≡

Replication Protein A1 (RPA1)

[Edit]

HSSB; REPA1; RF-A; RP-A; RPA70; Replication protein A 70 kDa DNA-binding subunit; Single-stranded DNA-binding protein

Replication Protein A1 (RPA1)
CCC H217
HGNC 10289
MGI 1915525
PubMed 21901111
RGD 1307376
UniProt P27694
Wiki RPA1
Replication protein A (RPA) is a 3-subunit single-stranded DNA (ssDNA)-binding protein that has been isolated from human cells and found to be essential for in vitro replication of the papovavirus SV40. The human cDNA directed production in E. coli of a 70-kD protein that reacted with a monoclonal antibody directed against the 70-kD subunit of the human protein. The recombinant subunit, purified from bacteria, exhibited single-stranded DNA-binding activity comparable to that of the complete RPA complex. It could substitute for the complete complex in stimulating the activity of DNA polymerase alpha-primase, but could not substitute for the complete complex in SV40 DNA replication in vitro, suggesting an important functional role for the other subunits.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPH217Hu01 Recombinant Replication Protein A1 (RPA1) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies MAH217Hu21 Monoclonal Antibody to Replication Protein A1 (RPA1) WB; IHC; ICC; IP.
PAH217Hu01 Polyclonal Antibody to Replication Protein A1 (RPA1) WB; IHC; ICC; IP.
Assay Kits SEH217Hu ELISA Kit for Replication Protein A1 (RPA1) Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Replication Protein A1 (RPA1) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Replication Protein A1 (RPA1) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Replication Protein A1 (RPA1) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Replication Protein A1 (RPA1) CLIA Kit Customized Service Offer
n/a ELISA Kit for Replication Protein A1 (RPA1) ELISA Kit Customized Service Offer

Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPH217Ra01 Recombinant Replication Protein A1 (RPA1) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies MAH217Ra21 Monoclonal Antibody to Replication Protein A1 (RPA1) WB; IHC; ICC; IP.
PAH217Ra01 Polyclonal Antibody to Replication Protein A1 (RPA1) WB; IHC; ICC; IP.
Assay Kits SEH217Ra ELISA Kit for Replication Protein A1 (RPA1) Enzyme-linked immunosorbent assay for Antigen Detection.
  1. "Characterization of a cDNA encoding the 70-kDa single-stranded DNA-binding subunit of human replication protein A and the role of the protein in DNA replication."J. Biol. Chem. 266:12090-12098(1991) [PubMed] [Europe PMC] [Abstract]
  2. "Type I human complement C2 deficiency. A 28-base pair gene deletion causes skipping of exon 6 during RNA splicing."J. Biol. Chem. 268:2268-2268(1993) [PubMed] [Europe PMC] [Abstract]
  3. "Complete sequencing and characterization of 21,243 full-length human cDNAs." Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]