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Kinetochore Associated Protein 2 (KNTC2)

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NDC80; HEC; HEC1; TID3; hsNDC80; Retinoblastoma-associated protein HEC; Kinetochore Protein NDC80 Homolog; Highly Expressed In Cancer,Rich In Leucine Heptad Repeats

Kinetochore Associated Protein 2 (KNTC2)
CCC H754
HGNC 16909
MGI 1914302
PubMed 19878654
RGD 1310154
UniProt O14777
HEC is one of several proteins involved in spindle checkpoint signaling. This surveillance mechanism assures correct segregation of chromosomes during cell division by detecting unaligned chromosomes and causing prometaphase arrest until the proper bipolar attachment of chromosomes is achieved.
The deduced 642-amino acid protein has a calculated molecular mass of 72 kD. HEC has a series of typical leucine heptad repeats that covers the last two-thirds of its sequence. Northern blot analysis revealed a 2.3-kb mRNA in all cell lines tested. Tumor cell lines expressed HEC more abundantly than did normal tissues and untransformed cells. Northern blot analysis of synchronized CV1 monkey kidney cells showed highest expression during S and M phases.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Kinetochore Associated Protein 2 (KNTC2) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Kinetochore Associated Protein 2 (KNTC2) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Kinetochore Associated Protein 2 (KNTC2) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Kinetochore Associated Protein 2 (KNTC2) CLIA Kit Customized Service Offer
n/a ELISA Kit for Kinetochore Associated Protein 2 (KNTC2) ELISA Kit Customized Service Offer

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Kinetochore Associated Protein 2 (KNTC2) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Kinetochore Associated Protein 2 (KNTC2) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Kinetochore Associated Protein 2 (KNTC2) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Kinetochore Associated Protein 2 (KNTC2) CLIA Kit Customized Service Offer
n/a ELISA Kit for Kinetochore Associated Protein 2 (KNTC2) ELISA Kit Customized Service Offer

Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Kinetochore Associated Protein 2 (KNTC2) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Kinetochore Associated Protein 2 (KNTC2) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Kinetochore Associated Protein 2 (KNTC2) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Kinetochore Associated Protein 2 (KNTC2) CLIA Kit Customized Service Offer
n/a ELISA Kit for Kinetochore Associated Protein 2 (KNTC2) ELISA Kit Customized Service Offer
  1. "HEC, a novel nuclear protein rich in leucine heptad repeats specifically involved in mitosis."Mol. Cell. Biol. 17:6049-6056(1997) [PubMed] [Europe PMC] [Abstract]
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
  3. "HEC binds to the seventh regulatory subunit of the 26 S proteasome and modulates the proteolysis of mitotic cyclins."J. Biol. Chem. 272:24081-24087(1997) [PubMed] [Europe PMC] [Abstract]
  4. "Hec1p, an evolutionarily conserved coiled-coil protein, modulates chromosome segregation through interaction with SMC proteins."Mol. Cell. Biol. 19:5417-5428(1999) [PubMed] [Europe PMC] [Abstract]
  5. "Retinoblastoma protein enhances the fidelity of chromosome segregation mediated by hsHec1p."Mol. Cell. Biol. 20:3529-3537(2000) [PubMed] [Europe PMC] [Abstract]
  6. "Phosphorylation of the mitotic regulator protein Hec1 by Nek2 kinase is essential for faithful chromosome segregation."J. Biol. Chem. 277:49408-49416(2002) [PubMed] [Europe PMC] [Abstract]
  7. "Role of Hec1 in spindle checkpoint signaling and kinetochore recruitment of Mad1/Mad2."Science 297:2267-2270(2002) [PubMed] [Europe PMC] [Abstract]
  8. "Nuf2 and Hec1 are required for retention of the checkpoint proteins Mad1 and Mad2 to kinetochores."Curr. Biol. 13:2103-2109(2003) [PubMed] [Europe PMC] [Abstract]
  9. "Kinetochore localization and microtubule interaction of the human spindle checkpoint kinase Mps1."Chromosoma 113:1-15(2004) [PubMed] [Europe PMC] [Abstract]
  10. "The RanGAP1-RanBP2 complex is essential for microtubule-kinetochore interactions in vivo."Curr. Biol. 14:611-617(2004) [PubMed] [Europe PMC] [Abstract]
  11. "Timing and checkpoints in the regulation of mitotic progression."Dev. Cell 7:45-60(2004) [PubMed] [Europe PMC] [Abstract]
  12. "Sgt1 is required for human kinetochore assembly."EMBO Rep. 5:626-631(2004) [PubMed] [Europe PMC] [Abstract]
  13. "Identification of two novel components of the human NDC80 kinetochore complex."J. Biol. Chem. 279:13076-13085(2004) [PubMed] [Europe PMC] [Abstract]
  14. "NEK2A interacts with MAD1 and possibly functions as a novel integrator of the spindle checkpoint signaling."J. Biol. Chem. 279:20049-20057(2004) [PubMed] [Europe PMC] [Abstract]
  15. "Identification of the substrates and interaction proteins of aurora kinases from a protein-protein interaction model."Mol. Cell. Proteomics 3:93-104(2004) [PubMed] [Europe PMC] [Abstract]
  16. "A conserved Mis12 centromere complex is linked to heterochromatic HP1 and outer kinetochore protein Zwint-1."Nat. Cell Biol. 6:1135-1141(2004) [PubMed] [Europe PMC] [Abstract]
  17. "Polo-like kinase 1 creates the tension-sensing 3F3/2 phosphoepitope and modulates the association of spindle-checkpoint proteins at kinetochores."Curr. Biol. 15:1078-1089(2005) [PubMed] [Europe PMC] [Abstract]
  18. "Architecture of the human Ndc80-Hec1 complex, a critical constituent of the outer kinetochore."J. Biol. Chem. 280:29088-29095(2005) [PubMed] [Europe PMC] [Abstract]
  19. "ZW10 links mitotic checkpoint signaling to the structural kinetochore."J. Cell Biol. 169:49-60(2005) [PubMed] [Europe PMC] [Abstract]
  20. "Hec1 and Nuf2 are core components of the kinetochore outer plate essential for organizing microtubule attachment sites."Mol. Biol. Cell 16:519-531(2005) [PubMed] [Europe PMC] [Abstract]
  21. "The functional region of CENP-H interacts with the Nuf2 complex that localizes to centromere during mitosis."Mol. Cell. Biol. 25:1958-1970(2005) [PubMed] [Europe PMC] [Abstract]
  22. "Hec1 sequentially recruits Zwint-1 and ZW10 to kinetochores for faithful chromosome segregation and spindle checkpoint control."Oncogene 25:6901-6914(2006) [PubMed] [Europe PMC] [Abstract]
  23. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
  24. "Initial characterization of the human central proteome."BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]