Golgin B1 (GOLGB1)
[Edit]GCP; GCP372; GOLIM1; GIANTIN; Golgi Autoantigen,Golgin Subfamily b; Macrogolgin 1; Golgi Integral Membrane Protein; 372 kDa Golgi complex-associated Protein
The antibody stained Golgi specifically in several human tissues, as well as in rat, hamster, mouse, and chicken cells. On Western blots, the antibody recognized a 400-kD protein, dubbed 'giantin' because of its large size. Giantin is not extracted from the membrane by high salt, urea, alkaline carbonate, or Triton X-100 (nonionic detergent) treatment, suggesting that it is an integral component of the Golgi membrane that is associated with the cytoskeleton. Protease digestion experiments suggest that giantin has a large (at least 350 kD) cytoplasmic domain. The predicted pI is 4.77, and the protein contains a high fraction of alpha-helical domains with heptad repeats that can form coiled-coils. On Western blots, the antibodies recognize 320- and 380-kD proteins. Anti-macrogolgin antibodies were found in 11% of HIV-positive patients.
Organism species: Homo sapiens (Human)
CATALOG NO. | PRODUCT NAME | APPLICATIONS | |
Proteins | n/a | Recombinant Golgin B1 (GOLGB1) | Recombinant Protein Customized Service Offer |
Antibodies | n/a | Monoclonal Antibody to Golgin B1 (GOLGB1) | Monoclonal Antibody Customized Service Offer |
n/a | Polyclonal Antibody to Golgin B1 (GOLGB1) | Polyclonal Antibody Customized Service Offer | |
Assay Kits | n/a | CLIA Kit for Golgin B1 (GOLGB1) | CLIA Kit Customized Service Offer |
n/a | ELISA Kit for Golgin B1 (GOLGB1) | ELISA Kit Customized Service Offer |
Organism species: Mus musculus (Mouse)
CATALOG NO. | PRODUCT NAME | APPLICATIONS | |
Proteins | n/a | Recombinant Golgin B1 (GOLGB1) | Recombinant Protein Customized Service Offer |
Antibodies | n/a | Monoclonal Antibody to Golgin B1 (GOLGB1) | Monoclonal Antibody Customized Service Offer |
n/a | Polyclonal Antibody to Golgin B1 (GOLGB1) | Polyclonal Antibody Customized Service Offer | |
Assay Kits | n/a | CLIA Kit for Golgin B1 (GOLGB1) | CLIA Kit Customized Service Offer |
n/a | ELISA Kit for Golgin B1 (GOLGB1) | ELISA Kit Customized Service Offer |
Organism species: Rattus norvegicus (Rat)
CATALOG NO. | PRODUCT NAME | APPLICATIONS | |
Proteins | n/a | Recombinant Golgin B1 (GOLGB1) | Recombinant Protein Customized Service Offer |
Antibodies | n/a | Monoclonal Antibody to Golgin B1 (GOLGB1) | Monoclonal Antibody Customized Service Offer |
n/a | Polyclonal Antibody to Golgin B1 (GOLGB1) | Polyclonal Antibody Customized Service Offer | |
Assay Kits | n/a | CLIA Kit for Golgin B1 (GOLGB1) | CLIA Kit Customized Service Offer |
n/a | ELISA Kit for Golgin B1 (GOLGB1) | ELISA Kit Customized Service Offer |
- "Molecular genetic analyses of a 376-kilodalton Golgi complex membrane protein (giantin)."Mol. Cell. Biol. 14:2564-2576(1994) [PubMed] [Europe PMC] [Abstract]
- "Macrogolgin -- a new 376 kD Golgi complex outer membrane protein as target of antibodies in patients with rheumatic diseases and HIV infections."J. Autoimmun. 7:67-91(1994) [PubMed] [Europe PMC] [Abstract]
- "Molecular cloning and sequence analysis of a human 372-kDa protein localized in the Golgi complex."Biochem. Biophys. Res. Commun. 205:1399-1408(1994) [PubMed] [Europe PMC] [Abstract]
- "Fine expression profiling of full-length transcripts using a size-unbiased cDNA library prepared with the vector-capping method."DNA Res. 15:123-136(2008) [PubMed] [Europe PMC] [Abstract]
- "Full-length transcriptome analysis of human retina-derived cell lines ARPE-19 and Y79 using the vector-capping method."Invest. Ophthalmol. Vis. Sci. 52:6662-6670(2011) [PubMed] [Europe PMC] [Abstract]
- "The DNA sequence, annotation and analysis of human chromosome 3." Nature 440:1194-1198(2006) [PubMed] [Europe PMC] [Abstract]
- "Polo-like kinase 3 is Golgi localized and involved in regulating Golgi fragmentation during the cell cycle."Exp. Cell Res. 294:51-59(2004) [PubMed] [Europe PMC] [Abstract]
- "A quantitative atlas of mitotic phosphorylation."Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
- "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
- "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
- "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
- "Initial characterization of the human central proteome."BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
- "N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]
- "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]
- "The consensus coding sequences of human breast and colorectal cancers." Science 314:268-274(2006) [PubMed] [Europe PMC] [Abstract]