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Nexilin (NEXN)

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NELIN; F Actin Binding Protein

Nexilin (NEXN)
CCC H548
HGNC 29557
MGI 1916060
PubMed 19881492
RGD 708354
UniProt Q0ZGT2
The name nexilin came from the Latin word nexilis, meaning 'bound together.' Full-length b-nexilin contains an N-terminal F-actin-binding domain (ABD), followed by a spacer, a coiled-coil region, a second ABD, and a long C-terminal tail. In comparison, s-nexilin lacks the N-terminal ABD and has an insertion in the C-terminal tail. Immunofluorescence microscopy revealed that both b-nexilin and s-nexilin colocalized with F-actin at focal contacts at the ends of stress fibers.
The nexilins did not localize to cell-cell junctions. Western blot analysis detected nexilin proteins with apparent molecular masses above 90 kD in rat brain, testis, and spleen and in cultured rodent fibroblasts. Neither protein was detected in liver, kidney, or epithelial cell lines.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPH548Hu01 Recombinant Nexilin (NEXN) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAH548Hu01 Polyclonal Antibody to Nexilin (NEXN) WB; IHC; ICC; IP.
MAH548Hu21 Monoclonal Antibody to Nexilin (NEXN) WB; IHC; ICC; IP.
MAH548Hu22 Monoclonal Antibody to Nexilin (NEXN) WB; IHC; ICC; IP.
MAH548Hu23 Monoclonal Antibody to Nexilin (NEXN) WB; IHC; ICC; IP.
MAH548Hu24 Monoclonal Antibody to Nexilin (NEXN) WB; IHC; ICC; IP.
Assay Kits SEH548Hu ELISA Kit for Nexilin (NEXN) Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPH548Mu01 Recombinant Nexilin (NEXN) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAH548Mu01 Polyclonal Antibody to Nexilin (NEXN) WB; IHC; ICC; IP.
Assay Kits SEH548Mu ELISA Kit for Nexilin (NEXN) Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPH548Ra01 Recombinant Nexilin (NEXN) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAH548Ra01 Polyclonal Antibody to Nexilin (NEXN) WB,IHC
Assay Kits SEH548Ra ELISA Kit for Nexilin (NEXN) Enzyme-linked immunosorbent assay for Antigen Detection.
  1. "Nexilin mutations destabilize cardiac Z-disks and lead to dilated cardiomyopathy."Nat. Med. 15:1281-1288(2009) [PubMed] [Europe PMC] [Abstract]
  2. "Complete sequencing and characterization of 21,243 full-length human cDNAs." Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract].
  3. "The DNA sequence and biological annotation of human chromosome 1." Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract]
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract].
  5. "Molecular cloning of NELIN, a putative human cytoskeleton regulation gene."Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao 33:19-24(2001) [PubMed] [Europe PMC] [Abstract].
  6. "Muscle autoantigens in thyroid associated ophthalmopathy: the limits of molecular genetics."J. Endocrinol. Invest. 16:533-540(1993) [PubMed] [Europe PMC] [Abstract]
  7. "NELIN, a new F-actin associated protein, stimulates HeLa cell migration and adhesion."Biochem. Biophys. Res. Commun. 330:1127-1131(2005) [PubMed] [Europe PMC] [Abstract]
  8. "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract]
  9. "Improved titanium dioxide enrichment of phosphopeptides from HeLa cells and high confident phosphopeptide identification by cross-validation of MS/MS and MS/MS/MS spectra."J. Proteome Res. 6:4150-4162(2007) [PubMed] [Europe PMC] [Abstract]
  10. "A quantitative atlas of mitotic phosphorylation."Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
  11. "Large-scale phosphoproteome analysis of human liver tissue by enrichment and fractionation of phosphopeptides with strong anion exchange chromatography."Proteomics 8:1346-1361(2008) [PubMed] [Europe PMC] [Abstract]
  12. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
  13. "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
  14. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]
  15. "Mutations in NEXN, a Z-disc gene, are associated with hypertrophic cardiomyopathy."Am. J. Hum. Genet. 87:687-693(2010) [PubMed] [Europe PMC] [Abstract]