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Methyltransferase Like Protein 3 (METTL3)

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M6A; MT-A70; Spo8; N6-adenosine-methyltransferase 70 kDa subunit

Methyltransferase Like Protein 3 (METTL3)
CCC H659
HGNC 17563
MGI 1927165
PubMed 24316715
RGD 1311750
UniProt Q86U44
Wiki METTL3
The methylation of internal adenosine residues in eukaryotic mRNA, forming N6-methyladenosine (m6A), is catalyzed by a complex multicomponent enzyme, of which METTL3 is the critical S-adenosyl-L-methionine-binding subunit.
The deduced 580-amino acid protein has a calculated molecular mass of 65 kD, close to the purification product molecular mass of 70 kD. METTL3 contains 2 putative nuclear localization signals and 2 consensus methylation motifs. Northern blot analysis detected 2.0-kb and 3.0-kb transcripts in all tissues examined. METTL3 shares 54% amino acid identity with the C-terminal half of S. cerevisiae Spo8, which was subsequently renamed Ime4. Immunofluorescnce localized METTL3 to speckles in interphase HeLa cell nuclei.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Methyltransferase Like Protein 3 (METTL3) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Methyltransferase Like Protein 3 (METTL3) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Methyltransferase Like Protein 3 (METTL3) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Methyltransferase Like Protein 3 (METTL3) CLIA Kit Customized Service Offer
n/a ELISA Kit for Methyltransferase Like Protein 3 (METTL3) ELISA Kit Customized Service Offer

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Methyltransferase Like Protein 3 (METTL3) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Methyltransferase Like Protein 3 (METTL3) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Methyltransferase Like Protein 3 (METTL3) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Methyltransferase Like Protein 3 (METTL3) CLIA Kit Customized Service Offer
n/a ELISA Kit for Methyltransferase Like Protein 3 (METTL3) ELISA Kit Customized Service Offer

Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Methyltransferase Like Protein 3 (METTL3) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Methyltransferase Like Protein 3 (METTL3) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Methyltransferase Like Protein 3 (METTL3) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Methyltransferase Like Protein 3 (METTL3) CLIA Kit Customized Service Offer
n/a ELISA Kit for Methyltransferase Like Protein 3 (METTL3) ELISA Kit Customized Service Offer
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  2. "The DNA sequence and analysis of human chromosome 14." Nature 421:601-607(2003) [PubMed] [Europe PMC] [Abstract]
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
  4. "A probability-based approach for high-throughput protein phosphorylation analysis and site localization."Nat. Biotechnol. 24:1285-1292(2006) [PubMed] [Europe PMC] [Abstract]
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  7. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
  8. "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
  9. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
  10. "Initial characterization of the human central proteome."BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
  11. "Topology of the human and mouse m6A RNA methylomes revealed by m6A-seq."Nature 485:201-206(2012) [PubMed] [Europe PMC] [Abstract]
  12. "Mammalian WTAP is a regulatory subunit of the RNA N6-methyladenosine methyltransferase." Cell Res. 24:177-189(2014) [PubMed] [Europe PMC] [Abstract]
  13. "Perturbation of m6A writers reveals two distinct classes of mRNA methylation at internal and 5' sites."Cell Rep. 8:284-296(2014) [PubMed] [Europe PMC] [Abstract]
  14. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]
  15. "N-methyladenosine-dependent regulation of messenger RNA stability."Nature 505:117-120(2014) [PubMed] [Europe PMC] [Abstract]
  16. "N(6)-methyladenosine-dependent RNA structural switches regulate RNA-protein interactions."Nature 518:560-564(2015) [PubMed] [Europe PMC] [Abstract]
  17. "N6-methyladenosine marks primary microRNAs for processing."Nature 519:482-485(2015) [PubMed] [Europe PMC] [Abstract]