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N-glycanase is a highly conserved enzyme that catalyzes deglycosylation of misfolded N-linked glycoproteins by cleaving the glycan chain before the proteins are degraded by the proteasome.
The deduced 654-amino acid human protein contains an N-terminal PUB/PUG protein-protein interaction domain and a central transglutaminase-like motif containing the catalytic triad of the active site. Mouse and human NGLY1 share 98% identity in these 2 domains. Northern blot analysis detected Ngly1 expression in all mouse tissues examined, with highest expression in testis.In yeast, Png1 was expressed in both the cytoplasm and nucleus.
Organism species: Homo sapiens (Human)
| CATALOG NO. | PRODUCT NAME | APPLICATIONS | |
| Proteins | n/a | Recombinant N-Glycanase 1 (NGLY1) | Recombinant Protein Customized Service Offer |
| Antibodies | n/a | Monoclonal Antibody to N-Glycanase 1 (NGLY1) | Monoclonal Antibody Customized Service Offer |
| n/a | Polyclonal Antibody to N-Glycanase 1 (NGLY1) | Polyclonal Antibody Customized Service Offer | |
| Assay Kits | n/a | CLIA Kit for N-Glycanase 1 (NGLY1) | CLIA Kit Customized Service Offer |
| n/a | ELISA Kit for N-Glycanase 1 (NGLY1) | ELISA Kit Customized Service Offer |
Organism species: Mus musculus (Mouse)
| CATALOG NO. | PRODUCT NAME | APPLICATIONS | |
| Proteins | n/a | Recombinant N-Glycanase 1 (NGLY1) | Recombinant Protein Customized Service Offer |
| Antibodies | n/a | Monoclonal Antibody to N-Glycanase 1 (NGLY1) | Monoclonal Antibody Customized Service Offer |
| n/a | Polyclonal Antibody to N-Glycanase 1 (NGLY1) | Polyclonal Antibody Customized Service Offer | |
| Assay Kits | n/a | CLIA Kit for N-Glycanase 1 (NGLY1) | CLIA Kit Customized Service Offer |
| n/a | ELISA Kit for N-Glycanase 1 (NGLY1) | ELISA Kit Customized Service Offer |
Organism species: Rattus norvegicus (Rat)
| CATALOG NO. | PRODUCT NAME | APPLICATIONS | |
| Proteins | n/a | Recombinant N-Glycanase 1 (NGLY1) | Recombinant Protein Customized Service Offer |
| Antibodies | n/a | Monoclonal Antibody to N-Glycanase 1 (NGLY1) | Monoclonal Antibody Customized Service Offer |
| n/a | Polyclonal Antibody to N-Glycanase 1 (NGLY1) | Polyclonal Antibody Customized Service Offer | |
| Assay Kits | n/a | CLIA Kit for N-Glycanase 1 (NGLY1) | CLIA Kit Customized Service Offer |
| n/a | ELISA Kit for N-Glycanase 1 (NGLY1) | ELISA Kit Customized Service Offer |
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- "A glycosylated type I membrane protein becomes cytosolic when peptide: N-glycanase is compromised."EMBO J. 23:650-658(2004) [PubMed] [Europe PMC] [Abstract]
- "A complex between peptide:N-glycanase and two proteasome-linked proteins suggests a mechanism for the degradation of misfolded glycoproteins."Proc. Natl. Acad. Sci. U.S.A. 101:13774-13779(2004) [PubMed] [Europe PMC] [Abstract]
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- "The retrotranslocation protein derlin-1 binds peptide:N-glycanase to the endoplasmic reticulum."Mol. Biol. Cell 16:4584-4594(2005) [PubMed] [Europe PMC] [Abstract]
- "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
- "Clinical application of exome sequencing in undiagnosed genetic conditions."J. Med. Genet. 49:353-361(2012) [PubMed] [Europe PMC] [Abstract]
- "Mutations in NGLY1 cause an inherited disorder of the endoplasmic reticulum-associated degradation pathway." Genet. Med. 16:751-758(2014) [PubMed] [Europe PMC] [Abstract]
- "The PUB domain functions as a p97 binding module in human peptide N-glycanase."J. Biol. Chem. 281:25502-25508(2006) [PubMed] [Europe PMC] [Abstract]