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Thymidylate Synthetase (TYMS)

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TS; TMS; TSase; Thymidylate Synthase

Thymidylate Synthetase (TYMS)
CCC G963
HGNC 12441
MGI 98878
PubMed 3099389
RGD 3921
UniProt P04818
Wiki TYMS
Thymidylate synthetase (EC 2.1.1.45) is the enzyme used to generate thymidine monophosphate (dTMP), which is subsequently phosphorylated to thymidine triphosphate for use in DNA synthesis and repair. By means of reductive methylation, deoxyuridine monophosphate (dUMP) and N5,N10-methylene tetrahydrofolate are together used to form dTMP, yielding dihydrofolate as a secondary product.
As an anti-cancer chemotherapy target, thymidylate synthetase can be inhibited by the thymidylate synthase inhibitors such as fluorinated pyrimidine fluorouracil, or certain folate analogues, including most notably raltitrexed (trade name Tomudex).

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPG963Hu01 Recombinant Thymidylate Synthetase (TYMS) Positive Control; Immunogen; SDS-PAGE; WB.
APG963Hu01 Active Thymidylate Synthetase (TYMS) Cell culture; Activity Assays.
Antibodies PAG963Hu01 Polyclonal Antibody to Thymidylate Synthetase (TYMS) WB,IHC
MAG963Hu21 Monoclonal Antibody to Thymidylate Synthetase (TYMS) WB; IHC; ICC; IP.
Assay Kits SEG963Hu ELISA Kit for Thymidylate Synthetase (TYMS) Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Thymidylate Synthetase (TYMS) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Thymidylate Synthetase (TYMS) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Thymidylate Synthetase (TYMS) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Thymidylate Synthetase (TYMS) CLIA Kit Customized Service Offer
n/a ELISA Kit for Thymidylate Synthetase (TYMS) ELISA Kit Customized Service Offer

Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Thymidylate Synthetase (TYMS) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Thymidylate Synthetase (TYMS) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Thymidylate Synthetase (TYMS) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Thymidylate Synthetase (TYMS) CLIA Kit Customized Service Offer
n/a ELISA Kit for Thymidylate Synthetase (TYMS) ELISA Kit Customized Service Offer
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  2. "Structural and functional analysis of the human thymidylate synthase gene."J. Biol. Chem. 265:20277-20284(1990) [PubMed] [Europe PMC] [Abstract]
  3. "Differential alternative splicing expressions of thymidylate synthase isoforms."Cancer Lett. 193:127-131(2003) [PubMed] [Europe PMC] [Abstract]
  4. "DNA sequence and analysis of human chromosome 18." Nature 437:551-555(2005) [PubMed] [Europe PMC] [Abstract]
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
  6. "Human thymidylate synthase gene: isolation of phage clones which cover a functionally active gene and structural analysis of the region upstream from the translation initiation codon."J. Biochem. 106:575-583(1989) [PubMed] [Europe PMC] [Abstract]
  7. "Purification and NH2-terminal amino acid sequence of human thymidylate synthase in an overproducing transformant of mouse FM3A cells."J. Biochem. 97:845-850(1985) [PubMed] [Europe PMC] [Abstract]
  8. "Expression of human thymidylate synthase in Escherichia coli."J. Biol. Chem. 264:9145-9148(1989) [PubMed] [Europe PMC] [Abstract]
  9. "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
  10. "Initial characterization of the human central proteome."BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
  11. "Identification of a de novo thymidylate biosynthesis pathway in mammalian mitochondria."Proc. Natl. Acad. Sci. U.S.A. 108:15163-15168(2011) [PubMed] [Europe PMC] [Abstract]
  12. "N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]
  13. "Crystal structure of human thymidylate synthase: a structural mechanism for guiding substrates into the active site."Biochemistry 34:16279-16287(1995) [PubMed] [Europe PMC] [Abstract]
  14. "Human thymidylate synthase is in the closed conformation when complexed with dUMP and raltitrexed, an antifolate drug."Biochemistry 40:1897-1902(2001) [PubMed] [Europe PMC] [Abstract]
  15. "Structure of human thymidylate synthase suggests advantages of chemotherapy with noncompetitive inhibitors."J. Biol. Chem. 276:14170-14177(2001) [PubMed] [Europe PMC] [Abstract]