≡

Deoxyhypusine Hydroxylase/Monooxygenase (DOHH)

[Edit]

HLRC1; HEAT-Like(PBS Lyase)Repeat Containing 1; Deoxyhypusine dioxygenase; Deoxyhypusine monooxygenase

Deoxyhypusine Hydroxylase/Monooxygenase (DOHH)
CCC G358
HGNC 28662
MGI 1915964
RGD 1304783
UniProt Q9BU89
DOHH encodes a metalloenzyme that catalyzes the last step in the conversion of lysine to the unique amino acid hypusine in eukaryotic initiation factor 5A. The encoded protein hydroxylates deoxyhypusine to form hypusine in the mature eukaryotic initiation factor 5A protein. Alternative splicing results in multiple transcript variants.
DOHH contains 8 heat repeats organized in a symmetrical dyad connected by a variable region. It contains 2 potential metal coordination sites, each composed of 2 strictly conserved his-glu motifs.CD spectroscopy of purified recombinant wildtype DOHH indicated an alpha-helix-rich protein,and site-directed mutagenesis showed that the alpha-helical structure of DOHH does not depend on the conserved his-glu motifs or metal binding.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPG358Hu01 Recombinant Deoxyhypusine Hydroxylase/Monooxygenase (DOHH) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies n/a Monoclonal Antibody to Deoxyhypusine Hydroxylase/Monooxygenase (DOHH) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Deoxyhypusine Hydroxylase/Monooxygenase (DOHH) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Deoxyhypusine Hydroxylase/Monooxygenase (DOHH) CLIA Kit Customized Service Offer
n/a ELISA Kit for Deoxyhypusine Hydroxylase/Monooxygenase (DOHH) ELISA Kit Customized Service Offer

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Deoxyhypusine Hydroxylase/Monooxygenase (DOHH) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Deoxyhypusine Hydroxylase/Monooxygenase (DOHH) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Deoxyhypusine Hydroxylase/Monooxygenase (DOHH) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Deoxyhypusine Hydroxylase/Monooxygenase (DOHH) CLIA Kit Customized Service Offer
n/a ELISA Kit for Deoxyhypusine Hydroxylase/Monooxygenase (DOHH) ELISA Kit Customized Service Offer

Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPG358Ra01 Recombinant Deoxyhypusine Hydroxylase/Monooxygenase (DOHH) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies n/a Monoclonal Antibody to Deoxyhypusine Hydroxylase/Monooxygenase (DOHH) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Deoxyhypusine Hydroxylase/Monooxygenase (DOHH) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Deoxyhypusine Hydroxylase/Monooxygenase (DOHH) CLIA Kit Customized Service Offer
n/a ELISA Kit for Deoxyhypusine Hydroxylase/Monooxygenase (DOHH) ELISA Kit Customized Service Offer
  1. "The DNA sequence and biology of human chromosome 19." Nature 428:529-535(2004) [PubMed] [Europe PMC] [Abstract]
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
  3. "Deoxyhypusine hydroxylase is an Fe(II)-dependent, HEAT-repeat enzyme. Identification of amino acid residues critical for Fe(II) binding and catalysis."J. Biol. Chem. 281:13217-13225(2006) [PubMed] [Europe PMC] [Abstract]
  4. "Molecular cloning, expression, and structural prediction of deoxyhypusine hydroxylase: a HEAT-repeat-containing metalloenzyme."Proc. Natl. Acad. Sci. U.S.A. 103:51-56(2006) [PubMed] [Europe PMC] [Abstract]
  5. "Human deoxyhypusine hydroxylase, an enzyme involved in regulating cell growth, activates O(2) with a nonheme diiron center."Proc. Natl. Acad. Sci. U.S.A. 106:14814-14819(2009) [PubMed] [Europe PMC] [Abstract]
  6. "Initial characterization of the human central proteome."BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
  7. "Comparative large-scale characterisation of plant vs. mammal proteins reveals similar and idiosyncratic N-alpha acetylation features."Mol. Cell. Proteomics 11:M111.015131-M111.015131(2012) [PubMed] [Europe PMC] [Abstract]
  8. "N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]