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Phenylethanolamine-N-Methyltransferase (PNMT)

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PENT; Noradrenaline N-methyltransferase

Phenylethanolamine-N-Methyltransferase (PNMT)
CCC G539
HGNC 9160
MGI 97724
PubMed 1426768
RGD 3361
UniProt P11086
Wiki PNMT
Phenylethanolamine N-methyltransferase (PNMT; EC 2.1.1.28) catalyzes the synthesis of epinephrine from norepinephrine, the last step of catecholamine biosynthesis. The nucleotide sequence showed that human PNMT has 282 amino acid residues with a predicted molecular weight of 30,853, including the initial methionine. The amino acid sequence was 88% homologous to that of bovine enzyme.A hybrid gene consisting of 2 kb of the PNMT 5-prime-flanking region fused to the simian virus 40 early region also resulted in tumor antigen mRNA expression in adrenal glands and eyes; furthermore, immunocytochemistry showed that the tumor antigen was localized in nuclei of adrenal medullary cells and cells of the inner nuclear cell layer of the retina, both prominent sites of epinephrine synthesis.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Phenylethanolamine-N-Methyltransferase (PNMT) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Phenylethanolamine-N-Methyltransferase (PNMT) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Phenylethanolamine-N-Methyltransferase (PNMT) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Phenylethanolamine-N-Methyltransferase (PNMT) CLIA Kit Customized Service Offer
n/a ELISA Kit for Phenylethanolamine-N-Methyltransferase (PNMT) ELISA Kit Customized Service Offer

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Phenylethanolamine-N-Methyltransferase (PNMT) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Phenylethanolamine-N-Methyltransferase (PNMT) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Phenylethanolamine-N-Methyltransferase (PNMT) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Phenylethanolamine-N-Methyltransferase (PNMT) CLIA Kit Customized Service Offer
n/a ELISA Kit for Phenylethanolamine-N-Methyltransferase (PNMT) ELISA Kit Customized Service Offer

Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Phenylethanolamine-N-Methyltransferase (PNMT) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Phenylethanolamine-N-Methyltransferase (PNMT) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Phenylethanolamine-N-Methyltransferase (PNMT) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Phenylethanolamine-N-Methyltransferase (PNMT) CLIA Kit Customized Service Offer
n/a ELISA Kit for Phenylethanolamine-N-Methyltransferase (PNMT) ELISA Kit Customized Service Offer
  1. "Molecular cloning of cDNA and chromosomal assignment of the gene for human phenylethanolamine N-methyltransferase, the enzyme for epinephrine biosynthesis."J. Biol. Chem. 263:7672-7677(1988) [PubMed] [Europe PMC] [Abstract]
  2. "Structure of human phenylethanolamine N-methyltransferase gene: existence of two types of mRNA with different transcription initiation sites."Neurochem. Int. 15:555-565(1989)
  3. "Transgenic mice express the human phenylethanolamine N-methyltransferase gene in adrenal medulla and retina."Proc. Natl. Acad. Sci. U.S.A. 85:3648-3652(1988) [PubMed] [Europe PMC] [Abstract]
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
  5. "Getting the adrenaline going: crystal structure of the adrenaline-synthesizing enzyme PNMT."Structure 9:977-985(2001) [PubMed] [Europe PMC] [Abstract]
  6. "Mode of binding of methyl acceptor substrates to the adrenaline-synthesizing enzyme phenylethanolamine N-methyltransferase: implications for catalysis."Biochemistry 44:16875-16885(2005) [PubMed] [Europe PMC] [Abstract]
  7. "Comparison of the binding of 3-fluoromethyl-7-sulfonyl-1,2,3,4-tetrahydroisoquinolines with their isosteric sulfonamides to the active site of phenylethanolamine N-methyltransferase."J. Med. Chem. 49:5424-5433(2006) [PubMed] [Europe PMC] [Abstract]
  8. "Enzyme adaptation to inhibitor binding: a cryptic binding site in phenylethanolamine N-methyltransferase."J. Med. Chem. 50:4845-4853(2007) [PubMed] [Europe PMC] [Abstract]
  9. "Human phenylethanolamine N-methyltransferase pharmacogenomics: gene re-sequencing and functional genomics."J. Neurochem. 95:1766-1776(2005) [PubMed] [Europe PMC] [Abstract]