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Deoxyuridine Triphosphatase (DUT)

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dUTPase; dUTP Pyrophosphatase; Deoxyuridine 5'-triphosphate nucleotidohydrolase, mitochondrial

Deoxyuridine Triphosphatase (DUT)
CCC G875
HGNC 3078
MGI 1346051
PubMed 6130763
RGD 1590931
UniProt P33316
Wiki DUT
DUT encodes an essential enzyme of nucleotide metabolism. The encoded protein forms a ubiquitous, homotetrameric enzyme that hydrolyzes dUTP to dUMP and pyrophosphate. This reaction serves two cellular purposes: providing a precursor (dUMP) for the synthesis of thymine nucleotides needed for DNA replication, and limiting intracellular pools of dUTP. Elevated levels of dUTP lead to increased incorporation of uracil into DNA, which induces extensive excision repair mediated by uracil glycosylase. This repair process, resulting in the removal and reincorporation of dUTP, is self-defeating and leads to DNA fragmentation and cell death. Alternative splicing of this gene leads to different isoforms that localize to either the mitochondrion or nucleus. A related pseudogene is located on chromosome 19.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Deoxyuridine Triphosphatase (DUT) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Deoxyuridine Triphosphatase (DUT) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Deoxyuridine Triphosphatase (DUT) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Deoxyuridine Triphosphatase (DUT) CLIA Kit Customized Service Offer
n/a ELISA Kit for Deoxyuridine Triphosphatase (DUT) ELISA Kit Customized Service Offer

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Deoxyuridine Triphosphatase (DUT) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Deoxyuridine Triphosphatase (DUT) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Deoxyuridine Triphosphatase (DUT) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Deoxyuridine Triphosphatase (DUT) CLIA Kit Customized Service Offer
n/a ELISA Kit for Deoxyuridine Triphosphatase (DUT) ELISA Kit Customized Service Offer

Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Deoxyuridine Triphosphatase (DUT) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Deoxyuridine Triphosphatase (DUT) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Deoxyuridine Triphosphatase (DUT) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Deoxyuridine Triphosphatase (DUT) CLIA Kit Customized Service Offer
n/a ELISA Kit for Deoxyuridine Triphosphatase (DUT) ELISA Kit Customized Service Offer
  1. "Characterization of distinct nuclear and mitochondrial forms of human deoxyuridine triphosphate nucleotidohydrolase."J. Biol. Chem. 271:7745-7751(1996) [PubMed] [Europe PMC] [Abstract]
  2. "Assignment of the human dUTPase gene (DUT) to chromosome 15q15-q21. 1 by fluorescence in situ hybridization."Genomics 40:213-215(1997) [PubMed] [Europe PMC] [Abstract]
  3. "The human dUTPase gene encodes both nuclear and mitochondrial isoforms. Differential expression of the isoforms and characterization of a cDNA encoding the mitochondrial species."J. Biol. Chem. 272:19072-19080(1997) [PubMed] [Europe PMC] [Abstract]
  4. "Complete sequencing and characterization of 21,243 full-length human cDNAs." Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
  6. "Human dUTP pyrophosphatase: cDNA sequence and potential biological importance of the enzyme."Proc. Natl. Acad. Sci. U.S.A. 89:8020-8024(1992) [PubMed] [Europe PMC] [Abstract]
  7. ErratumProc. Natl. Acad. Sci. U.S.A. 90:4328-4328(1993)
  8. "Maturation stage and proliferation-dependent expression of dUTPase in human T cells."Proc. Natl. Acad. Sci. U.S.A. 90:4991-4995(1993) [PubMed] [Europe PMC] [Abstract]
  9. "Identification of a consensus cyclin-dependent kinase phosphorylation site unique to the nuclear form of human deoxyuridine triphosphate nucleotidohydrolase."J. Biol. Chem. 271:7752-7757(1996) [PubMed] [Europe PMC] [Abstract]
  10. "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract]
  11. "A probability-based approach for high-throughput protein phosphorylation analysis and site localization."Nat. Biotechnol. 24:1285-1292(2006) [PubMed] [Europe PMC] [Abstract]
  12. "A quantitative atlas of mitotic phosphorylation."Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
  13. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
  14. "Initial characterization of the human central proteome."BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
  15. "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
  16. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]
  17. "Human dUTP pyrophosphatase: uracil recognition by a beta hairpin and active sites formed by three separate subunits."Structure 4:1077-1092(1996) [PubMed] [Europe PMC] [Abstract]
  18. "Active site closure facilitates juxtaposition of reactant atoms for initiation of catalysis by human dUTPase."FEBS Lett. 581:4783-4788(2007) [PubMed] [Europe PMC] [Abstract]