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Phosphohistidine Phosphatase 1 (PHPT1)

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PHP14; Phosphohistidine Phosphatase 14kDa; Sex-Regulated Protein Janus-a; Protein janus-A homolog

Phosphohistidine Phosphatase 1 (PHPT1)
CCC H413
HGNC 30033
MGI 1922704
PubMed 19380421
RGD 1311355
UniProt Q9NRX4
Wiki PHPT1
PHPT1 is an EDTA-insensitive phosphohistidine phosphatase that catalyzes the dephosphorylation of phosphopeptide I . PHPT1 gene contains 3 exons.
The deduced 125-amino acid PHPT1 protein migrated as a single, approximately 14 kD band by SDS-PAGE. Northern blot analysis detected a 0.6-kb transcript, with highest expression in heart and skeletal muscle and lower expression in several other tissues including pancreas, liver, and kidney.recombinant PHPT1 overexpressed and purified from bacteria displayed specific phosphohistidine phosphatase activity toward phosphopeptide I. No activity was detected towards phosphoserine, phosphothreonine, or phosphotyrosine peptides.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPH413Hu01 Recombinant Phosphohistidine Phosphatase 1 (PHPT1) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAH413Hu01 Polyclonal Antibody to Phosphohistidine Phosphatase 1 (PHPT1) WB,IHC,ICC/IF
LAH413Hu71 Biotin-Linked Polyclonal Antibody to Phosphohistidine Phosphatase 1 (PHPT1) WB; IHC; ICC.
LAH413Hu81 FITC-Linked Polyclonal Antibody to Phosphohistidine Phosphatase 1 (PHPT1) WB; IHC; ICC; IF.
Assay Kits SEH413Hu ELISA Kit for Phosphohistidine Phosphatase 1 (PHPT1) Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPH413Mu01 Recombinant Phosphohistidine Phosphatase 1 (PHPT1) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAH413Mu01 Polyclonal Antibody to Phosphohistidine Phosphatase 1 (PHPT1) WB; IHC; ICC; IP.
LAH413Mu71 Biotin-Linked Polyclonal Antibody to Phosphohistidine Phosphatase 1 (PHPT1) WB; IHC; ICC.
Assay Kits n/a CLIA Kit for Phosphohistidine Phosphatase 1 (PHPT1) CLIA Kit Customized Service Offer
n/a ELISA Kit for Phosphohistidine Phosphatase 1 (PHPT1) ELISA Kit Customized Service Offer

Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Phosphohistidine Phosphatase 1 (PHPT1) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Phosphohistidine Phosphatase 1 (PHPT1) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Phosphohistidine Phosphatase 1 (PHPT1) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Phosphohistidine Phosphatase 1 (PHPT1) CLIA Kit Customized Service Offer
n/a ELISA Kit for Phosphohistidine Phosphatase 1 (PHPT1) ELISA Kit Customized Service Offer
  1. "Identification and characterization of a mammalian 14-kDa phosphohistidine phosphatase."Eur. J. Biochem. 269:5016-5023(2002) [PubMed] [Europe PMC] [Abstract]
  2. "Gene expression profiling in the human hypothalamus-pituitary-adrenal axis and full-length cDNA cloning." Proc. Natl. Acad. Sci. U.S.A. 97:9543-9548(2000) [PubMed] [Europe PMC] [Abstract]
  3. "Identification of the human crooked neck gene by comparative gene identification."Biochim. Biophys. Acta 1517:449-454(2001) [PubMed] [Europe PMC] [Abstract]
  4. "Towards a catalog of human genes and proteins: sequencing and analysis of 500 novel complete protein coding human cDNAs." Genome Res. 11:422-435(2001) [PubMed] [Europe PMC] [Abstract]
  5. "DNA sequence and analysis of human chromosome 9." Nature 429:369-374(2004) [PubMed] [Europe PMC] [Abstract]
  6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
  7. "Mutational study of human phosphohistidine phosphatase: effect on enzymatic activity."Biochem. Biophys. Res. Commun. 337:887-891(2005) [PubMed] [Europe PMC] [Abstract]
  8. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
  9. "Initial characterization of the human central proteome."BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
  10. "Comparative large-scale characterisation of plant vs. mammal proteins reveals similar and idiosyncratic N-alpha acetylation features."Mol. Cell. Proteomics 11:M111.015131-M111.015131(2012) [PubMed] [Europe PMC] [Abstract]
  11. "N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]
  12. "First structure of a eukaryotic phosphohistidine phosphatase."J. Biol. Chem. 281:33830-33834(2006) [PubMed] [Europe PMC] [Abstract]
  13. "Solution structure and catalytic mechanism of human protein histidine phosphatase 1."Biochem. J. 418:337-344(2009) [PubMed] [Europe PMC] [Abstract]
  14. "Crystal structure of human phosphohistidine phosphatase. Northeast structural genomics consortium target HR1409."Submitted (JAN-2007) to the PDB data bank