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Cell Division Cycle Associated Protein 8 (CDCA8)

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PESCRG3; BOR; MESRGP; Dasra B; Borealin; Pluripotent embryonic stem cell-related gene 3 protein

Cell Division Cycle Associated Protein 8 (CDCA8)
CCC J468
HGNC 14629
MGI 1196274
PubMed 12188893
RGD 1566306
UniProt Q53HL2
Wiki CDCA8
CDCA8 is a component of a chromosomal passenger complex required for stability of the bipolar mitotic spindle . The deduced 280-amino acid human protein, which the authors called Dasra B, shares 42% and 23% identity with Xenopus Dasra B and Dasra A, respectively. Immunofluorescence on metaphase HeLa cells revealed Dasra B in a punctate distribution on chromosomes, coincident with Aurora B (AURKB) staining. The deduced 280-amino acid protein contains 2 putative nuclear localization signals. Secondary structure predictions suggested borealin forms an N-terminal domain of 6 core helices and a C-terminal domain of at least 2 strands and 3 helices. Northern and Western blot analyses showed that borealin mRNA and protein levels were significantly increased in mitosis.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Cell Division Cycle Associated Protein 8 (CDCA8) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Cell Division Cycle Associated Protein 8 (CDCA8) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Cell Division Cycle Associated Protein 8 (CDCA8) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Cell Division Cycle Associated Protein 8 (CDCA8) CLIA Kit Customized Service Offer
n/a ELISA Kit for Cell Division Cycle Associated Protein 8 (CDCA8) ELISA Kit Customized Service Offer

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Cell Division Cycle Associated Protein 8 (CDCA8) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Cell Division Cycle Associated Protein 8 (CDCA8) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Cell Division Cycle Associated Protein 8 (CDCA8) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Cell Division Cycle Associated Protein 8 (CDCA8) CLIA Kit Customized Service Offer
n/a ELISA Kit for Cell Division Cycle Associated Protein 8 (CDCA8) ELISA Kit Customized Service Offer

Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Cell Division Cycle Associated Protein 8 (CDCA8) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Cell Division Cycle Associated Protein 8 (CDCA8) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Cell Division Cycle Associated Protein 8 (CDCA8) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Cell Division Cycle Associated Protein 8 (CDCA8) CLIA Kit Customized Service Offer
n/a ELISA Kit for Cell Division Cycle Associated Protein 8 (CDCA8) ELISA Kit Customized Service Offer
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  2. "Complete sequencing and characterization of 21,243 full-length human cDNAs." Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
  4. "The chromosomal passenger complex is required for chromatin-induced microtubule stabilization and spindle assembly."Cell 118:187-202(2004) [PubMed] [Europe PMC] [Abstract]
  5. "Borealin: a novel chromosomal passenger required for stability of the bipolar mitotic spindle."J. Cell Biol. 166:179-191(2004) [PubMed] [Europe PMC] [Abstract]
  6. "Proteome analysis of the human mitotic spindle."Mol. Cell. Proteomics 4:35-43(2005) [PubMed] [Europe PMC] [Abstract]
  7. "Survivin mediates targeting of the chromosomal passenger complex to the centromere and midbody."EMBO Rep. 7:85-92(2006) [PubMed] [Europe PMC] [Abstract]
  8. "Borealin/Dasra B is a cell cycle-regulated chromosomal passenger protein and its nuclear accumulation is linked to poor prognosis for human gastric cancer."Exp. Cell Res. 312:962-973(2006) [PubMed] [Europe PMC] [Abstract]
  9. "Molecular analysis of survivin isoforms: evidence that alternatively spliced variants do not play a role in mitosis."J. Biol. Chem. 281:1286-1295(2006) [PubMed] [Europe PMC] [Abstract]
  10. "Uncoupling the central spindle-associated function of the chromosomal passenger complex from its role at centromeres."Mol. Biol. Cell 17:1897-1909(2006) [PubMed] [Europe PMC] [Abstract]
  11. "Centromere targeting of the chromosomal passenger complex requires a ternary subcomplex of borealin, survivin, and the N-terminal domain of INCENP."Mol. Biol. Cell 17:2547-2558(2006) [PubMed] [Europe PMC] [Abstract]
  12. "Subcellular localization and nucleocytoplasmic transport of the chromosomal passenger proteins before nuclear envelope breakdown."Oncogene 25:4867-4879(2006) [PubMed] [Europe PMC] [Abstract]
  13. "Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle."Mol. Cell 31:438-448(2008) [PubMed] [Europe PMC] [Abstract]
  14. "A survivin-ran complex regulates spindle formation in tumor cells."Mol. Cell. Biol. 28:5299-5311(2008) [PubMed] [Europe PMC] [Abstract]
  15. "Mps1 phosphorylates Borealin to control Aurora B activity and chromosome alignment."Cell 132:233-246(2008) [PubMed] [Europe PMC] [Abstract]
  16. "A quantitative atlas of mitotic phosphorylation."Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
  17. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
  18. "RanBP2 and SENP3 function in a mitotic SUMO2/3 conjugation-deconjugation cycle on Borealin."Mol. Biol. Cell 20:410-418(2009) [PubMed] [Europe PMC] [Abstract]
  19. "Large-scale proteomics analysis of the human kinome."Mol. Cell. Proteomics 8:1751-1764(2009) [PubMed] [Europe PMC] [Abstract]
  20. "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
  21. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
  22. "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
  23. "Structure of a Survivin-Borealin-INCENP core complex reveals how chromosomal passengers travel together."Cell 131:271-285(2007) [PubMed] [Europe PMC] [Abstract]
  24. "Phosphorylation of a borealin dimerization domain is required for proper chromosome segregation."Biochemistry 48:6783-6793(2009) [PubMed] [Europe PMC] [Abstract]