GRB2 Related Adaptor Protein 2 (GRAP2)
[Edit]P38; GADS; GRAP-2; GRB2L; GRBLG; GRID; GRPL; GrbX; Grf40; Mona; Growth factor receptor-binding protein; Hematopoietic cell-associated adapter protein GrpL
The predicted 330-amino acid GRAP2 protein contains an SH2 domain flanked by 2 SH3 domains. These domains are arranged in the same configuration as, and share a high degree of amino acid identity with, the SH2 and SH3 domains of the GRB2 and GRAP proteins. However, GRAP2 contains an additional 120-amino acid glutamine-rich domain between its SH2 domain and C-terminal SH3 domain.
The interaction of GRAP2 with GAB1 appears to be mediated through its C-terminal SH3 domain. Northern blot analysis detected major 1.4- and 4.0-kb GRAP2 transcripts in thymus, spleen, peripheral blood leukocytes, and chronic myelogenous and lymphoblastic leukemia cell lines, with the highest expression in thymus.
Organism species: Homo sapiens (Human)
CATALOG NO. | PRODUCT NAME | APPLICATIONS | |
Proteins | RPH967Hu01 | Recombinant GRB2 Related Adaptor Protein 2 (GRAP2) | Positive Control; Immunogen; SDS-PAGE; WB. |
Antibodies | PAH967Hu01 | Polyclonal Antibody to GRB2 Related Adaptor Protein 2 (GRAP2) | WB; IHC; ICC; IP. |
Assay Kits | SEH967Hu | ELISA Kit for GRB2 Related Adaptor Protein 2 (GRAP2) | Enzyme-linked immunosorbent assay for Antigen Detection. |
Organism species: Mus musculus (Mouse)
CATALOG NO. | PRODUCT NAME | APPLICATIONS | |
Proteins | RPH967Mu01 | Recombinant GRB2 Related Adaptor Protein 2 (GRAP2) | Positive Control; Immunogen; SDS-PAGE; WB. |
Antibodies | PAH967Mu01 | Polyclonal Antibody to GRB2 Related Adaptor Protein 2 (GRAP2) | WB; IHC; ICC; IP. |
Assay Kits | n/a | CLIA Kit for GRB2 Related Adaptor Protein 2 (GRAP2) | CLIA Kit Customized Service Offer |
n/a | ELISA Kit for GRB2 Related Adaptor Protein 2 (GRAP2) | ELISA Kit Customized Service Offer |
Organism species: Rattus norvegicus (Rat)
CATALOG NO. | PRODUCT NAME | APPLICATIONS | |
Proteins | RPH967Ra01 | Recombinant GRB2 Related Adaptor Protein 2 (GRAP2) | Positive Control; Immunogen; SDS-PAGE; WB. |
Antibodies | PAH967Ra01 | Polyclonal Antibody to GRB2 Related Adaptor Protein 2 (GRAP2) | WB; IHC; ICC; IP. |
Assay Kits | n/a | CLIA Kit for GRB2 Related Adaptor Protein 2 (GRAP2) | CLIA Kit Customized Service Offer |
n/a | ELISA Kit for GRB2 Related Adaptor Protein 2 (GRAP2) | ELISA Kit Customized Service Offer |
- "Molecular cloning and expression of human grap-2, a novel leukocyte-specific SH2- and SH3-containing adaptor-like protein that binds to gab-1."Biochem. Biophys. Res. Commun. 253:443-447(1998) [PubMed] [Europe PMC] [Abstract]
- "GrpL, a Grb2-related adaptor protein, interacts with SLP-76 to regulate nuclear factor of activated T cell activation."J. Exp. Med. 189:1243-1253(1999) [PubMed] [Europe PMC] [Abstract]
- "Grf40, A novel Grb2 family member, is involved in T cell signaling through interaction with SLP-76 and LAT."J. Exp. Med. 189:1383-1390(1999) [PubMed] [Europe PMC] [Abstract]
- "GRID: a novel Grb-2-related adapter protein that interacts with the activated T cell costimulatory receptor CD28."J. Immunol. 164:5805-5814(2000) [PubMed] [Europe PMC] [Abstract]
- "Genomic organization and restricted expression of the human Mona/Gads gene suggests regulation by two specific promoters."Gene 290:173-179(2002) [PubMed] [Europe PMC] [Abstract]
- "A genome annotation-driven approach to cloning the human ORFeome."Genome Biol. 5:R84.1-R84.11(2004) [PubMed] [Europe PMC] [Abstract]
- "Complete sequencing and characterization of 21,243 full-length human cDNAs." Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
- "The DNA sequence of human chromosome 22." Nature 402:489-495(1999) [PubMed] [Europe PMC] [Abstract]
- "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
- "Association of Grb2, Gads, and phospholipase C-gamma 1 with phosphorylated LAT tyrosine residues. Effect of LAT tyrosine mutations on T cell antigen receptor-mediated signaling."J. Biol. Chem. 275:23355-23361(2000) [PubMed] [Europe PMC] [Abstract]
- "Shb links SLP-76 and Vav with the CD3 complex in Jurkat T cells."Eur. J. Biochem. 269:3279-3288(2002) [PubMed] [Europe PMC] [Abstract]
- "Molecular cloning of a novel gene encoding a membrane-associated adaptor protein (LAX) in lymphocyte signaling."J. Biol. Chem. 277:46151-46158(2002) [PubMed] [Europe PMC] [Abstract]
- "Profiling of tyrosine phosphorylation pathways in human cells using mass spectrometry."Proc. Natl. Acad. Sci. U.S.A. 100:443-448(2003) [PubMed] [Europe PMC] [Abstract]
- "Robust phosphoproteomic profiling of tyrosine phosphorylation sites from human T cells using immobilized metal affinity chromatography and tandem mass spectrometry."Anal. Chem. 76:2763-2772(2004) [PubMed] [Europe PMC] [Abstract]
- "Phosphoproteome of resting human platelets."J. Proteome Res. 7:526-534(2008) [PubMed] [Europe PMC] [Abstract]
- "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
- "Lysine acetylation targets protein complexes and co-regulates major cellular functions."Science 325:834-840(2009) [PubMed] [Europe PMC] [Abstract]
- "Histidine domain-protein tyrosine phosphatase interacts with Grb2 and GrpL."PLoS ONE 5:E14339-E14339(2010) [PubMed] [Europe PMC] [Abstract]
- "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]