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Chromatin Assembly Factor 1, Subunit A (CHAF1A)

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CAF-1; CAF1; CAF1B; CAF1P150; P150; Chromatin assembly factor I p150 subunit

Chromatin Assembly Factor 1, Subunit A (CHAF1A)
CCC J448
HGNC 1910
MGI 1351331
PubMed 17353931
RGD 1590865
UniProt Q13111
Wiki CHAF1A
The predicted 938-amino acid protein appears to be highly charged and has an isoelectric point of 5.5. It has a PEST (proline-glutamic acid-serine-threonine) box near the N terminus that is associated with proteins of short half-lives.Mutations in CHAF1A prevented association with Hp1 in heterochromatin in cells that were not in S phase and the formation of CHAF1-Hp1 complexes in nascent chromatin during DNA replication in vitro. These data suggested that CHAF1A has a heterochromatin-specific function distinct from its nucleosome assembly function during S phase. Just before mitosis, CHAF1A and some HP1 progressively dissociate from heterochromatin concomitant with histone H3 phosphorylation. The HP1 proteins reassociate with chromatin at the end of mitosis, as histone H3 is dephosphorylated.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Chromatin Assembly Factor 1, Subunit A (CHAF1A) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Chromatin Assembly Factor 1, Subunit A (CHAF1A) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Chromatin Assembly Factor 1, Subunit A (CHAF1A) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Chromatin Assembly Factor 1, Subunit A (CHAF1A) CLIA Kit Customized Service Offer
n/a ELISA Kit for Chromatin Assembly Factor 1, Subunit A (CHAF1A) ELISA Kit Customized Service Offer

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Chromatin Assembly Factor 1, Subunit A (CHAF1A) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Chromatin Assembly Factor 1, Subunit A (CHAF1A) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Chromatin Assembly Factor 1, Subunit A (CHAF1A) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Chromatin Assembly Factor 1, Subunit A (CHAF1A) CLIA Kit Customized Service Offer
n/a ELISA Kit for Chromatin Assembly Factor 1, Subunit A (CHAF1A) ELISA Kit Customized Service Offer

Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Chromatin Assembly Factor 1, Subunit A (CHAF1A) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Chromatin Assembly Factor 1, Subunit A (CHAF1A) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Chromatin Assembly Factor 1, Subunit A (CHAF1A) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Chromatin Assembly Factor 1, Subunit A (CHAF1A) CLIA Kit Customized Service Offer
n/a ELISA Kit for Chromatin Assembly Factor 1, Subunit A (CHAF1A) ELISA Kit Customized Service Offer
  1. "Genomic sequence and expression analyses of human chromatin assembly factor 1 p150 gene."Gene 264:187-196(2001) [PubMed] [Europe PMC] [Abstract]
  2. "The DNA sequence and biology of human chromosome 19." Nature 428:529-535(2004) [PubMed] [Europe PMC] [Abstract]
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
  4. "The p150 and p60 subunits of chromatin assembly factor I: a molecular link between newly synthesized histones and DNA replication."Cell 81:1105-1114(1995) [PubMed] [Europe PMC] [Abstract]
  5. "Replication-dependent marking of DNA by PCNA facilitates CAF-1-coupled inheritance of chromatin."Cell 96:575-585(1999) [PubMed] [Europe PMC] [Abstract]
  6. "A CAF-1-PCNA-mediated chromatin assembly pathway triggered by sensing DNA damage."Mol. Cell. Biol. 20:1206-1218(2000) [PubMed] [Europe PMC] [Abstract]
  7. "CAF-1 and the inheritance of chromatin states: at the crossroads of DNA replication and repair."J. Cell Sci. 113:2647-2658(2000) [PubMed] [Europe PMC] [Abstract]
  8. "Dimerization of the largest subunit of chromatin assembly factor 1: importance in vitro and during Xenopus early development."EMBO J. 20:2015-2027(2001) [PubMed] [Europe PMC] [Abstract]
  9. "The methyl-CpG binding protein MBD1 interacts with the p150 subunit of chromatin assembly factor 1."Mol. Cell. Biol. 23:3226-3236(2003) [PubMed] [Europe PMC] [Abstract]
  10. "Methyl-CpG binding protein MBD1 couples histone H3 methylation at lysine 9 by SETDB1 to DNA replication and chromatin assembly."Mol. Cell 15:595-605(2004) [PubMed] [Europe PMC] [Abstract]
  11. "The mammalian heterochromatin protein 1 binds diverse nuclear proteins through a common motif that targets the chromoshadow domain."Biochem. Biophys. Res. Commun. 331:929-937(2005) [PubMed] [Europe PMC] [Abstract]
  12. "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract]
  13. "Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis."J. Proteome Res. 7:1346-1351(2008) [PubMed] [Europe PMC] [Abstract]
  14. "A quantitative atlas of mitotic phosphorylation."Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
  15. "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
  16. "Human POGZ modulates dissociation of HP1alpha from mitotic chromosome arms through Aurora B activation."Nat. Cell Biol. 12:719-727(2010) [PubMed] [Europe PMC] [Abstract]
  17. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
  18. "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
  19. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]