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Replication Protein A3 (RPA3)

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REPA3; RP-A p14; Replication protein A 14 kDa subunit

Replication Protein A3 (RPA3)
CCC H215
HGNC 10291
MGI 1915490
PubMed 20885790
RGD 1306810
UniProt P35244
Wiki RPA3
The RPA3 gene has a 692-basepair sequence with an open reading frame encoding a protein of 121 amino acids. The deduced amino acid sequence showed only limited similarity to the small subunit of yeast RPA. See also RPA1 and RPA2.
In vitro, RPA stimulates the binding of ATRIP to single-stranded DNA. The binding of ATRIP to RPA-coated single-stranded DNA enables the ATR-ATRIP complex to associate with DNA and stimulates phosphorylation of the RAD17 protein that is bound to DNA. Furthermore, Ddc2, the budding yeast homolog of ATRIP, is specifically recruited to double-stranded DNA breaks in an RPA-dependent manner. A checkpoint-deficient mutant of RPA, rfa1-t11, is defective for recruiting Ddc2 to single-stranded DNA both in vivo and in vitro.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Replication Protein A3 (RPA3) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Replication Protein A3 (RPA3) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Replication Protein A3 (RPA3) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Replication Protein A3 (RPA3) CLIA Kit Customized Service Offer
n/a ELISA Kit for Replication Protein A3 (RPA3) ELISA Kit Customized Service Offer

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Replication Protein A3 (RPA3) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Replication Protein A3 (RPA3) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Replication Protein A3 (RPA3) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Replication Protein A3 (RPA3) CLIA Kit Customized Service Offer
n/a ELISA Kit for Replication Protein A3 (RPA3) ELISA Kit Customized Service Offer

Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Replication Protein A3 (RPA3) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Replication Protein A3 (RPA3) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Replication Protein A3 (RPA3) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Replication Protein A3 (RPA3) CLIA Kit Customized Service Offer
n/a ELISA Kit for Replication Protein A3 (RPA3) ELISA Kit Customized Service Offer
  1. "Cloning, overexpression, and genomic mapping of the 14-kDa subunit of human replication protein A."J. Biol. Chem. 268:6131-6138(1993) [PubMed] [Europe PMC] [Abstract]
  2. "The DNA sequence of human chromosome 7." Nature 424:157-164(2003) [PubMed] [Europe PMC] [Abstract]
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
  4. "Mammalian DNA nucleotide excision repair reconstituted with purified protein components."Cell 80:859-868(1995) [PubMed] [Europe PMC] [Abstract]
  5. "Rpa4, a homolog of the 34-kilodalton subunit of the replication protein A complex."Mol. Cell. Biol. 15:3119-3128(1995) [PubMed] [Europe PMC] [Abstract]
  6. "The evolutionarily conserved zinc finger motif in the largest subunit of human replication protein A is required for DNA replication and mismatch repair but not for nucleotide excision repair."J. Biol. Chem. 273:1453-1461(1998) [PubMed] [Europe PMC] [Abstract]
  7. "Replication protein A stimulates long patch DNA base excision repair."J. Biol. Chem. 273:27492-27498(1998) [PubMed] [Europe PMC] [Abstract]
  8. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
  9. "An alternative form of replication protein a prevents viral replication in vitro."J. Biol. Chem. 284:5324-5331(2009) [PubMed] [Europe PMC] [Abstract]
  10. "Evidence for direct contact between the RPA3 subunit of the human replication protein A and single-stranded DNA."Nucleic Acids Res. 37:38-46(2009) [PubMed] [Europe PMC] [Abstract]
  11. "An alternative form of replication protein a expressed in normal human tissues supports DNA repair."J. Biol. Chem. 285:4788-4797(2010) [PubMed] [Europe PMC] [Abstract]
  12. "Initial characterization of the human central proteome."BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
  13. "Comparative large-scale characterisation of plant vs. mammal proteins reveals similar and idiosyncratic N-alpha acetylation features."Mol. Cell. Proteomics 11:M111.015131-M111.015131(2012) [PubMed] [Europe PMC] [Abstract]
  14. "N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]
  15. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]
  16. "PRP19 transforms into a sensor of RPA-ssDNA after DNA damage and drives ATR activation via a ubiquitin-mediated circuitry."Mol. Cell 53:235-246(2014) [PubMed] [Europe PMC] [Abstract]
  17. "Structure of the RPA trimerization core and its role in the multistep DNA-binding mechanism of RPA."EMBO J. 21:1855-1863(2002) [PubMed] [Europe PMC] [Abstract]