Lectin, Mannose Binding 2 Like Protien (LMAN2L)

VIPL is ubiquitously expressed. VIPL distributed with the membrane pellet of fractionated transfected human embryonic kidney cells. Confocal immunofluorescence microscopy localized VIPL to the ER of HepG2 and all other human cell lines examined. Endoglycosidase treatment indicated that the N-glycan of VIPL is a high-mannose-type carbohydrate.The deduced 348-amino acid protein contains an N-terminal signal peptide, followed by a lectin-type carbohydrate recognition domain (CARD), a transmembrane domain, and a C-terminal endoplasmic reticulum (ER) retrieval motif. The CRD has conserved cysteines and key residues required for Ca(2+) and sugar binding, as well as an N-glycosylation site. VIPL shares 35% and 58% amino acid identity with ERGIC53 (LMAN1) and VIP36 (LMAN2).