≡

V-Akt Murine Thymoma Viral Oncogene Homolog 1 Substrate 1 (AKT1S1)

[Edit]

PRAS40; Lobe; Proline-Rich Akt Substrate,40 kDa; Proline-rich AKT1 substrate 1

V-Akt Murine Thymoma Viral Oncogene Homolog 1 Substrate 1 (AKT1S1)
CCC J651
HGNC 28426
MGI 1914855
PubMed 23415227
RGD 1312049
UniProt Q96B36
Wiki AKT1S1
AKT1S1 is a proline-rich substrate of AKT that binds 14-3-3 protein when phosphorylated. The deduced 256-amino acid human protein contains 2 N-terminal proline-rich regions and a C-terminal AKT phosphorylation site, thr246, that is conserved in rat and mouse. Northern blot analysis detected 2.6- and 1.8-kb PRAS40 transcripts in all tissues examined, with highest levels of both transcripts in liver and heart. EST database analysis suggested that these 2 transcripts are splice variants with identical coding regions.Phosphorylation induced by constitutively active AKT was not blocked by a PI3K/AKT inhibitor, unlike that induced by wildtype AKT. Mutation analysis showed that phosphorylation of PRAS40 by AKT occurred at thr246. PRAS40 and 14-3-3 protein coimmunoprecipitated in transfected HEK293 cells.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant V-Akt Murine Thymoma Viral Oncogene Homolog 1 Substrate 1 (AKT1S1) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to V-Akt Murine Thymoma Viral Oncogene Homolog 1 Substrate 1 (AKT1S1) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to V-Akt Murine Thymoma Viral Oncogene Homolog 1 Substrate 1 (AKT1S1) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for V-Akt Murine Thymoma Viral Oncogene Homolog 1 Substrate 1 (AKT1S1) CLIA Kit Customized Service Offer
n/a ELISA Kit for V-Akt Murine Thymoma Viral Oncogene Homolog 1 Substrate 1 (AKT1S1) ELISA Kit Customized Service Offer

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant V-Akt Murine Thymoma Viral Oncogene Homolog 1 Substrate 1 (AKT1S1) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to V-Akt Murine Thymoma Viral Oncogene Homolog 1 Substrate 1 (AKT1S1) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to V-Akt Murine Thymoma Viral Oncogene Homolog 1 Substrate 1 (AKT1S1) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for V-Akt Murine Thymoma Viral Oncogene Homolog 1 Substrate 1 (AKT1S1) CLIA Kit Customized Service Offer
n/a ELISA Kit for V-Akt Murine Thymoma Viral Oncogene Homolog 1 Substrate 1 (AKT1S1) ELISA Kit Customized Service Offer

Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant V-Akt Murine Thymoma Viral Oncogene Homolog 1 Substrate 1 (AKT1S1) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to V-Akt Murine Thymoma Viral Oncogene Homolog 1 Substrate 1 (AKT1S1) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to V-Akt Murine Thymoma Viral Oncogene Homolog 1 Substrate 1 (AKT1S1) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for V-Akt Murine Thymoma Viral Oncogene Homolog 1 Substrate 1 (AKT1S1) CLIA Kit Customized Service Offer
n/a ELISA Kit for V-Akt Murine Thymoma Viral Oncogene Homolog 1 Substrate 1 (AKT1S1) ELISA Kit Customized Service Offer
  1. "Complete sequencing and characterization of 21,243 full-length human cDNAs." Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
  2. "The DNA sequence and biology of human chromosome 19." Nature 428:529-535(2004) [PubMed] [Europe PMC] [Abstract]
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract], Eye and Skin.
  4. "Identification of a proline-rich Akt substrate as a 14-3-3 binding partner."J. Biol. Chem. 278:10189-10194(2003) [PubMed] [Europe PMC] [Abstract]
  5. "Expression of proline-rich Akt-substrate PRAS40 in cell survival pathway and carcinogenesis."Acta Pharmacol. Sin. 26:1253-1258(2005) [PubMed] [Europe PMC] [Abstract]
  6. "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract]
  7. "PRAS40 is an insulin-regulated inhibitor of the mTORC1 protein kinase."Mol. Cell 25:903-915(2007) [PubMed] [Europe PMC] [Abstract]
  8. "Insulin signalling to mTOR mediated by the Akt/PKB substrate PRAS40."Nat. Cell Biol. 9:316-323(2007) [PubMed] [Europe PMC] [Abstract]
  9. "A quantitative atlas of mitotic phosphorylation."Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
  10. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
  11. "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
  12. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
  13. "Initial characterization of the human central proteome."BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
  14. "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
  15. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]