Epsin 3 (EPN3)

The deduced 632-amino acid protein has a calculated molecular mass of 68 kD. EPN3 has the same tripartite domain structure as EPN1 and EPN2, including an epsin N-terminal homology (ENTH) domain, multiple DPW sequences and a clathrin-binding motif in its central region, and a C-terminal consensus sequence of 3 NPF repeats that bind EPS15. The ENTH domain of EPN3 shares 80% and 82% sequence identity with those of EPN1 and EPN2, respectively, and the 3 NPF repeats are 100% conserved in these 3 proteins. EPN3 has a distal low-affinity clathrin-binding sequence in addition to the high-affinity site. EPN3 was absent from freshly isolated intact skin and was not expressed in most adult and fetal tissues examined. Expression was detected in stomach only after extended exposure.