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SRSF Protein Kinase 1 (SRPK1)

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SFRSK1; SFRS Protein Kinase 1; Serine/arginine-rich protein-specific kinase 1

SRSF Protein Kinase 1 (SRPK1)
CCC H131
HGNC 11305
MGI 106908
PubMed 22172722
RGD 1308272
UniProt Q96SB4
Wiki SRPK1
SRPK1 localizes to the nucleus and the cytoplasm. It is thought to play a role in regulation of both constitutive and alternative splicing by regulating intracellular localization of splicing factors. A second alternatively spliced transcript variant for this gene has been described, but its full length nature has not been determined.The SRPK1 gene was cloned; it encodes a protein of 655 amino acids. SRPK1 is similar to a hypothetical C. elegans kinase, CEHK, and to the fission yeast kinase Dsk1. The association of phosphorylation of SR proteins in vivo with SRPK1 activity in the extracts, together with the fact that SRPK1 was the only major kinase for SR proteins obtained during purification, strongly suggests that SRPK1 is responsible for phosphorylation of SR proteins during the cell cycle in vivo.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant SRSF Protein Kinase 1 (SRPK1) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to SRSF Protein Kinase 1 (SRPK1) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to SRSF Protein Kinase 1 (SRPK1) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for SRSF Protein Kinase 1 (SRPK1) CLIA Kit Customized Service Offer
n/a ELISA Kit for SRSF Protein Kinase 1 (SRPK1) ELISA Kit Customized Service Offer

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant SRSF Protein Kinase 1 (SRPK1) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to SRSF Protein Kinase 1 (SRPK1) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to SRSF Protein Kinase 1 (SRPK1) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for SRSF Protein Kinase 1 (SRPK1) CLIA Kit Customized Service Offer
n/a ELISA Kit for SRSF Protein Kinase 1 (SRPK1) ELISA Kit Customized Service Offer

Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant SRSF Protein Kinase 1 (SRPK1) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to SRSF Protein Kinase 1 (SRPK1) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to SRSF Protein Kinase 1 (SRPK1) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for SRSF Protein Kinase 1 (SRPK1) CLIA Kit Customized Service Offer
n/a ELISA Kit for SRSF Protein Kinase 1 (SRPK1) ELISA Kit Customized Service Offer
  1. "A serine kinase regulates intracellular localization of splicing factors in the cell cycle."Nature 369:678-682(1994) [PubMed] [Europe PMC] [Abstract]
  2. "Complete sequencing and characterization of 21,243 full-length human cDNAs." Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
  3. "Cloning and characterization of an alternatively spliced form of SR protein kinase 1 that interacts specifically with scaffold attachment factor-B."J. Biol. Chem. 276:40175-40182(2001) [PubMed] [Europe PMC] [Abstract].
  4. "Identification of SRPK1 and SRPK2 as the major cellular protein kinases phosphorylating hepatitis B virus core protein."J. Virol. 76:8124-8137(2002) [PubMed] [Europe PMC] [Abstract]
  5. "Identification of toposome, a novel multisubunit complex containing topoisomerase IIalpha."Cell Cycle 3:638-647(2004) [PubMed] [Europe PMC] [Abstract]
  6. "A protein related to splicing factor U2AF35 that interacts with U2AF65 and SR proteins in splicing of pre-mRNA."Nature 388:397-400(1997) [PubMed] [Europe PMC] [Abstract]
  7. "SRPK1 and LBR protein kinases show identical substrate specificities."Biochem. Biophys. Res. Commun. 255:602-607(1999) [PubMed] [Europe PMC] [Abstract]
  8. "SR protein-specific kinase 1 is highly expressed in testis and phosphorylates protamine 1."Nucleic Acids Res. 27:2972-2980(1999) [PubMed] [Europe PMC] [Abstract]
  9. "Processive phosphorylation of alternative splicing factor/splicing factor 2."Proc. Natl. Acad. Sci. U.S.A. 100:12601-12606(2003) [PubMed] [Europe PMC] [Abstract]
  10. "Protein kinase CK2 phosphorylates and activates the SR protein-specific kinase 1."Biochem. Biophys. Res. Commun. 301:650-656(2003) [PubMed] [Europe PMC] [Abstract]
  11. "Suppression of hepatitis B virus replication by SRPK1 and SRPK2 via a pathway independent of the phosphorylation of the viral core protein."Virology 342:150-158(2005) [PubMed] [Europe PMC] [Abstract]
  12. "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract]
  13. "Ordered multi-site phosphorylation of the splicing factor ASF/SF2 by SRPK1."J. Mol. Biol. 376:55-68(2008) [PubMed] [Europe PMC] [Abstract]
  14. "Adaptable molecular interactions guide phosphorylation of the SR protein ASF/SF2 by SRPK1."J. Mol. Biol. 382:894-909(2008) [PubMed] [Europe PMC] [Abstract]
  15. "Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle."Mol. Cell 31:438-448(2008) [PubMed] [Europe PMC] [Abstract]
  16. "A quantitative atlas of mitotic phosphorylation."Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
  17. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
  18. "Allosteric interactions direct binding and phosphorylation of ASF/SF2 by SRPK1."Biochemistry 48:11432-11440(2009) [PubMed] [Europe PMC] [Abstract]
  19. "The enzymatic activity of SR protein kinases 1 and 1a is negatively affected by interaction with scaffold attachment factors B1 and 2."FEBS J. 276:5212-5227(2009) [PubMed] [Europe PMC] [Abstract]
  20. "Regulation of SR protein phosphorylation and alternative splicing by modulating kinetic interactions of SRPK1 with molecular chaperones."Genes Dev. 23:482-495(2009) [PubMed] [Europe PMC] [Abstract]
  21. "Regiospecific phosphorylation control of the SR protein ASF/SF2 by SRPK1."J. Mol. Biol. 390:618-634(2009) [PubMed] [Europe PMC] [Abstract]
  22. "Arginine methylation of the ICP27 RGG box regulates the functional interactions of ICP27 with SRPK1 and Aly/REF during herpes simplex virus 1 infection."J. Virol. 83:8970-8975(2009) [PubMed] [Europe PMC] [Abstract]
  23. "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
  24. "The ratio of SRPK1/SRPK1a regulates erythroid differentiation in K562 leukaemic cells."Biochim. Biophys. Acta 1803:1319-1331(2010) [PubMed] [Europe PMC] [Abstract]
  25. "Initial characterization of the human central proteome."BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
  26. "Acetylation and phosphorylation of SRSF2 control cell fate decision in response to cisplatin."EMBO J. 30:510-523(2011) [PubMed] [Europe PMC] [Abstract]
  27. "Serine-arginine protein kinases: a small protein kinase family with a large cellular presence."FEBS J. 278:570-586(2011) [PubMed] [Europe PMC] [Abstract]
  28. "Phosphorylation mechanism and structure of serine-arginine protein kinases."FEBS J. 278:587-597(2011) [PubMed] [Europe PMC] [Abstract]
  29. "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
  30. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]
  31. "Interplay between SRPK and Clk/Sty kinases in phosphorylation of the splicing factor ASF/SF2 is regulated by a docking motif in ASF/SF2."Mol. Cell 20:77-89(2005) [PubMed] [Europe PMC] [Abstract]
  32. "SR protein kinase 1 is resilient to inactivation."Structure 15:123-133(2007) [PubMed] [Europe PMC] [Abstract]
  33. "A sliding docking interaction is essential for sequential and processive phosphorylation of an SR protein by SRPK1."Mol. Cell 29:563-576(2008) [PubMed] [Europe PMC] [Abstract]