directed Lambda (POLl) CLIA Kit Customized Service Offer
n/a ELISA Kit for Polymerase DNA Directed Lambda (POLl) ELISA Kit Customized Service Offer

Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Polymerase DNA Directed Lambda (POLl) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Polymerase DNA Directed Lambda (POLl) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Polymerase DNA Directed Lambda (POLl) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Polymerase DNA Directed Lambda (POLl) CLIA Kit Customized Service Offer
n/a ELISA Kit for Polymerase DNA Directed Lambda (POLl) ELISA Kit Customized Service Offer
  1. "Two novel human and mouse DNA polymerases of the polX family."Nucleic Acids Res. 28:3684-3693(2000) [PubMed] [Europe PMC] [Abstract]
  2. "Identification and characterization of human DNA polymerase beta 2, a DNA polymerase beta-related enzyme."J. Biol. Chem. 275:31233-31238(2000) [PubMed] [Europe PMC] [Abstract]
  3. "The DNA sequence and comparative analysis of human chromosome 10." Nature 429:375-381(2004) [PubMed] [Europe PMC] [Abstract]
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
  5. "Complete sequencing and characterization of 21,243 full-length human cDNAs." Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
  6. "Identification of an intrinsic 5'-deoxyribose-5-phosphate lyase activity in human DNA polymerase lambda: a possible role in base excision repair."J. Biol. Chem. 276:34659-34663(2001) [PubMed] [Europe PMC] [Abstract]
  7. "Human DNA polymerase lambda diverged in evolution from DNA polymerase beta toward specific Mn(++) dependence: a kinetic and thermodynamic study."Biochemistry 42:7467-7476(2003) [PubMed] [Europe PMC] [Abstract]
  8. "Mutagenesis of human DNA polymerase lambda: essential roles of Tyr505 and Phe506 for both DNA polymerase and terminal transferase activities."Nucleic Acids Res. 31:6916-6925(2003) [PubMed] [Europe PMC] [Abstract]
  9. "The human DNA polymerase lambda interacts with PCNA through a domain important for DNA primer binding and the interaction is inhibited by p21/WAF1/CIP1."FASEB J. 18:1743-1745(2004) [PubMed] [Europe PMC] [Abstract]
  10. "DNA elongation by the human DNA polymerase lambda polymerase and terminal transferase activities are differentially coordinated by proliferating cell nuclear antigen and replication protein A."J. Biol. Chem. 280:1971-1981(2005) [PubMed] [Europe PMC] [Abstract]
  11. "Solution structure of the lyase domain of human DNA polymerase lambda."Biochemistry 42:9564-9574(2003) [PubMed] [Europe PMC] [Abstract]
  12. "A structural solution for the DNA polymerase lambda-dependent repair of DNA gaps with minimal homology."Mol. Cell 13:561-572(2004) [PubMed] [Europe PMC] [Abstract]