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Methionyl Aminopeptidase 2 (METAP2)

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MNPEP; p67; p67eIF2; Peptidase M; Initiation factor 2-associated 67 kDa glycoprotein

Methionyl Aminopeptidase 2 (METAP2)
CCC H663
HGNC 16672
MGI 1929701
PubMed 23161775
RGD 70995
UniProt P50579
Wiki METAP2
METAP2 is a member of the methionyl aminopeptidase family and encodes a protein that binds 2 cobalt or manganese ions. This protein functions both by protecting the alpha subunit of eukaryotic initiation factor 2 from inhibitory phosphorylation and by removing the amino-terminal methionine residue from nascent protein. Increased expression of this gene is associated with various forms of cancer and the anti-cancer drugs fumagillin and ovalicin inhibit the protein by irreversibly binding to its active site. A pseudogene of this gene is located on chromosome 2.Sequence analysis showed that the human gene encodes a 478-amino acid polypeptide that shares 92% identity with rat p67. Northern blot analysis revealed that human p67 is expressed as a 2.1-kb transcript in all tissues tested.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPH663Hu01 Recombinant Methionyl Aminopeptidase 2 (METAP2) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAH663Hu01 Polyclonal Antibody to Methionyl Aminopeptidase 2 (METAP2) WB; IHC; ICC; IP.
MAH663Hu21 Monoclonal Antibody to Methionyl Aminopeptidase 2 (METAP2) WB; IHC; ICC; IP.
Assay Kits SEH663Hu ELISA Kit for Methionyl Aminopeptidase 2 (METAP2) Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPH663Mu01 Recombinant Methionyl Aminopeptidase 2 (METAP2) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAH663Mu01 Polyclonal Antibody to Methionyl Aminopeptidase 2 (METAP2) WB; IHC; ICC; IP.
Assay Kits SEH663Mu ELISA Kit for Methionyl Aminopeptidase 2 (METAP2) Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Methionyl Aminopeptidase 2 (METAP2) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Methionyl Aminopeptidase 2 (METAP2) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Methionyl Aminopeptidase 2 (METAP2) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Methionyl Aminopeptidase 2 (METAP2) CLIA Kit Customized Service Offer
n/a ELISA Kit for Methionyl Aminopeptidase 2 (METAP2) ELISA Kit Customized Service Offer
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  2. "Molecular cloning of a human complementary DNA encoding an initiation factor 2-associated protein (p67)."Biochim. Biophys. Acta 1260:333-336(1995) [PubMed] [Europe PMC] [Abstract]
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  4. "The finished DNA sequence of human chromosome 12." Nature 440:346-351(2006) [PubMed] [Europe PMC] [Abstract]
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  6. "Glycosylation of eukaryotic peptide chain initiation factor 2 (eIF-2)-associated 67-kDa polypeptide (p67) and its possible role in the inhibition of eIF-2 kinase-catalyzed phosphorylation of the eIF-2 alpha-subunit."J. Biol. Chem. 264:20620-20624(1989) [PubMed] [Europe PMC] [Abstract]
  7. "Physiologically relevant metal cofactor for methionine aminopeptidase-2 is manganese."Biochemistry 42:5035-5042(2003) [PubMed] [Europe PMC] [Abstract]
  8. "A quantitative atlas of mitotic phosphorylation."Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
  9. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
  10. "Protein N-terminal processing: substrate specificity of Escherichia coli and human methionine aminopeptidases."Biochemistry 49:5588-5599(2010) [PubMed] [Europe PMC] [Abstract]
  11. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
  12. "Localization and function of a eukaryotic-initiation-factor-2-associated 67-kDa glycoprotein."World J. Biol. Chem. 1:313-320(2010) [PubMed] [Europe PMC] [Abstract]
  13. "Initial characterization of the human central proteome."BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
  14. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]
  15. "Structure of human methionine aminopeptidase-2 complexed with fumagillin."Science 282:1324-1327(1998) [PubMed] [Europe PMC] [Abstract]
  16. "Proteomics-based target identification: bengamides as a new class of methionine aminopeptidase inhibitors."J. Biol. Chem. 278:52964-52971(2003) [PubMed] [Europe PMC] [Abstract]
  17. "3-amino-2-hydroxyamides and related compounds as inhibitors of methionine aminopeptidase-2."Bioorg. Med. Chem. Lett. 14:865-868(2004) [PubMed] [Europe PMC] [Abstract]
  18. "4-aryl-1,2,3-triazole: a novel template for a reversible methionine aminopeptidase 2 inhibitor, optimized to inhibit angiogenesis in vivo." J. Med. Chem. 48:5644-5647(2005) [PubMed] [Europe PMC] [Abstract]
  19. "Human methionine aminopeptidase type 2 in complex with L- and D-methionine."Bioorg. Med. Chem. Lett. 16:2580-2583(2006) [PubMed] [Europe PMC] [Abstract]
  20. "Lead optimization of methionine aminopeptidase-2 (MetAP2) inhibitors containing sulfonamides of 5,6-disubstituted anthranilic acids."Bioorg. Med. Chem. Lett. 17:2817-2822(2007) [PubMed] [Europe PMC] [Abstract]
  21. "Highly potent inhibitors of methionine aminopeptidase-2 based on a 1,2,4-triazole pharmacophore."J. Med. Chem. 50:3777-3785(2007) [PubMed] [Europe PMC] [Abstract]