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p21 Protein Activated Kinase 1 (PAK1)

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PAKalpha; p65-PAK; p21/Cdc42/Rac1-Activated Kinase 1; Serine/threonine-protein kinase PAK 1

p21 Protein Activated Kinase 1 (PAK1)
CCC H469
HGNC 8590
MGI 1339975
PubMed 8805275
RGD 3250
UniProt Q13153
Wiki PAK1
PAK proteins are critical effectors that link RhoGTPases to cytoskeleton reorganization and nuclear signaling. PAK proteins, a family of serine/threonine p21-activating kinases, include PAK1, PAK2, PAK3 and PAK4. These proteins serve as targets for the small GTP binding proteins Cdc42 and Rac and have been implicated in a wide range of biological activities. PAK1 regulates cell motility and morphology.
PAK1 is a member of a family of serine/threonine kinases related to yeast Ste20. The human PAK1 gene encodes a 545-amino acid polypeptide that is 98% identical to rat PAK1 and 52% identical to yeast Ste20. Activity of PAK1 is induced by coexpression with RAC1 or CDC42. PAK is believed to act directly on the JNK1 MAP kinase pathway.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPH469Hu01 Recombinant p21 Protein Activated Kinase 1 (PAK1) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAH469Hu01 Polyclonal Antibody to p21 Protein Activated Kinase 1 (PAK1) WB,IHC
Assay Kits SEH469Hu ELISA Kit for p21 Protein Activated Kinase 1 (PAK1) Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant p21 Protein Activated Kinase 1 (PAK1) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to p21 Protein Activated Kinase 1 (PAK1) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to p21 Protein Activated Kinase 1 (PAK1) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for p21 Protein Activated Kinase 1 (PAK1) CLIA Kit Customized Service Offer
n/a ELISA Kit for p21 Protein Activated Kinase 1 (PAK1) ELISA Kit Customized Service Offer

Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant p21 Protein Activated Kinase 1 (PAK1) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to p21 Protein Activated Kinase 1 (PAK1) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to p21 Protein Activated Kinase 1 (PAK1) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for p21 Protein Activated Kinase 1 (PAK1) CLIA Kit Customized Service Offer
n/a ELISA Kit for p21 Protein Activated Kinase 1 (PAK1) ELISA Kit Customized Service Offer
  1. "Human Ste20 homologue hPAK1 links GTPases to the JNK MAP kinase pathway."Curr. Biol. 6:598-605(1996) [PubMed] [Europe PMC] [Abstract]
  2. "Human p21-activated kinase (Pak1) regulates actin organization in mammalian cells."Curr. Biol. 7:202-210(1997) [PubMed] [Europe PMC] [Abstract]
  3. "Human chromosome 11 DNA sequence and analysis including novel gene identification." Nature 440:497-500(2006) [PubMed] [Europe PMC] [Abstract]
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
  5. "Expression of constitutively active alpha-PAK reveals effects of the kinase on actin and focal complexes."Mol. Cell. Biol. 17:1129-1143(1997) [PubMed] [Europe PMC] [Abstract]
  6. "Differential effects of PAK1-activating mutations reveal activity-dependent and -independent effects on cytoskeletal regulation."J. Biol. Chem. 273:28191-28198(1998) [PubMed] [Europe PMC] [Abstract]
  7. "A conserved negative regulatory region in alphaPAK: inhibition of PAK kinases reveals their morphological roles downstream of Cdc42 and Rac1."Mol. Cell. Biol. 18:2153-2163(1998) [PubMed] [Europe PMC] [Abstract]
  8. "Identification of Grb4/Nckbeta, a src homology 2 and 3 domain-containing adapter protein having similar binding and biological properties to Nck."J. Biol. Chem. 274:5542-5549(1999) [PubMed] [Europe PMC] [Abstract]
  9. "Identification of a central phosphorylation site in p21-activated kinase regulating autoinhibition and kinase activity."J. Biol. Chem. 274:32565-32573(1999) [PubMed] [Europe PMC] [Abstract]
  10. "p21-activated kinase (PAK1) is phosphorylated and activated by 3-phosphoinositide-dependent kinase-1 (PDK1)."J. Biol. Chem. 275:41201-41209(2000) [PubMed] [Europe PMC] [Abstract]
  11. "Interaction between active Pak1 and Raf-1 is necessary for phosphorylation and activation of Raf-1."J. Biol. Chem. 277:4395-4405(2002) [PubMed] [Europe PMC] [Abstract]
  12. "Pak1 kinase homodimers are autoinhibited in trans and dissociated upon activation by Cdc42 and Rac1."Mol. Cell 9:73-83(2002) [PubMed] [Europe PMC] [Abstract]
  13. "The C-terminal kinase domain of the p34cdc2-related PITSLRE protein kinase (p110C) associates with p21-activated kinase 1 and inhibits its activity during anoikis."J. Biol. Chem. 278:20029-20036(2003) [PubMed] [Europe PMC] [Abstract]
  14. "PAK1 phosphorylation of MEK1 regulates fibronectin-stimulated MAPK activation."J. Cell Biol. 162:281-291(2003) [PubMed] [Europe PMC] [Abstract]
  15. "Akt phosphorylation of serine 21 on Pak1 modulates Nck binding and cell migration."Mol. Cell. Biol. 23:8058-8069(2003) [PubMed] [Europe PMC] [Abstract]
  16. "The Down syndrome cell adhesion molecule (DSCAM) interacts with and activates Pak."J. Biol. Chem. 279:32824-32831(2004) [PubMed] [Europe PMC] [Abstract]
  17. "Pak1 phosphorylation of snail, a master regulator of epithelial-to-mesenchyme transition, modulates snail's subcellular localization and functions."Cancer Res. 65:3179-3184(2005) [PubMed] [Europe PMC] [Abstract]
  18. "Integrin engagement differentially modulates epithelial cell motility by RhoA/ROCK and PAK1."J. Biol. Chem. 280:10624-10635(2005) [PubMed] [Europe PMC] [Abstract]
  19. "Essential role of CIB1 in regulating PAK1 activation and cell migration."J. Cell Biol. 170:465-476(2005) [PubMed] [Europe PMC] [Abstract]
  20. "p21-activated kinase 1 regulates microtubule dynamics by phosphorylating tubulin cofactor B."Mol. Cell. Biol. 25:3726-3736(2005) [PubMed] [Europe PMC] [Abstract]
  21. "A probability-based approach for high-throughput protein phosphorylation analysis and site localization."Nat. Biotechnol. 24:1285-1292(2006) [PubMed] [Europe PMC] [Abstract]
  22. "CRIPak, a novel endogenous Pak1 inhibitor."Oncogene 25:1311-1319(2006) [PubMed] [Europe PMC] [Abstract]
  23. "JAK2 tyrosine kinase phosphorylates PAK1 and regulates PAK1 activity and functions."J. Biol. Chem. 282:30985-30996(2007) [PubMed] [Europe PMC] [Abstract]
  24. "Identification of phosphorylation sites in betaPIX and PAK1."J. Cell Sci. 120:3911-3918(2007) [PubMed] [Europe PMC] [Abstract]
  25. "Affixin activates Rac1 via betaPIX in C2C12 myoblast."FEBS Lett. 582:1189-1196(2008) [PubMed] [Europe PMC] [Abstract]
  26. "Scrib regulates PAK activity during the cell migration process."Hum. Mol. Genet. 17:3552-3565(2008) [PubMed] [Europe PMC] [Abstract]
  27. "A quantitative atlas of mitotic phosphorylation."Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
  28. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
  29. "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
  30. "Initial characterization of the human central proteome."BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
  31. "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
  32. "Synapses of amphids defective (SAD-A) kinase promotes glucose-stimulated insulin secretion through activation of p21-activated kinase (PAK1) in pancreatic beta-Cells."J. Biol. Chem. 287:26435-26444(2012) [PubMed] [Europe PMC] [Abstract]
  33. "N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]
  34. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]
  35. "A novel splice variant of calcium and integrin-binding protein 1 mediates protein kinase D2-stimulated tumour growth by regulating angiogenesis."Oncogene 33:1167-1180(2014) [PubMed] [Europe PMC] [Abstract]
  36. "Beta-arrestin-dependent activation of the cofilin pathway is required for the scavenging activity of the atypical chemokine receptor D6."Sci. Signal. 6:RA30-RA30(2013) [PubMed] [Europe PMC] [Abstract]
  37. "Structure of PAK1 in an autoinhibited conformation reveals a multistage activation switch."Cell 102:387-397(2000) [PubMed] [Europe PMC] [Abstract]
  38. "Structural analysis of the SH3 domain of beta-PIX and its interaction with alpha-p21 activated kinase (PAK)."Biochemistry 44:10977-10983(2005) [PubMed] [Europe PMC] [Abstract]
  39. "The active conformation of the PAK1 kinase domain."Structure 13:769-778(2005) [PubMed] [Europe PMC] [Abstract]
  40. "Structural insights into the autoactivation mechanism of p21-activated protein kinase."Structure 19:1752-1761(2011) [PubMed] [Europe PMC] [Abstract]