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Phosphopantothenoylcysteine Synthetase (PPCS)

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COAB; Phosphopantothenate--cysteine ligase

Phosphopantothenoylcysteine Synthetase (PPCS)
CCC H742
HGNC 25686
MGI 1915237
PubMed 22113182
UniProt Q9HAB8
Biosynthesis of coenzyme A (CoA) from pantothenic acid (vitamin B5) is an essential universal pathway in prokaryotes and eukaryotes. PPCS (EC 6.3.2.5), one of the last enzymes in this pathway, converts phosphopantothenate to phosphopantothenoylcysteine.
Daugherty et al. (2002) showed that recombinant PPCS functioned within the CoA synthetic pathway. PPCS used ATP for the activation of substrate in the ligation reaction 4 times more efficiently than CTP. Daugherty et al. (2002) verified the function of PPCS by complementation in E. coli. Incubation of PPCS, PPCDC, and the bifunctional enzyme COASY with the necessary substrates and cofactors reconstituted the 4-step biochemical transformation of phosphopantothenate to CoA.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Phosphopantothenoylcysteine Synthetase (PPCS) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Phosphopantothenoylcysteine Synthetase (PPCS) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Phosphopantothenoylcysteine Synthetase (PPCS) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Phosphopantothenoylcysteine Synthetase (PPCS) CLIA Kit Customized Service Offer
n/a ELISA Kit for Phosphopantothenoylcysteine Synthetase (PPCS) ELISA Kit Customized Service Offer

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Phosphopantothenoylcysteine Synthetase (PPCS) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Phosphopantothenoylcysteine Synthetase (PPCS) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Phosphopantothenoylcysteine Synthetase (PPCS) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Phosphopantothenoylcysteine Synthetase (PPCS) CLIA Kit Customized Service Offer
n/a ELISA Kit for Phosphopantothenoylcysteine Synthetase (PPCS) ELISA Kit Customized Service Offer
  1. "Complete sequencing and characterization of 21,243 full-length human cDNAs." Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
  2. "The DNA sequence and biological annotation of human chromosome 1." Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract]
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
  4. "Complete reconstitution of the human coenzyme A biosynthetic pathway via comparative genomics."J. Biol. Chem. 277:21431-21439(2002) [PubMed] [Europe PMC] [Abstract]
  5. "Initial characterization of the human central proteome."BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
  6. "Comparative large-scale characterisation of plant vs. mammal proteins reveals similar and idiosyncratic N-alpha acetylation features."Mol. Cell. Proteomics 11:M111.015131-M111.015131(2012) [PubMed] [Europe PMC] [Abstract]
  7. "N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]
  8. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]
  9. "Structure of human phosphopantothenoylcysteine synthetase at 2.3 A resolution."Structure 11:927-936(2003) [PubMed] [Europe PMC] [Abstract]