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Deoxynucleotidyltransferase, Terminal (DNTT)

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TDT; Terminal Deoxynucleotidyl Transferase; DNA Nucleotidylexotransferase; Terminal Transferase; Terminal addition enzyme; DNA nucleotidylexotransferase

Deoxynucleotidyltransferase, Terminal (DNTT)
CCC J425
HGNC 2983
MGI 98659
PubMed 3862101
RGD 1305054
UniProt P04053
Wiki DNTT
Terminal deoxynucleotidyl transferase is a specialized DNA polymerase expressed in immature, pre-B, pre-T lymphoid cells, and acute lymphoblastic leukemia/lymphoma cells. TdT adds N-nucleotides to the variable, diversity, and joining exons during antibody gene recombination.
TdT catalyses the addition of nucleotides to the 3' terminus of a DNA molecule. Unlike most DNA polymerases it does not require a template. The preferred substrate of this enzyme is a 3'-overhang, but it can also add nucleotides to blunt or recessed 3' ends. Cobalt is a necessary cofactor, however the enzyme catalyzes reaction upon Mg and Mn administration in vitro.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Deoxynucleotidyltransferase, Terminal (DNTT) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Deoxynucleotidyltransferase, Terminal (DNTT) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Deoxynucleotidyltransferase, Terminal (DNTT) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Deoxynucleotidyltransferase, Terminal (DNTT) CLIA Kit Customized Service Offer
n/a ELISA Kit for Deoxynucleotidyltransferase, Terminal (DNTT) ELISA Kit Customized Service Offer

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Deoxynucleotidyltransferase, Terminal (DNTT) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Deoxynucleotidyltransferase, Terminal (DNTT) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Deoxynucleotidyltransferase, Terminal (DNTT) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Deoxynucleotidyltransferase, Terminal (DNTT) CLIA Kit Customized Service Offer
n/a ELISA Kit for Deoxynucleotidyltransferase, Terminal (DNTT) ELISA Kit Customized Service Offer

Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Deoxynucleotidyltransferase, Terminal (DNTT) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Deoxynucleotidyltransferase, Terminal (DNTT) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Deoxynucleotidyltransferase, Terminal (DNTT) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Deoxynucleotidyltransferase, Terminal (DNTT) CLIA Kit Customized Service Offer
n/a ELISA Kit for Deoxynucleotidyltransferase, Terminal (DNTT) ELISA Kit Customized Service Offer

Organism species: Bos taurus; Bovine (Cattle)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPJ425Bo01 Recombinant Deoxynucleotidyltransferase, Terminal (DNTT) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies n/a Monoclonal Antibody to Deoxynucleotidyltransferase, Terminal (DNTT) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Deoxynucleotidyltransferase, Terminal (DNTT) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Deoxynucleotidyltransferase, Terminal (DNTT) CLIA Kit Customized Service Offer
n/a ELISA Kit for Deoxynucleotidyltransferase, Terminal (DNTT) ELISA Kit Customized Service Offer
  1. "Expression of human terminal deoxynucleotidyl transferase in Escherichia coli."J. Biol. Chem. 260:10495-10502(1985) [PubMed] [Europe PMC] [Abstract]
  2. "Human terminal deoxyribonucleotidyltransferase: molecular cloning and structural analysis of the gene and 5' flanking region."Proc. Natl. Acad. Sci. U.S.A. 85:2489-2493(1988) [PubMed] [Europe PMC] [Abstract]
  3. "Terminal deoxynucleotidyltransferase is negatively regulated by direct interaction with proliferating cell nuclear antigen."Genes Cells 6:815-824(2001) [PubMed] [Europe PMC] [Abstract]
  4. "The DNA sequence and comparative analysis of human chromosome 10." Nature 429:375-381(2004) [PubMed] [Europe PMC] [Abstract]
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
  6. "Isolation of putative promoter region for human terminal deoxynucleotidyltransferase gene."Biochem. Biophys. Res. Commun. 154:91-100(1988) [PubMed] [Europe PMC] [Abstract]
  7. "Molecular cloning of human terminal deoxynucleotidyltransferase."Proc. Natl. Acad. Sci. U.S.A. 81:4363-4367(1984) [PubMed] [Europe PMC] [Abstract]
  8. "Mutational analysis of residues in the nucleotide binding domain of human terminal deoxynucleotidyl transferase."J. Biol. Chem. 269:11859-11868(1994) [PubMed] [Europe PMC] [Abstract]
  9. "Terminal deoxynucleotidyltransferase directly interacts with a novel nuclear protein that is homologous to p65."Genes Cells 6:641-652(2001) [PubMed] [Europe PMC] [Abstract]
  10. "Terminal deoxynucleotidyltransferase forms a ternary complex with a novel chromatin remodeling protein with 82 kDa and core histone."Genes Cells 8:559-571(2003) [PubMed] [Europe PMC] [Abstract]
  11. "Role of human Pso4 in mammalian DNA repair and association with terminal deoxynucleotidyl transferase."Proc. Natl. Acad. Sci. U.S.A. 100:10746-10751(2003) [PubMed] [Europe PMC] [Abstract]
  12. "Direct binding of TReP-132 with TdT results in reduction of TdT activity."Genes Cells 11:47-57(2006) [PubMed] [Europe PMC] [Abstract]
  13. "Solution structure of BRCT domain of terminal deoxynucleotidyltransferase."Submitted (NOV-2005) to the PDB data bank