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Replication Protein A2 (RPA2)

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REPA2; RPA32; RP-A p34; Replication protein A 32 kDa subunit; Replication protein A 34 kDa subunit

Replication Protein A2 (RPA2)
CCC H216
HGNC 10290
MGI 1339939
PubMed 20130019
RGD 619714
UniProt P15927
Wiki RPA2
Replication protein A is a 3-subunit single-stranded DNA (ssDNA)-binding protein that has been isolated from human cells and found to be essential for in vitro replication of the papovavirus SV40.The open reading frame predicted a protein of 270 amino acids, including the N-terminal methionine residue. The N-terminal region was particularly rich in glycine and serine.The binding of ATRIP to RPA-coated single-stranded DNA enables the ATR-ATRIP complex to associate with DNA and stimulates phosphorylation of the RAD17 protein that is bound to DNA. Furthermore, Ddc2, the budding yeast homolog of ATRIP, is specifically recruited to double-stranded DNA breaks in an RPA-dependent manner. A checkpoint-deficient mutant of RPA, rfa1-t11, is defective for recruiting Ddc2 to single-stranded DNA both in vivo and in vitro.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Replication Protein A2 (RPA2) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Replication Protein A2 (RPA2) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Replication Protein A2 (RPA2) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Replication Protein A2 (RPA2) CLIA Kit Customized Service Offer
n/a ELISA Kit for Replication Protein A2 (RPA2) ELISA Kit Customized Service Offer

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Replication Protein A2 (RPA2) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Replication Protein A2 (RPA2) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Replication Protein A2 (RPA2) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Replication Protein A2 (RPA2) CLIA Kit Customized Service Offer
n/a ELISA Kit for Replication Protein A2 (RPA2) ELISA Kit Customized Service Offer

Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Replication Protein A2 (RPA2) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Replication Protein A2 (RPA2) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Replication Protein A2 (RPA2) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Replication Protein A2 (RPA2) CLIA Kit Customized Service Offer
n/a ELISA Kit for Replication Protein A2 (RPA2) ELISA Kit Customized Service Offer
  1. "The primary structure of the 32-kDa subunit of human replication protein A."J. Biol. Chem. 265:3177-3182(1990) [PubMed] [Europe PMC] [Abstract]
  2. "The DNA sequence and biological annotation of human chromosome 1." Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract]
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
  4. "Cell-cycle-regulated phosphorylation of DNA replication factor A from human and yeast cells."Genes Dev. 4:968-977(1990) [PubMed] [Europe PMC] [Abstract]
  5. "cdc2 family kinases phosphorylate a human cell DNA replication factor, RPA, and activate DNA replication."EMBO J. 11:2189-2199(1992) [PubMed] [Europe PMC] [Abstract]
  6. "The ionizing radiation-induced replication protein A phosphorylation response differs between ataxia telangiectasia and normal human cells."Mol. Cell. Biol. 13:7222-7231(1993) [PubMed] [Europe PMC] [Abstract]
  7. "Mammalian DNA nucleotide excision repair reconstituted with purified protein components."Cell 80:859-868(1995) [PubMed] [Europe PMC] [Abstract]
  8. "RPA involvement in the damage-recognition and incision steps of nucleotide excision repair."Nature 374:566-569(1995) [PubMed] [Europe PMC] [Abstract]
  9. "Physical interaction between human RAD52 and RPA is required for homologous recombination in mammalian cells."J. Biol. Chem. 271:18996-19000(1996) [PubMed] [Europe PMC] [Abstract]
  10. "Mapping of amino acid residues in the p34 subunit of human single-stranded DNA-binding protein phosphorylated by DNA-dependent protein kinase and Cdc2 kinase in vitro."J. Biol. Chem. 272:12634-12641(1997) [PubMed] [Europe PMC] [Abstract]
  11. "Sites of UV-induced phosphorylation of the p34 subunit of replication protein A from HeLa cells."J. Biol. Chem. 272:23896-23904(1997) [PubMed] [Europe PMC] [Abstract]
  12. "The evolutionarily conserved zinc finger motif in the largest subunit of human replication protein A is required for DNA replication and mismatch repair but not for nucleotide excision repair."J. Biol. Chem. 273:1453-1461(1998) [PubMed] [Europe PMC] [Abstract]
  13. "Replication protein A stimulates long patch DNA base excision repair."J. Biol. Chem. 273:27492-27498(1998) [PubMed] [Europe PMC] [Abstract]
  14. "RBT1, a novel transcriptional co-activator, binds the second subunit of replication protein A."Nucleic Acids Res. 28:3478-3485(2000) [PubMed] [Europe PMC] [Abstract]
  15. "ATR kinase activity regulates the intranuclear translocation of ATR and RPA following ionizing radiation."Curr. Biol. 13:1047-1051(2003) [PubMed] [Europe PMC] [Abstract]
  16. "Coordinated regulation of replication protein A activities by its subunits p14 and p32."J. Biol. Chem. 279:35368-35376(2004) [PubMed] [Europe PMC] [Abstract]
  17. "Mammalian TIMELESS and Tipin are evolutionarily conserved replication fork-associated factors."J. Mol. Biol. 366:36-52(2007) [PubMed] [Europe PMC] [Abstract]
  18. "RPA mediates recombination repair during replication stress and is displaced from DNA by checkpoint signalling in human cells."J. Mol. Biol. 373:38-47(2007) [PubMed] [Europe PMC] [Abstract]
  19. "The human Tim/Tipin complex coordinates an Intra-S checkpoint response to UV that slows replication fork displacement."Mol. Cell. Biol. 27:3131-3142(2007) [PubMed] [Europe PMC] [Abstract]
  20. "Replication protein A prevents accumulation of single-stranded telomeric DNA in cells that use alternative lengthening of telomeres."Nucleic Acids Res. 35:7267-7278(2007) [PubMed] [Europe PMC] [Abstract]
  21. "A quantitative atlas of mitotic phosphorylation."Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
  22. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
  23. "The annealing helicase HARP is recruited to DNA repair sites via an interaction with RPA."Genes Dev. 23:2400-2404(2009) [PubMed] [Europe PMC] [Abstract]
  24. "The annealing helicase SMARCAL1 maintains genome integrity at stalled replication forks."Genes Dev. 23:2405-2414(2009) [PubMed] [Europe PMC] [Abstract]
  25. "The SIOD disorder protein SMARCAL1 is an RPA-interacting protein involved in replication fork restart."Genes Dev. 23:2415-2425(2009) [PubMed] [Europe PMC] [Abstract]
  26. "An alternative form of replication protein a prevents viral replication in vitro."J. Biol. Chem. 284:5324-5331(2009) [PubMed] [Europe PMC] [Abstract]
  27. "An alternative form of replication protein a expressed in normal human tissues supports DNA repair."J. Biol. Chem. 285:4788-4797(2010) [PubMed] [Europe PMC] [Abstract]
  28. "A PP4 phosphatase complex dephosphorylates RPA2 to facilitate DNA repair via homologous recombination."Nat. Struct. Mol. Biol. 17:365-372(2010) [PubMed] [Europe PMC] [Abstract]
  29. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
  30. "Initial characterization of the human central proteome."BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
  31. "E3 ligase RFWD3 participates in replication checkpoint control."J. Biol. Chem. 286:22308-22313(2011) [PubMed] [Europe PMC] [Abstract]
  32. "RING finger and WD repeat domain 3 (RFWD3) associates with replication protein A (RPA) and facilitates RPA-mediated DNA damage response."J. Biol. Chem. 286:22314-22322(2011) [PubMed] [Europe PMC] [Abstract]
  33. "DNA-PK-dependent RPA2 hyperphosphorylation facilitates DNA repair and suppresses sister chromatid exchange."PLoS ONE 6:E21424-E21424(2011) [PubMed] [Europe PMC] [Abstract]
  34. "Translational regulation of RPA2 via internal ribosomal entry site and by eIF3a."Carcinogenesis 34:1224-1231(2013) [PubMed] [Europe PMC] [Abstract]
  35. "PRP19 transforms into a sensor of RPA-ssDNA after DNA damage and drives ATR activation via a ubiquitin-mediated circuitry."Mol. Cell 53:235-246(2014) [PubMed] [Europe PMC] [Abstract]
  36. "The crystal structure of the complex of replication protein A subunits RPA32 and RPA14 reveals a mechanism for single-stranded DNA binding."EMBO J. 18:4498-4504(1999) [PubMed] [Europe PMC] [Abstract]
  37. "Structural basis for the recognition of DNA repair proteins UNG2, XPA, and RAD52 by replication factor RPA."Cell 103:449-456(2000) [PubMed] [Europe PMC] [Abstract]
  38. "Structure of the RPA trimerization core and its role in the multistep DNA-binding mechanism of RPA."EMBO J. 21:1855-1863(2002) [PubMed] [Europe PMC] [Abstract]