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Methylthioadenosine Phosphorylase (MTAP)

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MSAP; S-methyl-5'-thioadenosine phosphorylase

Methylthioadenosine Phosphorylase (MTAP)
CCC G011
HGNC 7413
MGI 1914152
PubMed 19567676
UniProt Q13126
Wiki MTAP
Methylthioadenosine phosphorylase (EC 24.2.28) plays a major role in polyamine metabolism and is important for the salvage of both adenine and methionine. For example, as much as 97% of the endogenous adenine produced by human lymphoblasts in culture is formed by catabolism of methylthioadenosine (MeSAdo) by the phosphorylase. MeSAdo, a by-product of the synthesis of the polyamines spermidine and spermine, potently inhibits polyamine aminopropyltransferase reactions if not removed by the above phosphorylase reaction. The encoded enzyme is deficient in many cancers because this gene and the tumor suppressor p16 gene are co-deleted. Multiple alternatively spliced transcript variants have been described for this gene, but their full-length natures remain unknown.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPG011Hu01 Recombinant Methylthioadenosine Phosphorylase (MTAP) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAG011Hu01 Polyclonal Antibody to Methylthioadenosine Phosphorylase (MTAP) WB; IHC; ICC; IP.
MAG011Hu21 Monoclonal Antibody to Methylthioadenosine Phosphorylase (MTAP) WB; IHC; ICC; IP.
Assay Kits SEG011Hu ELISA Kit for Methylthioadenosine Phosphorylase (MTAP) Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPG011Mu01 Recombinant Methylthioadenosine Phosphorylase (MTAP) Positive Control; Immunogen; SDS-PAGE; WB.
RPG011Mu02 Recombinant Methylthioadenosine Phosphorylase (MTAP) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAG011Mu01 Polyclonal Antibody to Methylthioadenosine Phosphorylase (MTAP) WB; IHC; ICC; IP.
PAG011Mu02 Polyclonal Antibody to Methylthioadenosine Phosphorylase (MTAP) WB; IHC; ICC; IP.
Assay Kits SEG011Mu ELISA Kit for Methylthioadenosine Phosphorylase (MTAP) Enzyme-linked immunosorbent assay for Antigen Detection.
  1. "Construction of a 2.8-megabase yeast artificial chromosome contig and cloning of the human methylthioadenosine phosphorylase gene from the tumor suppressor region on 9p21."Proc. Natl. Acad. Sci. U.S.A. 92:6489-6493(1995) [PubMed] [Europe PMC] [Abstract]
  2. "Genomic cloning of methylthioadenosine phosphorylase: a purine metabolic enzyme deficient in multiple different cancers."Proc. Natl. Acad. Sci. U.S.A. 93:6203-6208(1996) [PubMed] [Europe PMC] [Abstract]
  3. "Primate genome gain and loss: a bone dysplasia, muscular dystrophy, and bone cancer syndrome resulting from mutated retroviral-derived MTAP transcripts."Am. J. Hum. Genet. 90:614-627(2012) [PubMed] [Europe PMC] [Abstract]
  4. "DNA sequence and analysis of human chromosome 9." Nature 429:369-374(2004) [PubMed] [Europe PMC] [Abstract]
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
  6. "Purification and characterization of 5'-deoxy-5'-methylthioadenosine phosphorylase from human placenta."J. Biol. Chem. 261:12324-12329(1986) [PubMed] [Europe PMC] [Abstract]
  7. "Purification and characterization of recombinant human 5'-methylthioadenosine phosphorylase: definite identification of coding cDNA."Biochem. Biophys. Res. Commun. 223:514-519(1996) [PubMed] [Europe PMC] [Abstract]
  8. "Methylthioadenosine phosphorylase gene deletions are common in osteosarcoma."Clin. Cancer Res. 8:782-787(2002) [PubMed] [Europe PMC] [Abstract]
  9. "Methylthioadenosine phosphorylase deficiency in Japanese osteosarcoma patients."Int. J. Oncol. 31:1069-1076(2007) [PubMed] [Europe PMC] [Abstract]
  10. "Direct and tumor microenvironment mediated influences of 5'-deoxy-5'-(methylthio)adenosine on tumor progression of malignant melanoma."J. Cell. Biochem. 106:210-219(2009) [PubMed] [Europe PMC] [Abstract]
  11. "Initial characterization of the human central proteome."BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
  12. "Downregulation of methylthioadenosine phosphorylase by homozygous deletion in gastric carcinoma."Genes Chromosomes Cancer 50:421-433(2011) [PubMed] [Europe PMC] [Abstract]
  13. "The structure of human 5'-deoxy-5'-methylthioadenosine phosphorylase at 1.7-A resolution provides insights into substrate binding and catalysis."Structure 7:629-641(1999) [PubMed] [Europe PMC] [Abstract]
  14. "Structural comparison of MTA phosphorylase and MTA/AdoHcy nucleosidase explains substrate preferences and identifies regions exploitable for inhibitor design."Biochemistry 43:5159-5169(2004) [PubMed] [Europe PMC] [Abstract]
  15. "Picomolar transition state analogue inhibitors of human 5'-methylthioadenosine phosphorylase and X-ray structure with MT-immucillin-A."Biochemistry 43:9-18(2004) [PubMed] [Europe PMC] [Abstract]
  16. "The impact of human leukocyte antigen (HLA) micropolymorphism on ligand specificity within the HLA-B*41 allotypic family."Haematologica 96:110-118(2011) [PubMed] [Europe PMC] [Abstract]