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Development And Differentiation Enhancing Factor 1 (DDEF1)

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DEF1; ASAP1; PAP; AMAP1; PAG2; CENTB4; Centaurin,Beta 4; ArfGAP With SH3 Domain,Ankyrin Repeat And PH Domain 1; ADP-ribosylation factor-directed GTPase-activating 1

Development And Differentiation Enhancing Factor 1 (DDEF1)
CCC J284
HGNC 2720
MGI 1342335
PubMed 18843202
RGD 1307379
UniProt Q9ULH1
Wiki DDEF1
Membrane trafficking is regulated in part by small GTP-binding proteins of the ADP-ribosylation factor (ARF) family . ARF function depends on the controlled exchange and hydrolysis of GTP. DDEF1 sequence contains a pleckstrin homology (PH) domain, a zinc finger motif, 3 ankyrin repeats, a proline-rich region containing several SH3 ligand motifs, and an SH3 domain. Northern blot analysis of mouse tissues revealed expression in a variety of tissues. Western blot analysis detected crossreacting proteins, suggesting that ASAP1 is part of a gene family. Studies of the expressed ASAP1 protein showed that it has GAP activity on ARF1 and ARF5 substrates. ASAP1 binds to SH3 domains, including those of SRC and CRK. Both isoforms of ASAP1 became tyrosine phosphorylated by active SRC.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Development And Differentiation Enhancing Factor 1 (DDEF1) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Development And Differentiation Enhancing Factor 1 (DDEF1) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Development And Differentiation Enhancing Factor 1 (DDEF1) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Development And Differentiation Enhancing Factor 1 (DDEF1) CLIA Kit Customized Service Offer
n/a ELISA Kit for Development And Differentiation Enhancing Factor 1 (DDEF1) ELISA Kit Customized Service Offer

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Development And Differentiation Enhancing Factor 1 (DDEF1) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Development And Differentiation Enhancing Factor 1 (DDEF1) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Development And Differentiation Enhancing Factor 1 (DDEF1) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Development And Differentiation Enhancing Factor 1 (DDEF1) CLIA Kit Customized Service Offer
n/a ELISA Kit for Development And Differentiation Enhancing Factor 1 (DDEF1) ELISA Kit Customized Service Offer

Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Development And Differentiation Enhancing Factor 1 (DDEF1) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Development And Differentiation Enhancing Factor 1 (DDEF1) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Development And Differentiation Enhancing Factor 1 (DDEF1) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Development And Differentiation Enhancing Factor 1 (DDEF1) CLIA Kit Customized Service Offer
n/a ELISA Kit for Development And Differentiation Enhancing Factor 1 (DDEF1) ELISA Kit Customized Service Offer
  1. "DNA sequence and analysis of human chromosome 8." Nature 439:331-335(2006) [PubMed] [Europe PMC] [Abstract]
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
  3. "The full-ORF clone resource of the German cDNA consortium."BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract]
  4. "Prediction of the coding sequences of unidentified human genes. XV. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro."DNA Res. 6:337-345(1999) [PubMed] [Europe PMC] [Abstract]
  5. "ASAP1, a phospholipid-dependent arf GTPase-activating protein that associates with and is phosphorylated by Src."Mol. Cell. Biol. 18:7038-7051(1998) [PubMed] [Europe PMC] [Abstract]
  6. "Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis."J. Proteome Res. 7:1346-1351(2008) [PubMed] [Europe PMC] [Abstract]
  7. "Phosphoproteome of resting human platelets."J. Proteome Res. 7:526-534(2008) [PubMed] [Europe PMC] [Abstract]
  8. "A quantitative atlas of mitotic phosphorylation."Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
  9. "Functional genomic screen for modulators of ciliogenesis and cilium length."Nature 464:1048-1051(2010) [PubMed] [Europe PMC] [Abstract]
  10. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
  11. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]
  12. "Targeting AMAP1 and cortactin binding bearing an atypical src homology 3/proline interface for prevention of breast cancer invasion and metastasis."Proc. Natl. Acad. Sci. U.S.A. 103:7036-7041(2006) [PubMed] [Europe PMC] [Abstract]
  13. "Solution structure of the PH domain of PIP2-dependent ARF1 GTPase-activating protein from human."Submitted (JUN-2006) to the PDB data bank
  14. "Solution structure of the SH3 domain of 130 kDA phosphatidylinositol 4,5-biphosphate-dependent ARF1 GTPase-activating protein."Submitted (FEB-2009) to the PDB data bank
  15. "Structural basis of the recognition of the SAMP motif of adenomatous polyposis coli by the Src-homology 3 domain."Biochemistry 49:5143-5153(2010) [PubMed] [Europe PMC] [Abstract]