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Claspin (CLSPN)

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Claspin Homolog

Claspin (CLSPN)
CCC J441
HGNC 19715
MGI 2445153
RGD 1309083
UniProt Q9HAW4
Claspin localized in nuclei and associated with CHK1 following replication stress or other types of DNA damage. Claspin was phosphorylated in response to replication stress, and phosphorylation appeared to be required for the association between claspin and CHK1. In addition, claspin interacted with the checkpoint proteins ATR and RAD9. Since ATR and the RAD9-RAD1-HUS1 complex are involved in CHK1 activation, the authors hypothesized that claspin may be an adaptor that brings these molecules together. Downregulation of claspin by small interfering RNA inhibited CHK1 activation in response to replication stress, augmented the premature chromatin condensation induced by hydroxyurea, inhibited ultraviolet-induced reduction of DNA synthesis, and decreased cell survival.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Claspin (CLSPN) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Claspin (CLSPN) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Claspin (CLSPN) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Claspin (CLSPN) CLIA Kit Customized Service Offer
n/a ELISA Kit for Claspin (CLSPN) ELISA Kit Customized Service Offer

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Claspin (CLSPN) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Claspin (CLSPN) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Claspin (CLSPN) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Claspin (CLSPN) CLIA Kit Customized Service Offer
n/a ELISA Kit for Claspin (CLSPN) ELISA Kit Customized Service Offer

Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Claspin (CLSPN) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Claspin (CLSPN) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Claspin (CLSPN) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Claspin (CLSPN) CLIA Kit Customized Service Offer
n/a ELISA Kit for Claspin (CLSPN) ELISA Kit Customized Service Offer
  1. "Claspin, a novel protein required for the activation of Chk1 during a DNA replication checkpoint response in Xenopus egg extracts."Mol. Cell 6:839-849(2000) [PubMed] [Europe PMC] [Abstract]
  2. "The DNA sequence and biological annotation of human chromosome 1." Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract]
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
  4. "Human claspin is required for replication checkpoint control."J. Biol. Chem. 278:30057-30062(2003) [PubMed] [Europe PMC] [Abstract]
  5. "ATR, claspin and the Rad9-Rad1-Hus1 complex regulate Chk1 and Cdc25A in the absence of DNA damage."Cell Cycle 3:941-945(2004) [PubMed] [Europe PMC] [Abstract]
  6. "Human claspin is a ring-shaped DNA-binding protein with high affinity to branched DNA structures."J. Biol. Chem. 279:39289-39295(2004) [PubMed] [Europe PMC] [Abstract]
  7. "Human claspin works with BRCA1 to both positively and negatively regulate cell proliferation."Proc. Natl. Acad. Sci. U.S.A. 101:6484-6489(2004) [PubMed] [Europe PMC] [Abstract]
  8. "DNA-dependent phosphorylation of Chk1 and claspin in a human cell-free system."Biochem. J. 388:705-712(2005) [PubMed] [Europe PMC] [Abstract]
  9. "A role for the deubiquitinating enzyme USP28 in control of the DNA-damage response."Cell 126:529-542(2006) [PubMed] [Europe PMC] [Abstract]
  10. "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract]
  11. "Repeated phosphopeptide motifs in human Claspin are phosphorylated by Chk1 and mediate Claspin function."J. Biol. Chem. 281:33276-33282(2006) [PubMed] [Europe PMC] [Abstract]
  12. "Mammalian TIMELESS and Tipin are evolutionarily conserved replication fork-associated factors."J. Mol. Biol. 366:36-52(2007) [PubMed] [Europe PMC] [Abstract]
  13. "ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage."Science 316:1160-1166(2007) [PubMed] [Europe PMC] [Abstract]
  14. "The Cdc14B-Cdh1-Plk1 axis controls the G2 DNA-damage-response checkpoint."Cell 134:256-267(2008) [PubMed] [Europe PMC] [Abstract]
  15. "A quantitative atlas of mitotic phosphorylation."Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
  16. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
  17. "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
  18. "Lysine acetylation targets protein complexes and co-regulates major cellular functions."Science 325:834-840(2009) [PubMed] [Europe PMC] [Abstract]
  19. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
  20. "Initial characterization of the human central proteome."BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
  21. "Role for casein kinase 1 in the phosphorylation of Claspin on critical residues necessary for the activation of Chk1."Mol. Biol. Cell 22:2834-2847(2011) [PubMed] [Europe PMC] [Abstract]
  22. "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
  23. "The consensus coding sequences of human breast and colorectal cancers." Science 314:268-274(2006) [PubMed] [Europe PMC] [Abstract]