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Polo Like Kinase 1 (PLK1)

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PLK; STPK13; Serine/threonine-protein kinase 13

Polo Like Kinase 1 (PLK1)
CCC G787
HGNC 9077
MGI 97621
PubMed 8127874
RGD 3352
UniProt P53350
Wiki PLK1
Plk1 consists of 603 amino acids and is 66kDa. In addition to the N-terminus kinase domain, there are two conserved polo-box regions of 30 amino acids at the C-terminus.
Plk1 is an early trigger for G2/M transition. Plk1 supports the functional maturation of the centrosome in late G2/early prophase and establishment of the bipolar spindle. Plk1 phosphorylates and activates cdc25C, a phosphatase that dephosphorylates and activates the cyclinB/cdc2 complex. Plk phosphorylates and activates components of the APC. The APC, which is activated by Fizzy-Cdc20 family proteins, is a cell cycle ubiquitin-protein ligase (E3) that degrades mitotic cyclins, chromosomal proteins that maintain cohesion of sister chromatids, and anaphase inhibitors.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Polo Like Kinase 1 (PLK1) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Polo Like Kinase 1 (PLK1) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Polo Like Kinase 1 (PLK1) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Polo Like Kinase 1 (PLK1) CLIA Kit Customized Service Offer
n/a ELISA Kit for Polo Like Kinase 1 (PLK1) ELISA Kit Customized Service Offer

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Polo Like Kinase 1 (PLK1) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Polo Like Kinase 1 (PLK1) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Polo Like Kinase 1 (PLK1) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Polo Like Kinase 1 (PLK1) CLIA Kit Customized Service Offer
n/a ELISA Kit for Polo Like Kinase 1 (PLK1) ELISA Kit Customized Service Offer

Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Polo Like Kinase 1 (PLK1) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Polo Like Kinase 1 (PLK1) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Polo Like Kinase 1 (PLK1) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Polo Like Kinase 1 (PLK1) CLIA Kit Customized Service Offer
n/a ELISA Kit for Polo Like Kinase 1 (PLK1) ELISA Kit Customized Service Offer
  1. "Cloning and characterization of human and murine homologues of the Drosophila polo serine-threonine kinase."Cell Growth Differ. 5:249-257(1994) [PubMed] [Europe PMC] [Abstract]
  2. "Cell cycle- and terminal differentiation-associated regulation of the mouse mRNA encoding a conserved mitotic protein kinase."Mol. Cell. Biol. 13:7793-7801(1993) [PubMed] [Europe PMC] [Abstract]
  3. "Cell cycle analysis and chromosomal localization of human Plk1, a putative homologue of the mitotic kinases Drosophila polo and Saccharomyces cerevisiae Cdc5."J. Cell Sci. 107:1509-1517(1994) [PubMed] [Europe PMC] [Abstract]
  4. "Induction and down-regulation of PLK, a human serine/threonine kinase expressed in proliferating cells and tumors."Proc. Natl. Acad. Sci. U.S.A. 91:1736-1740(1994) [PubMed] [Europe PMC] [Abstract]
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
  6. "Identification and functional characterization of the human and murine polo-like kinase (Plk) promoter."Oncogene 11:1793-1800(1995) [PubMed] [Europe PMC] [Abstract]
  7. "Cell cycle regulation of the human polo-like kinase (PLK) promoter."J. Biol. Chem. 272:9166-9174(1997) [PubMed] [Europe PMC] [Abstract]
  8. "Antibody microinjection reveals an essential role for human polo-like kinase 1 (Plk1) in the functional maturation of mitotic centrosomes."J. Cell Biol. 135:1701-1713(1996) [PubMed] [Europe PMC] [Abstract]
  9. "The human polo-like kinase, PLK, regulates cdc2/cyclin B through phosphorylation and activation of the cdc25C phosphatase."Cell. Signal. 12:405-411(2000) [PubMed] [Europe PMC] [Abstract]
  10. "Phosphorylation of threonine 210 and the role of serine 137 in the regulation of mammalian polo-like kinase."J. Biol. Chem. 277:44115-44120(2002) [PubMed] [Europe PMC] [Abstract]
  11. "Cooperative phosphorylation including the activity of polo-like kinase 1 regulates the subcellular localization of cyclin B1."Oncogene 21:8282-8292(2002) [PubMed] [Europe PMC] [Abstract]
  12. "Identification of phosphorylation sites in the polo-like kinases Plx1 and Plk1 by a novel strategy based on element and electrospray high resolution mass spectrometry."Proteomics 2:1516-1523(2002) [PubMed] [Europe PMC] [Abstract]
  13. "Polo-like kinase 1 regulates Nlp, a centrosome protein involved in microtubule nucleation."Dev. Cell 5:113-125(2003) [PubMed] [Europe PMC] [Abstract]
  14. "Stk10, a new member of the polo-like kinase kinase family highly expressed in hematopoietic tissue."J. Biol. Chem. 278:18221-18228(2003) [PubMed] [Europe PMC] [Abstract]
  15. "Identification of a consensus motif for Plk (Polo-like kinase) phosphorylation reveals Myt1 as a Plk1 substrate."J. Biol. Chem. 278:25277-25280(2003) [PubMed] [Europe PMC] [Abstract]
  16. "Phosphorylation of mitotic kinesin-like protein 2 by polo-like kinase 1 is required for cytokinesis."J. Cell Biol. 162:863-875(2003) [PubMed] [Europe PMC] [Abstract]
  17. "Active cyclin B1-Cdk1 first appears on centrosomes in prophase."Nat. Cell Biol. 5:143-148(2003) [PubMed] [Europe PMC] [Abstract]
  18. "Ordered proteolysis in anaphase inactivates Plk1 to contribute to proper mitotic exit in human cells."J. Cell Biol. 164:233-241(2004) [PubMed] [Europe PMC] [Abstract]
  19. "Plk1 regulates activation of the anaphase promoting complex by phosphorylating and triggering SCFbetaTrCP-dependent destruction of the APC inhibitor Emi1."Mol. Biol. Cell 15:5623-5634(2004) [PubMed] [Europe PMC] [Abstract]
  20. "M-phase kinases induce phospho-dependent ubiquitination of somatic Wee1 by SCFbeta-TrCP."Proc. Natl. Acad. Sci. U.S.A. 101:4419-4424(2004) [PubMed] [Europe PMC] [Abstract]
  21. "Role of Polo-like kinase in the degradation of early mitotic inhibitor 1, a regulator of the anaphase promoting complex/cyclosome."Proc. Natl. Acad. Sci. U.S.A. 101:7937-7942(2004) [PubMed] [Europe PMC] [Abstract]
  22. "Cdk1/Erk2- and Plk1-dependent phosphorylation of a centrosome protein, Cep55, is required for its recruitment to midbody and cytokinesis."Dev. Cell 9:477-488(2005) [PubMed] [Europe PMC] [Abstract]
  23. "The forkhead-associated domain protein Cep170 interacts with Polo-like kinase 1 and serves as a marker for mature centrioles."Mol. Biol. Cell 16:1095-1107(2005) [PubMed] [Europe PMC] [Abstract]
  24. "The evi5 oncogene regulates cyclin accumulation by stabilizing the anaphase-promoting complex inhibitor emi1."Cell 124:367-380(2006) [PubMed] [Europe PMC] [Abstract]
  25. "Phosphorylation- and polo-box-dependent binding of Plk1 to Bub1 is required for the kinetochore localization of Plk1."Mol. Biol. Cell 17:3705-3716(2006) [PubMed] [Europe PMC] [Abstract]
  26. "The Plk1 target Kizuna stabilizes mitotic centrosomes to ensure spindle bipolarity."Nat. Cell Biol. 8:1095-1101(2006) [PubMed] [Europe PMC] [Abstract]
  27. "Phosphorylation of the cytokinesis regulator ECT2 at G2/M phase stimulates association of the mitotic kinase Plk1 and accumulation of GTP-bound RhoA."Oncogene 25:827-837(2006) [PubMed] [Europe PMC] [Abstract]
  28. "Self-regulated Plk1 recruitment to kinetochores by the Plk1-PBIP1 interaction is critical for proper chromosome segregation."Mol. Cell 24:409-422(2006) [PubMed] [Europe PMC] [Abstract]
  29. "Expression of polo-like kinase 1 (PLK1) protein predicts the survival of patients with gastric carcinoma."Oncology 70:126-133(2006) [PubMed] [Europe PMC] [Abstract]
  30. "A cell proliferation and chromosomal instability signature in anaplastic thyroid carcinoma."Cancer Res. 67:10148-10158(2007) [PubMed] [Europe PMC] [Abstract]
  31. "PICH, a centromere-associated SNF2 family ATPase, is regulated by Plk1 and required for the spindle checkpoint."Cell 128:101-114(2007) [PubMed] [Europe PMC] [Abstract]
  32. "Shugoshin 1 plays a central role in kinetochore assembly and is required for kinetochore targeting of Plk1."Cell Cycle 6:1579-1585(2007) [PubMed] [Europe PMC] [Abstract]
  33. "TTDN1 is a Plk1-interacting protein involved in maintenance of cell cycle integrity."Cell. Mol. Life Sci. 64:632-640(2007) [PubMed] [Europe PMC] [Abstract]
  34. "Polo-like kinase 1 facilitates chromosome alignment during prometaphase through BubR1."J. Biol. Chem. 282:15217-15227(2007) [PubMed] [Europe PMC] [Abstract]
  35. "Expression of PLK1 and survivin in diffuse large B-cell lymphoma."Leuk. Lymphoma 48:2179-2183(2007) [PubMed] [Europe PMC] [Abstract]
  36. "Choice of Plk1 docking partners during mitosis and cytokinesis is controlled by the activation state of Cdk1."Nat. Cell Biol. 9:436-444(2007) [PubMed] [Europe PMC] [Abstract]
  37. "The tumor suppressor CYLD regulates entry into mitosis."Proc. Natl. Acad. Sci. U.S.A. 104:8869-8874(2007) [PubMed] [Europe PMC] [Abstract]
  38. "The Cdc14B-Cdh1-Plk1 axis controls the G2 DNA-damage-response checkpoint."Cell 134:256-267(2008) [PubMed] [Europe PMC] [Abstract]
  39. "Final stages of cytokinesis and midbody ring formation are controlled by BRUCE."Cell 132:832-845(2008) [PubMed] [Europe PMC] [Abstract]
  40. "p73-mediated transcriptional activity is negatively regulated by polo-like kinase 1."Cell Cycle 7:1214-1223(2008) [PubMed] [Europe PMC] [Abstract]
  41. "Plk1 regulates mitotic Aurora A function through betaTrCP-dependent degradation of hBora."Chromosoma 117:457-469(2008) [PubMed] [Europe PMC] [Abstract]
  42. "sSgo1, a major splice variant of Sgo1, functions in centriole cohesion where it is regulated by Plk1."Dev. Cell 14:331-341(2008) [PubMed] [Europe PMC] [Abstract]
  43. "Myosin phosphatase-targeting subunit 1 regulates mitosis by antagonizing polo-like kinase 1."Dev. Cell 14:787-797(2008) [PubMed] [Europe PMC] [Abstract]
  44. "Inhibitory role of Plk1 in the regulation of p73-dependent apoptosis through physical interaction and phosphorylation."J. Biol. Chem. 283:8555-8563(2008) [PubMed] [Europe PMC] [Abstract]
  45. "FAM29A promotes microtubule amplification via recruitment of the NEDD1-gamma-tubulin complex to the mitotic spindle."J. Cell Biol. 183:835-848(2008) [PubMed] [Europe PMC] [Abstract]
  46. "Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle."Mol. Cell 31:438-448(2008) [PubMed] [Europe PMC] [Abstract]
  47. "Plk1-dependent phosphorylation of FoxM1 regulates a transcriptional programme required for mitotic progression."Nat. Cell Biol. 10:1076-1082(2008) [PubMed] [Europe PMC] [Abstract]
  48. "Polo-like kinase-1 is activated by aurora A to promote checkpoint recovery."Nature 455:119-123(2008) [PubMed] [Europe PMC] [Abstract]
  49. "A quantitative atlas of mitotic phosphorylation."Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
  50. "Mammalian BTBD12/SLX4 assembles a Holliday junction resolvase and is required for DNA repair."Cell 138:63-77(2009) [PubMed] [Europe PMC] [Abstract]
  51. "Plk1-mediated phosphorylation of Topors regulates p53 stability."J. Biol. Chem. 284:18588-18592(2009) [PubMed] [Europe PMC] [Abstract]
  52. "Sequential phosphorylation of Nedd1 by Cdk1 and Plk1 is required for targeting of the gammaTuRC to the centrosome."J. Cell Sci. 122:2240-2251(2009) [PubMed] [Europe PMC] [Abstract]
  53. "Plk1 and Aurora A regulate the depolymerase activity and the cellular localization of Kif2a."J. Cell Sci. 122:1334-1341(2009) [PubMed] [Europe PMC] [Abstract]
  54. "Large-scale proteomics analysis of the human kinome."Mol. Cell. Proteomics 8:1751-1764(2009) [PubMed] [Europe PMC] [Abstract]
  55. "Polo-like kinase 1 directs assembly of the HsCyk-4 RhoGAP/Ect2 RhoGEF complex to initiate cleavage furrow formation."PLoS Biol. 7:E1000110-E1000110(2009) [PubMed] [Europe PMC] [Abstract]
  56. "Plk1 self-organization and priming phosphorylation of HsCYK-4 at the spindle midzone regulate the onset of division in human cells."PLoS Biol. 7:E1000111-E1000111(2009) [PubMed] [Europe PMC] [Abstract]
  57. "Multifaceted polo-like kinases: drug targets and antitargets for cancer therapy."Nat. Rev. Drug Discov. 9:643-660(2010) [PubMed] [Europe PMC] [Abstract]
  58. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
  59. "Initial characterization of the human central proteome."BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
  60. "The astrin-kinastrin/SKAP complex localizes to microtubule plus ends and facilitates chromosome alignment."J. Cell Biol. 192:959-968(2011) [PubMed] [Europe PMC] [Abstract]
  61. "Furry protein promotes Aurora A-mediated polo-like kinase 1 activation."J. Biol. Chem. 287:27670-27681(2012) [PubMed] [Europe PMC] [Abstract]
  62. "N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]
  63. "Dynactin helps target Polo-like kinase 1 to kinetochores via its left-handed beta-helical p27 subunit."EMBO J. 32:1023-1035(2013) [PubMed] [Europe PMC] [Abstract]
  64. "MISP is a novel Plk1 substrate required for proper spindle orientation and mitotic progression."J. Cell Biol. 200:773-787(2013) [PubMed] [Europe PMC] [Abstract]
  65. "Ubiquitylation-dependent localization of PLK1 in mitosis."Nat. Cell Biol. 15:430-439(2013) [PubMed] [Europe PMC] [Abstract]
  66. "The molecular basis for phosphodependent substrate targeting and regulation of Plks by the Polo-box domain."Cell 115:83-95(2003) [PubMed] [Europe PMC] [Abstract]
  67. "The crystal structure of the human polo-like kinase-1 polo box domain and its phospho-peptide complex."EMBO J. 22:5757-5768(2003) [PubMed] [Europe PMC] [Abstract]
  68. "Structure of the catalytic domain of human polo-like kinase 1."Biochemistry 46:5960-5971(2007) [PubMed] [Europe PMC] [Abstract]
  69. "Selectivity-determining residues in Plk1."Chem. Biol. Drug Des. 70:540-546(2007) [PubMed] [Europe PMC] [Abstract]
  70. "Molecular and structural basis of polo-like kinase 1 substrate recognition: Implications in centrosomal localization."Proc. Natl. Acad. Sci. U.S.A. 104:3107-3112(2007) [PubMed] [Europe PMC] [Abstract]
  71. "Structure of wild-type Plk-1 kinase domain in complex with a selective DARPin."Acta Crystallogr. D 64:339-353(2008) [PubMed] [Europe PMC] [Abstract]
  72. "Structural and functional analyses of minimal phosphopeptides targeting the polo-box domain of polo-like kinase 1." Nat. Struct. Mol. Biol. 16:876-882(2009) [PubMed] [Europe PMC] [Abstract]
  73. "Patterns of somatic mutation in human cancer genomes." Nature 446:153-158(2007) [PubMed] [Europe PMC] [Abstract]