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Microtubule Associated Protein RP/EB Family, Member 1 (MAPRE1)

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EB1; Adenomatous Polyposis Coli-Binding protein; APC-binding protein EB1; End-binding protein 1

Microtubule Associated Protein RP/EB Family, Member 1 (MAPRE1)
CCC G491
HGNC 6890
MGI 891995
PubMed 22277732
RGD 621781
UniProt Q15691
Wiki MAPRE1
MAPRE1 is a regulator of microtubule dynamics that localizes at both the growing plus ends of microtubules and the centrosome. It is involved in a variety of cellular processes, including establishment and maintenance of cell polarity, search and capture of chromosomes during mitosis, and positioning of the mitotic spindle during asymmetric cell division.
This microtubule localization was abolished in the presence of the microtubule-destabilizing drug nocodazole; upon drug removal, the microtubule distribution of EB1 was recovered and EB1 fluorescence concentrated at the microtubule-organizing center. These results suggested that EB1 is associated with the microtubule network and may be involved in microtubule polymerization and spindle function.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPG491Hu01 Recombinant Microtubule Associated Protein RP/EB Family, Member 1 (MAPRE1) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAG491Hu01 Polyclonal Antibody to Microtubule Associated Protein RP/EB Family, Member 1 (MAPRE1) WB; IHC; ICC/IF
MAG491Hu21 Monoclonal Antibody to Microtubule Associated Protein RP/EB Family, Member 1 (MAPRE1) WB; IHC; ICC; IP.
MAG491Hu22 Monoclonal Antibody to Microtubule Associated Protein RP/EB Family, Member 1 (MAPRE1) WB; IHC; ICC; IP.
MAG491Hu23 Monoclonal Antibody to Microtubule Associated Protein RP/EB Family, Member 1 (MAPRE1) WB; IHC; ICC; IP.
MAG491Hu24 Monoclonal Antibody to Microtubule Associated Protein RP/EB Family, Member 1 (MAPRE1) WB; IHC; ICC; IP.
MAG491Hu25 Monoclonal Antibody to Microtubule Associated Protein RP/EB Family, Member 1 (MAPRE1) WB; IHC; ICC; IP.
MAG491Hu27 Monoclonal Antibody to Microtubule Associated Protein RP/EB Family, Member 1 (MAPRE1) WB; IHC
MAG491Hu29 Monoclonal Antibody to Microtubule Associated Protein RP/EB Family, Member 1 (MAPRE1) WB; IHC; ICC; IP.
Assay Kits SEG491Hu ELISA Kit for Microtubule Associated Protein RP/EB Family, Member 1 (MAPRE1) Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Microtubule Associated Protein RP/EB Family, Member 1 (MAPRE1) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Microtubule Associated Protein RP/EB Family, Member 1 (MAPRE1) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Microtubule Associated Protein RP/EB Family, Member 1 (MAPRE1) Polyclonal Antibody Customized Service Offer
Assay Kits SEG491Mu ELISA Kit for Microtubule Associated Protein RP/EB Family, Member 1 (MAPRE1) Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Microtubule Associated Protein RP/EB Family, Member 1 (MAPRE1) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Microtubule Associated Protein RP/EB Family, Member 1 (MAPRE1) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Microtubule Associated Protein RP/EB Family, Member 1 (MAPRE1) Polyclonal Antibody Customized Service Offer
Assay Kits SEG491Ra ELISA Kit for Microtubule Associated Protein RP/EB Family, Member 1 (MAPRE1) Enzyme-linked immunosorbent assay for Antigen Detection.
  1. "APC binds to the novel protein EB1."Cancer Res. 55:2972-2977(1995) [PubMed] [Europe PMC] [Abstract]
  2. "Complete sequencing and characterization of 21,243 full-length human cDNAs." Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
  3. "The DNA sequence and comparative analysis of human chromosome 20." Nature 414:865-871(2001) [PubMed] [Europe PMC] [Abstract]
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
  5. , Submitted (JUL-2004) to UniProtKB
  6. "The adenomatous polyposis coli-binding protein EB1 is associated with cytoplasmic and spindle microtubules."Proc. Natl. Acad. Sci. U.S.A. 95:10596-10601(1998) [PubMed] [Europe PMC] [Abstract]
  7. "The APC-associated protein EB1 associates with components of the dynactin complex and cytoplasmic dynein intermediate chain."Curr. Biol. 9:425-428(1999) [PubMed] [Europe PMC] [Abstract]
  8. "EB/RP gene family encodes tubulin binding proteins."Int. J. Cancer 81:275-284(1999) [PubMed] [Europe PMC] [Abstract]
  9. "EB3, a novel member of the EB1 family preferentially expressed in the central nervous system, binds to a CNS-specific APC homologue."Oncogene 19:210-216(2000) [PubMed] [Europe PMC] [Abstract]
  10. "Involvement of the telomeric protein Pin2/TRF1 in the regulation of the mitotic spindle."FEBS Lett. 514:193-198(2002) [PubMed] [Europe PMC] [Abstract]
  11. "Evidence that an interaction between EB1 and p150(Glued) is required for the formation and maintenance of a radial microtubule array anchored at the centrosome."Mol. Biol. Cell 13:3627-3645(2002) [PubMed] [Europe PMC] [Abstract]
  12. "Characterization of functional domains of human EB1 family proteins."J. Biol. Chem. 278:49721-49731(2003) [PubMed] [Europe PMC] [Abstract]
  13. "CLASP1 and CLASP2 bind to EB1 and regulate microtubule plus-end dynamics at the cell cortex."J. Cell Biol. 168:141-153(2005) [PubMed] [Europe PMC] [Abstract]
  14. "Immunoaffinity profiling of tyrosine phosphorylation in cancer cells."Nat. Biotechnol. 23:94-101(2005) [PubMed] [Europe PMC] [Abstract]
  15. "Structural basis for tubulin recognition by cytoplasmic linker protein 170 and its autoinhibition."Proc. Natl. Acad. Sci. U.S.A. 104:10346-10351(2007) [PubMed] [Europe PMC] [Abstract]
  16. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
  17. "TIP150 interacts with and targets MCAK at the microtubule plus ends."EMBO Rep. 10:857-865(2009) [PubMed] [Europe PMC] [Abstract]
  18. "Mammalian end binding proteins control persistent microtubule growth."J. Cell Biol. 184:691-706(2009) [PubMed] [Europe PMC] [Abstract]
  19. "Interaction of CDK5RAP2 with EB1 to track growing microtubule tips and to regulate microtubule dynamics."Mol. Biol. Cell 20:3660-3670(2009) [PubMed] [Europe PMC] [Abstract]
  20. "Initial characterization of the human central proteome."BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
  21. "A complex of Kif18b and MCAK promotes microtubule depolymerization and is negatively regulated by Aurora kinases."Curr. Biol. 21:1356-1365(2011) [PubMed] [Europe PMC] [Abstract]
  22. "SLAIN2 links microtubule plus end-tracking proteins and controls microtubule growth in interphase."J. Cell Biol. 193:1083-1099(2011) [PubMed] [Europe PMC] [Abstract]
  23. "Mitotic regulator SKAP forms a link between kinetochore core complex KMN and dynamic spindle microtubules."J. Biol. Chem. 287:39380-39390(2012) [PubMed] [Europe PMC] [Abstract]
  24. "Comparative large-scale characterisation of plant vs. mammal proteins reveals similar and idiosyncratic N-alpha acetylation features."Mol. Cell. Proteomics 11:M111.015131-M111.015131(2012) [PubMed] [Europe PMC] [Abstract]
  25. "N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]
  26. "MISP is a novel Plk1 substrate required for proper spindle orientation and mitotic progression."J. Cell Biol. 200:773-787(2013) [PubMed] [Europe PMC] [Abstract]
  27. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]
  28. "Structural insights into the EB1-APC interaction."EMBO J. 24:261-269(2005) [PubMed] [Europe PMC] [Abstract]
  29. "Crystal structure of the amino-terminal microtubule-binding domain of end-binding protein 1 (EB1)."J. Biol. Chem. 278:36430-36434(2003) [PubMed] [Europe PMC] [Abstract]
  30. "Structural basis for the activation of microtubule assembly by the EB1 and p150Glued complex."Mol. Cell 19:449-460(2005) [PubMed] [Europe PMC] [Abstract]
  31. "Key interaction modes of dynamic +TIP networks."Mol. Cell 23:663-671(2006) [PubMed] [Europe PMC] [Abstract]
  32. "Structural basis of microtubule plus end tracking by XMAP215, CLIP-170, and EB1."Mol. Cell 27:976-991(2007) [PubMed] [Europe PMC] [Abstract]
  33. "An EB1-binding motif acts as a microtubule tip localization signal."Cell 138:366-376(2009) [PubMed] [Europe PMC] [Abstract]