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Arylformamidase (AFMID) is the second enzyme of the kynurenine pathway metabolizing tryptophan to nicotinic acid and nicotinamide adenine dinucleotide cofactors. Inhibition of AFMID by organophosphorus insecticides in developing chicken embryos is correlated with lowered NAD levels and severe teratogenesis.
The cDNA sequence previously identified for mouse liver AFMID was cloned and expressed in Escherichia coli. Residues identified as potential catalytic triad members through sequence motif and homology modeling were mutated to alanine to probe their contributions to enzyme activity. The wild-type and mutant AFMIDs were expressed as amino terminal 6 x His-tagged recombinant proteins to facilitate purification. Three chromatography steps isolated highly purified proteins for enzyme activity comparisons.
Organism species: Homo sapiens (Human)
| CATALOG NO. | PRODUCT NAME | APPLICATIONS | |
| Proteins | RPJ665Hu01 | Recombinant Arylformamidase (AFMID) | Positive Control; Immunogen; SDS-PAGE; WB. |
| Antibodies | PAJ665Hu01 | Polyclonal Antibody to Arylformamidase (AFMID) | WB; IHC; ICC; IP. |
| MAJ665Hu21 | Monoclonal Antibody to Arylformamidase (AFMID) | WB; IHC; ICC; IP. | |
| Assay Kits | SEJ665Hu | ELISA Kit for Arylformamidase (AFMID) | Enzyme-linked immunosorbent assay for Antigen Detection. |
Organism species: Mus musculus (Mouse)
| CATALOG NO. | PRODUCT NAME | APPLICATIONS | |
| Proteins | n/a | Recombinant Arylformamidase (AFMID) | Recombinant Protein Customized Service Offer |
| Antibodies | n/a | Monoclonal Antibody to Arylformamidase (AFMID) | Monoclonal Antibody Customized Service Offer |
| n/a | Polyclonal Antibody to Arylformamidase (AFMID) | Polyclonal Antibody Customized Service Offer | |
| Assay Kits | n/a | CLIA Kit for Arylformamidase (AFMID) | CLIA Kit Customized Service Offer |
| n/a | ELISA Kit for Arylformamidase (AFMID) | ELISA Kit Customized Service Offer |
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