≡

Hexamethylene Bis Acetamide Inducible Protein 1 (HEXIM1)

[Edit]

EDG1; HIS1; CLP1; MAQ1; Cardiac lineage protein 1; Estrogen down-regulated gene 1 protein; Menage a quatre protein 1

Hexamethylene Bis Acetamide Inducible Protein 1 (HEXIM1)
CCC H915
HGNC 24953
MGI 2385923
PubMed 24515107
RGD 1559851
UniProt O94992
Wiki HEXIM1
HEXIM1 inhibited the ability of C2TA (MHC2TA) to activate transcription of MHC class II genes in HeLa cells. This inhibition depended on the cyclin T1 (CCNT1)-binding domain of HEXIM1. In vitro binding experiments and chromatin immunoprecipitation analyses showed that HEXIM1 competed with C2TA for binding to CCNT1, which together with CDK9 forms the positive transcription elongation factor B (PTEFB), and sequestered PTEFB from C2TA. HeLa cells depleted of HEXIM1 by small interfering RNA displayed increased C2TA activity and induction of C2TA-dependent genes.The deduced 41-kD protein is 85.3% homologous to human HIS1. Northern and Western blot analyses revealed wide expression during postnatal development, with highest levels in heart, skeletal muscle, and brain.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Hexamethylene Bis Acetamide Inducible Protein 1 (HEXIM1) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Hexamethylene Bis Acetamide Inducible Protein 1 (HEXIM1) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Hexamethylene Bis Acetamide Inducible Protein 1 (HEXIM1) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Hexamethylene Bis Acetamide Inducible Protein 1 (HEXIM1) CLIA Kit Customized Service Offer
n/a ELISA Kit for Hexamethylene Bis Acetamide Inducible Protein 1 (HEXIM1) ELISA Kit Customized Service Offer

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Hexamethylene Bis Acetamide Inducible Protein 1 (HEXIM1) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Hexamethylene Bis Acetamide Inducible Protein 1 (HEXIM1) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Hexamethylene Bis Acetamide Inducible Protein 1 (HEXIM1) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Hexamethylene Bis Acetamide Inducible Protein 1 (HEXIM1) CLIA Kit Customized Service Offer
n/a ELISA Kit for Hexamethylene Bis Acetamide Inducible Protein 1 (HEXIM1) ELISA Kit Customized Service Offer

Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Hexamethylene Bis Acetamide Inducible Protein 1 (HEXIM1) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Hexamethylene Bis Acetamide Inducible Protein 1 (HEXIM1) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Hexamethylene Bis Acetamide Inducible Protein 1 (HEXIM1) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Hexamethylene Bis Acetamide Inducible Protein 1 (HEXIM1) CLIA Kit Customized Service Offer
n/a ELISA Kit for Hexamethylene Bis Acetamide Inducible Protein 1 (HEXIM1) ELISA Kit Customized Service Offer
  1. "Cloning of hexamethylene-bis-acetamide-inducible transcript, HEXIM1, in human vascular smooth muscle cells."Biomed. Res. 20:273-279(1999)
  2. "Complete sequencing and characterization of 21,243 full-length human cDNAs." Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
  4. "Identification of a novel inhibitor of breast cell growth that is down-regulated by estrogens and decreased in breast tumors."Cancer Res. 63:5151-5158(2003) [PubMed] [Europe PMC] [Abstract]
  5. "Suppression of NF-kappaB-dependent gene expression by a hexamethylene bisacetamide-inducible protein HEXIM1 in human vascular smooth muscle cells."Genes Cells 8:95-107(2003) [PubMed] [Europe PMC] [Abstract]
  6. "Inhibition of P-TEFb (CDK9/Cyclin T) kinase and RNA polymerase II transcription by the coordinated actions of HEXIM1 and 7SK snRNA."Mol. Cell 12:971-982(2003) [PubMed] [Europe PMC] [Abstract]
  7. "MAQ1 and 7SK RNA interact with CDK9/cyclin T complexes in a transcription-dependent manner."Mol. Cell. Biol. 23:4859-4869(2003) [PubMed] [Europe PMC] [Abstract]
  8. "Binding of the 7SK snRNA turns the HEXIM1 protein into a P-TEFb (CDK9/cyclin T) inhibitor."EMBO J. 23:2608-2619(2004) [PubMed] [Europe PMC] [Abstract]
  9. "A human immunodeficiency virus type 1 Tat-like arginine-rich RNA-binding domain is essential for HEXIM1 to inhibit RNA polymerase II transcription through 7SK snRNA-mediated inactivation of P-TEFb."Mol. Cell. Biol. 24:5094-5105(2004) [PubMed] [Europe PMC] [Abstract]
  10. "Interplay between 7SK snRNA and oppositely charged regions in HEXIM1 direct the inhibition of P-TEFb."EMBO J. 24:4291-4303(2005) [PubMed] [Europe PMC] [Abstract]
  11. "Compensatory contributions of HEXIM1 and HEXIM2 in maintaining the balance of active and inactive positive transcription elongation factor b complexes for control of transcription."J. Biol. Chem. 280:16368-16376(2005) [PubMed] [Europe PMC] [Abstract]
  12. "Identification of a cyclin T-binding domain in Hexim1 and biochemical analysis of its binding competition with HIV-1 Tat."J. Biol. Chem. 280:24968-24977(2005) [PubMed] [Europe PMC] [Abstract]
  13. "Analysis of the large inactive P-TEFb complex indicates that it contains one 7SK molecule, a dimer of HEXIM1 or HEXIM2, and two P-TEFb molecules containing Cdk9 phosphorylated at threonine 186."J. Biol. Chem. 280:28819-28826(2005) [PubMed] [Europe PMC] [Abstract]
  14. "Oligomerization of HEXIM1 via 7SK snRNA and coiled-coil region directs the inhibition of P-TEFb."Nucleic Acids Res. 33:7000-7010(2005) [PubMed] [Europe PMC] [Abstract]
  15. "The breast cell growth inhibitor, estrogen down regulated gene 1, modulates a novel functional interaction between estrogen receptor alpha and transcriptional elongation factor cyclin T1."Oncogene 24:5576-5588(2005) [PubMed] [Europe PMC] [Abstract]
  16. "HEXIM1 forms a transcriptionally abortive complex with glucocorticoid receptor without involving 7SK RNA and positive transcription elongation factor b."Proc. Natl. Acad. Sci. U.S.A. 102:8555-8560(2005) [PubMed] [Europe PMC] [Abstract]
  17. "Hexim1 sequesters positive transcription elongation factor b from the class II transactivator on MHC class II promoters."Proc. Natl. Acad. Sci. U.S.A. 103:17349-17354(2006) [PubMed] [Europe PMC] [Abstract]
  18. "Systematic analysis of the protein interaction network for the human transcription machinery reveals the identity of the 7SK capping enzyme."Mol. Cell 27:262-274(2007) [PubMed] [Europe PMC] [Abstract]
  19. "Regulation of P-TEFb elongation complex activity by CDK9 acetylation."Mol. Cell. Biol. 27:4641-4651(2007) [PubMed] [Europe PMC] [Abstract]
  20. "HEXIM1 is a promiscuous double-stranded RNA-binding protein and interacts with RNAs in addition to 7SK in cultured cells."Nucleic Acids Res. 35:2503-2512(2007) [PubMed] [Europe PMC] [Abstract]
  21. "A quantitative atlas of mitotic phosphorylation."Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
  22. "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
  23. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
  24. "Initial characterization of the human central proteome."BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
  25. "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
  26. "Structure of the Cyclin T binding domain of Hexim1 and molecular basis for its recognition of P-TEFb."Proc. Natl. Acad. Sci. U.S.A. 104:14312-14317(2007) [PubMed] [Europe PMC] [Abstract]